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PATRONUS1 is expressed in meiotic prophase I to regulate centromeric cohesion in Arabidopsis and shows synthetic lethality with OSD1.

Singh DK, Spillane C, Siddiqi I - BMC Plant Biol. (2015)

Bottom Line: Retention of sister centromere cohesion during meiosis I and its dissolution at meiosis II is necessary for balanced chromosome segregation and reduction of chromosome number.We show here that PANS1 protein is found mainly in prophase I of meiosis, with its level declining late in prophase I during diplotene.PANS1 also shows expression in dividing tissues.

View Article: PubMed Central - PubMed

Affiliation: Centre for Cellular and Molecular Biology (CSIR), Uppal Road, Hyderabad, 500007, India. dipesh@ccmb.res.in.

ABSTRACT

Background: Retention of sister centromere cohesion during meiosis I and its dissolution at meiosis II is necessary for balanced chromosome segregation and reduction of chromosome number. PATRONUS1 (PANS1) has recently been proposed to regulate centromere cohesion in Arabidopsis after meiosis I, during interkinesis. pans1 mutants lose centromere cohesion prematurely during interkinesis and segregate randomly at meiosis II. PANS1 protein interacts with components of the Anaphase Promoting Complex/Cyclosome (APC/C).

Results: We show here that PANS1 protein is found mainly in prophase I of meiosis, with its level declining late in prophase I during diplotene. PANS1 also shows expression in dividing tissues. We demonstrate that, in addition to the previously reported premature loss of centromere cohesion during interkinesis, pans1 mutants show partially penetrant defects in centromere cohesion during meiosis I. We also determine that pans1 shows synthetic lethality at the level of the sporophyte, with Omission of Second Division 1 (osd1), which encodes a known inhibitor of the APC/C that is required for cell cycle progression during mitosis, as well as meiosis I and II.

Conclusions: Our results show that PANS1 is expressed mainly in meiosis I where it has an important function and together with previous studies indicate that PANS1 and OSD1 are part of a network linking centromere cohesion and cell cycle progression through control of APC/C activity.

No MeSH data available.


Related in: MedlinePlus

PANS1-FLAG is localized to the nucleus during prophase I of Meiosis. Immunostaining of anther squashes of PANS1-FLAG transgenic line, showing chromosomes stained with DAPI (cyan), FLAG (green) and merged images for cyan and green channels (right column).a-c Early prophase I, d-f Mid prophase I, g-i Late prophase I
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Fig4: PANS1-FLAG is localized to the nucleus during prophase I of Meiosis. Immunostaining of anther squashes of PANS1-FLAG transgenic line, showing chromosomes stained with DAPI (cyan), FLAG (green) and merged images for cyan and green channels (right column).a-c Early prophase I, d-f Mid prophase I, g-i Late prophase I

Mentions: To obtain more detailed information on subcellular localization of PANS1 in meiocytes, we generated a FLAG-tagged PANS1 line in a pans1 mutant background in which the pans1 mutant was complemented by the FLAG-tagged allele. We then examined protein localization in meiosis using an anti-FLAG antibody. A strong PANS1-FLAG signal was observed up to mid-prophase and declined during late prophase (Fig. 4; Additional file 1: Figure S3). The pattern of localization extended broadly across the nucleus but appeared to be excluded from the nucleolus. The timing of maximal PANS1 expression during meiosis therefore appears to precede the onset of the mutant phenotype (comprising defects in centromere organization starting late in meiosis I and extending to interkinesis during the second meiotic division).Fig. 4


PATRONUS1 is expressed in meiotic prophase I to regulate centromeric cohesion in Arabidopsis and shows synthetic lethality with OSD1.

Singh DK, Spillane C, Siddiqi I - BMC Plant Biol. (2015)

PANS1-FLAG is localized to the nucleus during prophase I of Meiosis. Immunostaining of anther squashes of PANS1-FLAG transgenic line, showing chromosomes stained with DAPI (cyan), FLAG (green) and merged images for cyan and green channels (right column).a-c Early prophase I, d-f Mid prophase I, g-i Late prophase I
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536785&req=5

Fig4: PANS1-FLAG is localized to the nucleus during prophase I of Meiosis. Immunostaining of anther squashes of PANS1-FLAG transgenic line, showing chromosomes stained with DAPI (cyan), FLAG (green) and merged images for cyan and green channels (right column).a-c Early prophase I, d-f Mid prophase I, g-i Late prophase I
Mentions: To obtain more detailed information on subcellular localization of PANS1 in meiocytes, we generated a FLAG-tagged PANS1 line in a pans1 mutant background in which the pans1 mutant was complemented by the FLAG-tagged allele. We then examined protein localization in meiosis using an anti-FLAG antibody. A strong PANS1-FLAG signal was observed up to mid-prophase and declined during late prophase (Fig. 4; Additional file 1: Figure S3). The pattern of localization extended broadly across the nucleus but appeared to be excluded from the nucleolus. The timing of maximal PANS1 expression during meiosis therefore appears to precede the onset of the mutant phenotype (comprising defects in centromere organization starting late in meiosis I and extending to interkinesis during the second meiotic division).Fig. 4

Bottom Line: Retention of sister centromere cohesion during meiosis I and its dissolution at meiosis II is necessary for balanced chromosome segregation and reduction of chromosome number.We show here that PANS1 protein is found mainly in prophase I of meiosis, with its level declining late in prophase I during diplotene.PANS1 also shows expression in dividing tissues.

View Article: PubMed Central - PubMed

Affiliation: Centre for Cellular and Molecular Biology (CSIR), Uppal Road, Hyderabad, 500007, India. dipesh@ccmb.res.in.

ABSTRACT

Background: Retention of sister centromere cohesion during meiosis I and its dissolution at meiosis II is necessary for balanced chromosome segregation and reduction of chromosome number. PATRONUS1 (PANS1) has recently been proposed to regulate centromere cohesion in Arabidopsis after meiosis I, during interkinesis. pans1 mutants lose centromere cohesion prematurely during interkinesis and segregate randomly at meiosis II. PANS1 protein interacts with components of the Anaphase Promoting Complex/Cyclosome (APC/C).

Results: We show here that PANS1 protein is found mainly in prophase I of meiosis, with its level declining late in prophase I during diplotene. PANS1 also shows expression in dividing tissues. We demonstrate that, in addition to the previously reported premature loss of centromere cohesion during interkinesis, pans1 mutants show partially penetrant defects in centromere cohesion during meiosis I. We also determine that pans1 shows synthetic lethality at the level of the sporophyte, with Omission of Second Division 1 (osd1), which encodes a known inhibitor of the APC/C that is required for cell cycle progression during mitosis, as well as meiosis I and II.

Conclusions: Our results show that PANS1 is expressed mainly in meiosis I where it has an important function and together with previous studies indicate that PANS1 and OSD1 are part of a network linking centromere cohesion and cell cycle progression through control of APC/C activity.

No MeSH data available.


Related in: MedlinePlus