Limits...
MicroRNA-638 inhibits cell proliferation by targeting phospholipase D1 in human gastric carcinoma.

Zhang J, Bian Z, Zhou J, Song M, Liu Z, Feng Y, Zhe L, Zhang B, Yin Y, Huang Z - Protein Cell (2015)

Bottom Line: From this research, we found that miR-638 was downregulated in GC tissues compared with corresponding noncancerous tissues (NCTs), and the DNA copy number of miR-638 was lower in GC than NCTs, which may induce the corresponding downregulation of miR-638 in GC.Subsequently, we identified that PLD1 is the target gene of miR-638 in GC, and silencing PLD1 expression phenocopied the inhibitory effect of miR-638 on GC cell proliferation.In summary, we revealed that miR-638 functions as a tumor suppressor in GC through inhibiting PLD1.

View Article: PubMed Central - PubMed

Affiliation: Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, Wuxi, 214062, China.

ABSTRACT
MicroRNAs (miRNAs) are a type of small non-coding RNAs that are often play important roles in carcinogenesis, but the carcinogenic mechanism of miRNAs is still unclear. This study will investigate the function and the mechanism of miR-638 in carcinoma (GC). The expression of miR-638 in GC and the DNA copy number of miR-638 were detected by real-time PCR. The effect of miR-638 on cell proliferation was measured by counting kit-8 assay. Different assays, including bioinformatics algorithms (TargetScan and miRanda), luciferase report assay and Western blotting, were used to identify the target gene of miR-638 in GC. The expression of miR-638 target gene in clinical CRC tissues was also validated by immunohistochemical assay. From this research, we found that miR-638 was downregulated in GC tissues compared with corresponding noncancerous tissues (NCTs), and the DNA copy number of miR-638 was lower in GC than NCTs, which may induce the corresponding downregulation of miR-638 in GC. Ectopic expression of miR-638 inhibited GC cell growth in vitro. Subsequently, we identified that PLD1 is the target gene of miR-638 in GC, and silencing PLD1 expression phenocopied the inhibitory effect of miR-638 on GC cell proliferation. Furthermore, we observed that PLD1 was overexpressed in GC tissues, and high expression of PLD1 in GC predicted poor overall survival. In summary, we revealed that miR-638 functions as a tumor suppressor in GC through inhibiting PLD1.

No MeSH data available.


Related in: MedlinePlus

MiR-638 inhibits GC cell proliferationin vitro. (A and B) Ectopic expression of miR-638 repressed the proliferation of MKN-45 and SGC-7901 cells, whereas silencing miR-638 expression enhanced the cellular growth rate of MKN-45 and SGC-7901 cells. (*P < 0.05). (C) The colony formation assay was also applied to confirm the ability of miR-638 on colony formation
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4537476&req=5

Fig2: MiR-638 inhibits GC cell proliferationin vitro. (A and B) Ectopic expression of miR-638 repressed the proliferation of MKN-45 and SGC-7901 cells, whereas silencing miR-638 expression enhanced the cellular growth rate of MKN-45 and SGC-7901 cells. (*P < 0.05). (C) The colony formation assay was also applied to confirm the ability of miR-638 on colony formation

Mentions: The down-regulation of miR-638 in GC suggests that it may involve in GC tumorigenesis. Cell proliferation assay revealed that miR-638 overexpression significantly reduced the growth rates of MKN-45 and SGC-7901 cells (P < 0.05, Fig. 2A), and colony formation assay confirmed that miR-638 inhibited the proliferation function of GC cells (P < 0.05, Fig. 2C). In contrast, silencing miR-638 expression significantly promoted the growth of MKN-45 and SGC-7901 cells (P < 0.05, Fig. 2B). In conclusion, all these data show that miR-638 has the function of growth inhibitory.Figure 2


MicroRNA-638 inhibits cell proliferation by targeting phospholipase D1 in human gastric carcinoma.

Zhang J, Bian Z, Zhou J, Song M, Liu Z, Feng Y, Zhe L, Zhang B, Yin Y, Huang Z - Protein Cell (2015)

MiR-638 inhibits GC cell proliferationin vitro. (A and B) Ectopic expression of miR-638 repressed the proliferation of MKN-45 and SGC-7901 cells, whereas silencing miR-638 expression enhanced the cellular growth rate of MKN-45 and SGC-7901 cells. (*P < 0.05). (C) The colony formation assay was also applied to confirm the ability of miR-638 on colony formation
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4537476&req=5

Fig2: MiR-638 inhibits GC cell proliferationin vitro. (A and B) Ectopic expression of miR-638 repressed the proliferation of MKN-45 and SGC-7901 cells, whereas silencing miR-638 expression enhanced the cellular growth rate of MKN-45 and SGC-7901 cells. (*P < 0.05). (C) The colony formation assay was also applied to confirm the ability of miR-638 on colony formation
Mentions: The down-regulation of miR-638 in GC suggests that it may involve in GC tumorigenesis. Cell proliferation assay revealed that miR-638 overexpression significantly reduced the growth rates of MKN-45 and SGC-7901 cells (P < 0.05, Fig. 2A), and colony formation assay confirmed that miR-638 inhibited the proliferation function of GC cells (P < 0.05, Fig. 2C). In contrast, silencing miR-638 expression significantly promoted the growth of MKN-45 and SGC-7901 cells (P < 0.05, Fig. 2B). In conclusion, all these data show that miR-638 has the function of growth inhibitory.Figure 2

Bottom Line: From this research, we found that miR-638 was downregulated in GC tissues compared with corresponding noncancerous tissues (NCTs), and the DNA copy number of miR-638 was lower in GC than NCTs, which may induce the corresponding downregulation of miR-638 in GC.Subsequently, we identified that PLD1 is the target gene of miR-638 in GC, and silencing PLD1 expression phenocopied the inhibitory effect of miR-638 on GC cell proliferation.In summary, we revealed that miR-638 functions as a tumor suppressor in GC through inhibiting PLD1.

View Article: PubMed Central - PubMed

Affiliation: Wuxi Oncology Institute, The Affiliated Hospital of Jiangnan University, Wuxi, 214062, China.

ABSTRACT
MicroRNAs (miRNAs) are a type of small non-coding RNAs that are often play important roles in carcinogenesis, but the carcinogenic mechanism of miRNAs is still unclear. This study will investigate the function and the mechanism of miR-638 in carcinoma (GC). The expression of miR-638 in GC and the DNA copy number of miR-638 were detected by real-time PCR. The effect of miR-638 on cell proliferation was measured by counting kit-8 assay. Different assays, including bioinformatics algorithms (TargetScan and miRanda), luciferase report assay and Western blotting, were used to identify the target gene of miR-638 in GC. The expression of miR-638 target gene in clinical CRC tissues was also validated by immunohistochemical assay. From this research, we found that miR-638 was downregulated in GC tissues compared with corresponding noncancerous tissues (NCTs), and the DNA copy number of miR-638 was lower in GC than NCTs, which may induce the corresponding downregulation of miR-638 in GC. Ectopic expression of miR-638 inhibited GC cell growth in vitro. Subsequently, we identified that PLD1 is the target gene of miR-638 in GC, and silencing PLD1 expression phenocopied the inhibitory effect of miR-638 on GC cell proliferation. Furthermore, we observed that PLD1 was overexpressed in GC tissues, and high expression of PLD1 in GC predicted poor overall survival. In summary, we revealed that miR-638 functions as a tumor suppressor in GC through inhibiting PLD1.

No MeSH data available.


Related in: MedlinePlus