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Determine the structure of phosphorylated modification of icariin and its antiviral activity against duck hepatitis virus A.

Xiong W, Ma X, Wu Y, Chen Y, Zeng L, Liu J, Sun W, Wang D, Hu Y - BMC Vet. Res. (2015)

Bottom Line: Anti-DHAV activities of 1 and 2 were compared in duck embryonic hepatocytes (DEHs) cultured in vitro and by artificial infection method in vivo.The compound 2's chemical structure was defined as 8-prenylkaempferol-4'-methylether-3-rhamnosyl-7-(6'''-phosphate)-glycoside. 1 and 2 exhibited anti-virus activity on DHAV.Our results suggest that 1 and 2 might become an anti-virus plant material candidate.

View Article: PubMed Central - PubMed

Affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. pansquito@hotmail.com.

ABSTRACT

Background: Our previous research showed that icariin (1) and its phosphorylated structural modification (2) improved the survival and attenuated oxidative stress and liver dysfunction induced by duck virus hepatitis. In this paper, we were one step closer to determine the structure of phosphorylation icariin (2) by the FT-IR, HRESIMS and (13)C NMR. Anti-DHAV activities of 1 and 2 were compared in duck embryonic hepatocytes (DEHs) cultured in vitro and by artificial infection method in vivo. Additionally, the antiviral mechanisms of replication/release in vitro and the DHAV gene expression in vivo of 1 and 2 were analyzed.

Results: Compound 2's molecular formula was C33H42O18P. The results indicated that 1 and 2 effectively resisted DHAV invading DEHs, that they decreased the mortality of ducklings challenged with DHAV, and that 2 performed more effectively. 1 and 2 performed evenly on DHAV release; however, 2 restrained virus replication far more effectively. Since the anti-DHAV mechanisms of 1 and 2 in vitro probably involve suppression of replication and release, 2's better performance in anti-DHAV may result from its far more effectively inhibiting virus replication.

Conclusions: The compound 2's chemical structure was defined as 8-prenylkaempferol-4'-methylether-3-rhamnosyl-7-(6'''-phosphate)-glycoside. 1 and 2 exhibited anti-virus activity on DHAV. Our results suggest that 1 and 2 might become an anti-virus plant material candidate.

No MeSH data available.


Related in: MedlinePlus

Quantitative analysis of the DHAV gene expression in blood at the 4th, the 8th and the 54th h after injected virus. DHAV gene expression of the VC group at 4 h after injected virus was set to 1 and that of the BC group was set to 0. The different letters on a column differ significantly (p < 0.05)
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Fig3: Quantitative analysis of the DHAV gene expression in blood at the 4th, the 8th and the 54th h after injected virus. DHAV gene expression of the VC group at 4 h after injected virus was set to 1 and that of the BC group was set to 0. The different letters on a column differ significantly (p < 0.05)

Mentions: A quantitative analysis of the DHAV gene expression in blood at the 4th, the 8th and the 54th h after injected with virus was illustrated in Fig. 3. No DHAV gene expression was found in the BC group. DHAV gene expression of the VC group in the 4th h after injected virus was set to 1 and that of the BC group was set to 0. The VC group dynamics indicated that the DHAV content first increased and then decreased in vivo of ducklings, with the pattern of the 1 and 2 groups similar to that of the VC group. The relative expressions of DHAV gene in blood of the 1 and 2 groups were lower than that of the VC group during the same period. Four hours after DHAV injection, the virus gene expressions in both the 1 and 2 groups were lower than that of the VC group. At the 8th hour after DHAV injection the virus contents of the 1 and 2 groups were lower than that of the VC group with significant difference. At the 54th h after DHAV injection the virus gene content of the 2 group was remarkably lower than that of the VC group.Fig. 3


Determine the structure of phosphorylated modification of icariin and its antiviral activity against duck hepatitis virus A.

Xiong W, Ma X, Wu Y, Chen Y, Zeng L, Liu J, Sun W, Wang D, Hu Y - BMC Vet. Res. (2015)

Quantitative analysis of the DHAV gene expression in blood at the 4th, the 8th and the 54th h after injected virus. DHAV gene expression of the VC group at 4 h after injected virus was set to 1 and that of the BC group was set to 0. The different letters on a column differ significantly (p < 0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536795&req=5

Fig3: Quantitative analysis of the DHAV gene expression in blood at the 4th, the 8th and the 54th h after injected virus. DHAV gene expression of the VC group at 4 h after injected virus was set to 1 and that of the BC group was set to 0. The different letters on a column differ significantly (p < 0.05)
Mentions: A quantitative analysis of the DHAV gene expression in blood at the 4th, the 8th and the 54th h after injected with virus was illustrated in Fig. 3. No DHAV gene expression was found in the BC group. DHAV gene expression of the VC group in the 4th h after injected virus was set to 1 and that of the BC group was set to 0. The VC group dynamics indicated that the DHAV content first increased and then decreased in vivo of ducklings, with the pattern of the 1 and 2 groups similar to that of the VC group. The relative expressions of DHAV gene in blood of the 1 and 2 groups were lower than that of the VC group during the same period. Four hours after DHAV injection, the virus gene expressions in both the 1 and 2 groups were lower than that of the VC group. At the 8th hour after DHAV injection the virus contents of the 1 and 2 groups were lower than that of the VC group with significant difference. At the 54th h after DHAV injection the virus gene content of the 2 group was remarkably lower than that of the VC group.Fig. 3

Bottom Line: Anti-DHAV activities of 1 and 2 were compared in duck embryonic hepatocytes (DEHs) cultured in vitro and by artificial infection method in vivo.The compound 2's chemical structure was defined as 8-prenylkaempferol-4'-methylether-3-rhamnosyl-7-(6'''-phosphate)-glycoside. 1 and 2 exhibited anti-virus activity on DHAV.Our results suggest that 1 and 2 might become an anti-virus plant material candidate.

View Article: PubMed Central - PubMed

Affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. pansquito@hotmail.com.

ABSTRACT

Background: Our previous research showed that icariin (1) and its phosphorylated structural modification (2) improved the survival and attenuated oxidative stress and liver dysfunction induced by duck virus hepatitis. In this paper, we were one step closer to determine the structure of phosphorylation icariin (2) by the FT-IR, HRESIMS and (13)C NMR. Anti-DHAV activities of 1 and 2 were compared in duck embryonic hepatocytes (DEHs) cultured in vitro and by artificial infection method in vivo. Additionally, the antiviral mechanisms of replication/release in vitro and the DHAV gene expression in vivo of 1 and 2 were analyzed.

Results: Compound 2's molecular formula was C33H42O18P. The results indicated that 1 and 2 effectively resisted DHAV invading DEHs, that they decreased the mortality of ducklings challenged with DHAV, and that 2 performed more effectively. 1 and 2 performed evenly on DHAV release; however, 2 restrained virus replication far more effectively. Since the anti-DHAV mechanisms of 1 and 2 in vitro probably involve suppression of replication and release, 2's better performance in anti-DHAV may result from its far more effectively inhibiting virus replication.

Conclusions: The compound 2's chemical structure was defined as 8-prenylkaempferol-4'-methylether-3-rhamnosyl-7-(6'''-phosphate)-glycoside. 1 and 2 exhibited anti-virus activity on DHAV. Our results suggest that 1 and 2 might become an anti-virus plant material candidate.

No MeSH data available.


Related in: MedlinePlus