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Persistence of Functional Sensory Maps in the Absence of Cortical Layers in the Somsatosensory Cortex of Reeler Mice.

Guy J, Wagener RJ, Möck M, Staiger JF - Cereb. Cortex (2014)

Bottom Line: We found that the loss of cortical layers in reeler mice had surprisingly little incidence on these properties.Because intrinsic imaging measures hemodynamic signals, we furthermore investigated the cortical blood vessel pattern of both genotypes, where we also did not detect major differences.In summary, the loss of the reelin protein results in a widespread disturbance of cortical development which compromises neither the establishment nor the function of an ordered, somatotopic map of the facial whiskers.

View Article: PubMed Central - PubMed

Affiliation: Institut für Neuroanatomie, Universitätsmedizin Göttingen, Georg-August-Universität, Göttingen D-37075, Germany julien.guy@med.uni-goettingen.de.

No MeSH data available.


Sensory map organization in WT and reeler somatosensory cortex. (A and C) Pseudo 3D-reconstruction of the location of the hemodynamic response evoked by single-whisker stimulation with respect to the barrel field in WTand reeler, respectively. Top: photograph of the superficial blood vessels acquired during the experiment under a 546 nm wavelength illumination (scale bar: 500 μm). Bottom: the barrel field was reconstructed postmortem from tangential sections through the barrel cortex of LIVtdTomato mice. Middle: surface plot of the averaged intrinsic signal evoked by stimulating the C1 whisker at 5 Hz for 2 s (scale bar in absolute values). The location of the peak and 90% of maximum isoline were mapped on the barrel field after aligning the blood vessels present in the reconstructions of the serial tangential sections with those present in the pial surface photograph of the living animal. (B and D) overlay of peak (x) and 90% maximum isolines locations of 9 intrinsic signals evoked by sequential stimulation of 9 whiskers in the same WT and reeler mouse, respectively. The spatial relationship between the functional intrinsic signals locations show a rough match with those of individual structural barrels. Scale bar: 250 μm.
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BHU052F3: Sensory map organization in WT and reeler somatosensory cortex. (A and C) Pseudo 3D-reconstruction of the location of the hemodynamic response evoked by single-whisker stimulation with respect to the barrel field in WTand reeler, respectively. Top: photograph of the superficial blood vessels acquired during the experiment under a 546 nm wavelength illumination (scale bar: 500 μm). Bottom: the barrel field was reconstructed postmortem from tangential sections through the barrel cortex of LIVtdTomato mice. Middle: surface plot of the averaged intrinsic signal evoked by stimulating the C1 whisker at 5 Hz for 2 s (scale bar in absolute values). The location of the peak and 90% of maximum isoline were mapped on the barrel field after aligning the blood vessels present in the reconstructions of the serial tangential sections with those present in the pial surface photograph of the living animal. (B and D) overlay of peak (x) and 90% maximum isolines locations of 9 intrinsic signals evoked by sequential stimulation of 9 whiskers in the same WT and reeler mouse, respectively. The spatial relationship between the functional intrinsic signals locations show a rough match with those of individual structural barrels. Scale bar: 250 μm.

Mentions: The data obtained from experiments on LIVtdTomato, where the localization of the functional representations of 9 individual whiskers was recorded, was processed in the following way. The 5 data frames spanning 1.2–2.2 s after stimulation onset, when the intrinsic signal is clearly visible (Fig. 2), were averaged. A Gaussian filter (σ = 86 μm) was applied to the resulting frame to improve threshold detection, and the median of the data frame was subtracted to minimize the effect of noise, especially the slow spontaneous oscillations in reflectance (Mayhew et al. 1996; Drew and Feldman 2009). In order to localize the intrinsic signals evoked by stimulation of different whiskers with minimal overlap, we extracted the contour lines encompassing the area where the signals were at 90% of their maximum (Fig. 3 and Chen-Bee et al. 2000).Figure 3.


Persistence of Functional Sensory Maps in the Absence of Cortical Layers in the Somsatosensory Cortex of Reeler Mice.

Guy J, Wagener RJ, Möck M, Staiger JF - Cereb. Cortex (2014)

Sensory map organization in WT and reeler somatosensory cortex. (A and C) Pseudo 3D-reconstruction of the location of the hemodynamic response evoked by single-whisker stimulation with respect to the barrel field in WTand reeler, respectively. Top: photograph of the superficial blood vessels acquired during the experiment under a 546 nm wavelength illumination (scale bar: 500 μm). Bottom: the barrel field was reconstructed postmortem from tangential sections through the barrel cortex of LIVtdTomato mice. Middle: surface plot of the averaged intrinsic signal evoked by stimulating the C1 whisker at 5 Hz for 2 s (scale bar in absolute values). The location of the peak and 90% of maximum isoline were mapped on the barrel field after aligning the blood vessels present in the reconstructions of the serial tangential sections with those present in the pial surface photograph of the living animal. (B and D) overlay of peak (x) and 90% maximum isolines locations of 9 intrinsic signals evoked by sequential stimulation of 9 whiskers in the same WT and reeler mouse, respectively. The spatial relationship between the functional intrinsic signals locations show a rough match with those of individual structural barrels. Scale bar: 250 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
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getmorefigures.php?uid=PMC4537421&req=5

BHU052F3: Sensory map organization in WT and reeler somatosensory cortex. (A and C) Pseudo 3D-reconstruction of the location of the hemodynamic response evoked by single-whisker stimulation with respect to the barrel field in WTand reeler, respectively. Top: photograph of the superficial blood vessels acquired during the experiment under a 546 nm wavelength illumination (scale bar: 500 μm). Bottom: the barrel field was reconstructed postmortem from tangential sections through the barrel cortex of LIVtdTomato mice. Middle: surface plot of the averaged intrinsic signal evoked by stimulating the C1 whisker at 5 Hz for 2 s (scale bar in absolute values). The location of the peak and 90% of maximum isoline were mapped on the barrel field after aligning the blood vessels present in the reconstructions of the serial tangential sections with those present in the pial surface photograph of the living animal. (B and D) overlay of peak (x) and 90% maximum isolines locations of 9 intrinsic signals evoked by sequential stimulation of 9 whiskers in the same WT and reeler mouse, respectively. The spatial relationship between the functional intrinsic signals locations show a rough match with those of individual structural barrels. Scale bar: 250 μm.
Mentions: The data obtained from experiments on LIVtdTomato, where the localization of the functional representations of 9 individual whiskers was recorded, was processed in the following way. The 5 data frames spanning 1.2–2.2 s after stimulation onset, when the intrinsic signal is clearly visible (Fig. 2), were averaged. A Gaussian filter (σ = 86 μm) was applied to the resulting frame to improve threshold detection, and the median of the data frame was subtracted to minimize the effect of noise, especially the slow spontaneous oscillations in reflectance (Mayhew et al. 1996; Drew and Feldman 2009). In order to localize the intrinsic signals evoked by stimulation of different whiskers with minimal overlap, we extracted the contour lines encompassing the area where the signals were at 90% of their maximum (Fig. 3 and Chen-Bee et al. 2000).Figure 3.

Bottom Line: We found that the loss of cortical layers in reeler mice had surprisingly little incidence on these properties.Because intrinsic imaging measures hemodynamic signals, we furthermore investigated the cortical blood vessel pattern of both genotypes, where we also did not detect major differences.In summary, the loss of the reelin protein results in a widespread disturbance of cortical development which compromises neither the establishment nor the function of an ordered, somatotopic map of the facial whiskers.

View Article: PubMed Central - PubMed

Affiliation: Institut für Neuroanatomie, Universitätsmedizin Göttingen, Georg-August-Universität, Göttingen D-37075, Germany julien.guy@med.uni-goettingen.de.

No MeSH data available.