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Persistence of Functional Sensory Maps in the Absence of Cortical Layers in the Somsatosensory Cortex of Reeler Mice.

Guy J, Wagener RJ, Möck M, Staiger JF - Cereb. Cortex (2014)

Bottom Line: We found that the loss of cortical layers in reeler mice had surprisingly little incidence on these properties.Because intrinsic imaging measures hemodynamic signals, we furthermore investigated the cortical blood vessel pattern of both genotypes, where we also did not detect major differences.In summary, the loss of the reelin protein results in a widespread disturbance of cortical development which compromises neither the establishment nor the function of an ordered, somatotopic map of the facial whiskers.

View Article: PubMed Central - PubMed

Affiliation: Institut für Neuroanatomie, Universitätsmedizin Göttingen, Georg-August-Universität, Göttingen D-37075, Germany julien.guy@med.uni-goettingen.de.

No MeSH data available.


The hemodynamic response to single-whisker stimulation in WT and reeler mice. (A) Time series of camera frames illustrating the response to stimulation of whisker C2 at 5 Hz for 2 s in 2 representative examples of WT and reeler animals. Stimulation starts at time 0, each frame represents 200 ms. The camera frame immediately before stimulation onset was used as reference for normalization; the scale bar represents fractional change in reflectance with respect to that frame. Stimulation evoked a localized hemodynamic response corresponding to the sensory representation of whisker C2 in WT but also in reeler mice. Neither the mean latency nor mean duration of the evoked intrinsic signal differed significantly between genotypes (one-way ANOVA, P = 0.7 and P = 0.46, respectively). (B and C) Left, photograph of the surface vasculature in 2 representative examples corresponding to the data in A. SCV, superior cerebral vein, MCA, middle cerebral artery, M, medial, L, lateral, A, anterior, P, posterior. Scale bar: 500 μm. Right, sensory maps obtained by binning 15 frames spanning the period from 1 to 4 s after stimulation, applying Gaussian blur with σ = 86 μm and subtracting the median of the filtered image.
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BHU052F2: The hemodynamic response to single-whisker stimulation in WT and reeler mice. (A) Time series of camera frames illustrating the response to stimulation of whisker C2 at 5 Hz for 2 s in 2 representative examples of WT and reeler animals. Stimulation starts at time 0, each frame represents 200 ms. The camera frame immediately before stimulation onset was used as reference for normalization; the scale bar represents fractional change in reflectance with respect to that frame. Stimulation evoked a localized hemodynamic response corresponding to the sensory representation of whisker C2 in WT but also in reeler mice. Neither the mean latency nor mean duration of the evoked intrinsic signal differed significantly between genotypes (one-way ANOVA, P = 0.7 and P = 0.46, respectively). (B and C) Left, photograph of the surface vasculature in 2 representative examples corresponding to the data in A. SCV, superior cerebral vein, MCA, middle cerebral artery, M, medial, L, lateral, A, anterior, P, posterior. Scale bar: 500 μm. Right, sensory maps obtained by binning 15 frames spanning the period from 1 to 4 s after stimulation, applying Gaussian blur with σ = 86 μm and subtracting the median of the filtered image.

Mentions: The data obtained from experiments on LIVtdTomato, where the localization of the functional representations of 9 individual whiskers was recorded, was processed in the following way. The 5 data frames spanning 1.2–2.2 s after stimulation onset, when the intrinsic signal is clearly visible (Fig. 2), were averaged. A Gaussian filter (σ = 86 μm) was applied to the resulting frame to improve threshold detection, and the median of the data frame was subtracted to minimize the effect of noise, especially the slow spontaneous oscillations in reflectance (Mayhew et al. 1996; Drew and Feldman 2009). In order to localize the intrinsic signals evoked by stimulation of different whiskers with minimal overlap, we extracted the contour lines encompassing the area where the signals were at 90% of their maximum (Fig. 3 and Chen-Bee et al. 2000).Figure 3.


Persistence of Functional Sensory Maps in the Absence of Cortical Layers in the Somsatosensory Cortex of Reeler Mice.

Guy J, Wagener RJ, Möck M, Staiger JF - Cereb. Cortex (2014)

The hemodynamic response to single-whisker stimulation in WT and reeler mice. (A) Time series of camera frames illustrating the response to stimulation of whisker C2 at 5 Hz for 2 s in 2 representative examples of WT and reeler animals. Stimulation starts at time 0, each frame represents 200 ms. The camera frame immediately before stimulation onset was used as reference for normalization; the scale bar represents fractional change in reflectance with respect to that frame. Stimulation evoked a localized hemodynamic response corresponding to the sensory representation of whisker C2 in WT but also in reeler mice. Neither the mean latency nor mean duration of the evoked intrinsic signal differed significantly between genotypes (one-way ANOVA, P = 0.7 and P = 0.46, respectively). (B and C) Left, photograph of the surface vasculature in 2 representative examples corresponding to the data in A. SCV, superior cerebral vein, MCA, middle cerebral artery, M, medial, L, lateral, A, anterior, P, posterior. Scale bar: 500 μm. Right, sensory maps obtained by binning 15 frames spanning the period from 1 to 4 s after stimulation, applying Gaussian blur with σ = 86 μm and subtracting the median of the filtered image.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4537421&req=5

BHU052F2: The hemodynamic response to single-whisker stimulation in WT and reeler mice. (A) Time series of camera frames illustrating the response to stimulation of whisker C2 at 5 Hz for 2 s in 2 representative examples of WT and reeler animals. Stimulation starts at time 0, each frame represents 200 ms. The camera frame immediately before stimulation onset was used as reference for normalization; the scale bar represents fractional change in reflectance with respect to that frame. Stimulation evoked a localized hemodynamic response corresponding to the sensory representation of whisker C2 in WT but also in reeler mice. Neither the mean latency nor mean duration of the evoked intrinsic signal differed significantly between genotypes (one-way ANOVA, P = 0.7 and P = 0.46, respectively). (B and C) Left, photograph of the surface vasculature in 2 representative examples corresponding to the data in A. SCV, superior cerebral vein, MCA, middle cerebral artery, M, medial, L, lateral, A, anterior, P, posterior. Scale bar: 500 μm. Right, sensory maps obtained by binning 15 frames spanning the period from 1 to 4 s after stimulation, applying Gaussian blur with σ = 86 μm and subtracting the median of the filtered image.
Mentions: The data obtained from experiments on LIVtdTomato, where the localization of the functional representations of 9 individual whiskers was recorded, was processed in the following way. The 5 data frames spanning 1.2–2.2 s after stimulation onset, when the intrinsic signal is clearly visible (Fig. 2), were averaged. A Gaussian filter (σ = 86 μm) was applied to the resulting frame to improve threshold detection, and the median of the data frame was subtracted to minimize the effect of noise, especially the slow spontaneous oscillations in reflectance (Mayhew et al. 1996; Drew and Feldman 2009). In order to localize the intrinsic signals evoked by stimulation of different whiskers with minimal overlap, we extracted the contour lines encompassing the area where the signals were at 90% of their maximum (Fig. 3 and Chen-Bee et al. 2000).Figure 3.

Bottom Line: We found that the loss of cortical layers in reeler mice had surprisingly little incidence on these properties.Because intrinsic imaging measures hemodynamic signals, we furthermore investigated the cortical blood vessel pattern of both genotypes, where we also did not detect major differences.In summary, the loss of the reelin protein results in a widespread disturbance of cortical development which compromises neither the establishment nor the function of an ordered, somatotopic map of the facial whiskers.

View Article: PubMed Central - PubMed

Affiliation: Institut für Neuroanatomie, Universitätsmedizin Göttingen, Georg-August-Universität, Göttingen D-37075, Germany julien.guy@med.uni-goettingen.de.

No MeSH data available.