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PATRONUS1 is expressed in meiotic prophase I to regulate centromeric cohesion in Arabidopsis and shows synthetic lethality with OSD1.

Singh DK, Spillane C, Siddiqi I - BMC Plant Biol. (2015)

Bottom Line: Retention of sister centromere cohesion during meiosis I and its dissolution at meiosis II is necessary for balanced chromosome segregation and reduction of chromosome number.We show here that PANS1 protein is found mainly in prophase I of meiosis, with its level declining late in prophase I during diplotene.PANS1 also shows expression in dividing tissues.

View Article: PubMed Central - PubMed

Affiliation: Centre for Cellular and Molecular Biology (CSIR), Uppal Road, Hyderabad, 500007, India. dipesh@ccmb.res.in.

ABSTRACT

Background: Retention of sister centromere cohesion during meiosis I and its dissolution at meiosis II is necessary for balanced chromosome segregation and reduction of chromosome number. PATRONUS1 (PANS1) has recently been proposed to regulate centromere cohesion in Arabidopsis after meiosis I, during interkinesis. pans1 mutants lose centromere cohesion prematurely during interkinesis and segregate randomly at meiosis II. PANS1 protein interacts with components of the Anaphase Promoting Complex/Cyclosome (APC/C).

Results: We show here that PANS1 protein is found mainly in prophase I of meiosis, with its level declining late in prophase I during diplotene. PANS1 also shows expression in dividing tissues. We demonstrate that, in addition to the previously reported premature loss of centromere cohesion during interkinesis, pans1 mutants show partially penetrant defects in centromere cohesion during meiosis I. We also determine that pans1 shows synthetic lethality at the level of the sporophyte, with Omission of Second Division 1 (osd1), which encodes a known inhibitor of the APC/C that is required for cell cycle progression during mitosis, as well as meiosis I and II.

Conclusions: Our results show that PANS1 is expressed mainly in meiosis I where it has an important function and together with previous studies indicate that PANS1 and OSD1 are part of a network linking centromere cohesion and cell cycle progression through control of APC/C activity.

No MeSH data available.


Related in: MedlinePlus

PANS1 is expressed in actively dividing tissue. Ppans1.PANS1-GFP-GUS fusion plants shows GUS expression in dividing tissues. a Shoot meristematic region and cauline leaves. b Young cauline leaves showing GUS expression in basal portion. c Root d GUS expression in axillary buds but not in adult cauline leaves. e High levels of expression in developing anthers and pistil. f Inflorescence g Meiotic stage anther. h Quantitative reverse transcription PCR (q-RT-PCR) of PANS1. Columns indicate the mean of levels of expression, error bars indicate standard deviation. Scale bar represents 100 μm in (e) and 20 μm in (g)
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Fig3: PANS1 is expressed in actively dividing tissue. Ppans1.PANS1-GFP-GUS fusion plants shows GUS expression in dividing tissues. a Shoot meristematic region and cauline leaves. b Young cauline leaves showing GUS expression in basal portion. c Root d GUS expression in axillary buds but not in adult cauline leaves. e High levels of expression in developing anthers and pistil. f Inflorescence g Meiotic stage anther. h Quantitative reverse transcription PCR (q-RT-PCR) of PANS1. Columns indicate the mean of levels of expression, error bars indicate standard deviation. Scale bar represents 100 μm in (e) and 20 μm in (g)

Mentions: A genomic fragment comprising the PANS1 promoter and coding region was fused to a composite Green Fluorescent Protein-Beta Glucuronidase (GFP-GUS) reporter to generate a fusion protein and the transgene cassette transformed into plants. Analysis of GUS reporter gene expression indicated that PANS1 shows increased expression in growing parts of the plant, being expressed in inflorescence, young buds, and roots (Fig. 3). Expression declined in older buds and was observed in the basal but not distal portion of young leaves coincident with the pattern of cessation of cell division in leaves which proceeds from tip to base. Quantitative analysis of gene expression indicated that PANS1 is strongly expressed in the inflorescence and at a lower level in leaves and in roots.Fig. 3


PATRONUS1 is expressed in meiotic prophase I to regulate centromeric cohesion in Arabidopsis and shows synthetic lethality with OSD1.

Singh DK, Spillane C, Siddiqi I - BMC Plant Biol. (2015)

PANS1 is expressed in actively dividing tissue. Ppans1.PANS1-GFP-GUS fusion plants shows GUS expression in dividing tissues. a Shoot meristematic region and cauline leaves. b Young cauline leaves showing GUS expression in basal portion. c Root d GUS expression in axillary buds but not in adult cauline leaves. e High levels of expression in developing anthers and pistil. f Inflorescence g Meiotic stage anther. h Quantitative reverse transcription PCR (q-RT-PCR) of PANS1. Columns indicate the mean of levels of expression, error bars indicate standard deviation. Scale bar represents 100 μm in (e) and 20 μm in (g)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536785&req=5

Fig3: PANS1 is expressed in actively dividing tissue. Ppans1.PANS1-GFP-GUS fusion plants shows GUS expression in dividing tissues. a Shoot meristematic region and cauline leaves. b Young cauline leaves showing GUS expression in basal portion. c Root d GUS expression in axillary buds but not in adult cauline leaves. e High levels of expression in developing anthers and pistil. f Inflorescence g Meiotic stage anther. h Quantitative reverse transcription PCR (q-RT-PCR) of PANS1. Columns indicate the mean of levels of expression, error bars indicate standard deviation. Scale bar represents 100 μm in (e) and 20 μm in (g)
Mentions: A genomic fragment comprising the PANS1 promoter and coding region was fused to a composite Green Fluorescent Protein-Beta Glucuronidase (GFP-GUS) reporter to generate a fusion protein and the transgene cassette transformed into plants. Analysis of GUS reporter gene expression indicated that PANS1 shows increased expression in growing parts of the plant, being expressed in inflorescence, young buds, and roots (Fig. 3). Expression declined in older buds and was observed in the basal but not distal portion of young leaves coincident with the pattern of cessation of cell division in leaves which proceeds from tip to base. Quantitative analysis of gene expression indicated that PANS1 is strongly expressed in the inflorescence and at a lower level in leaves and in roots.Fig. 3

Bottom Line: Retention of sister centromere cohesion during meiosis I and its dissolution at meiosis II is necessary for balanced chromosome segregation and reduction of chromosome number.We show here that PANS1 protein is found mainly in prophase I of meiosis, with its level declining late in prophase I during diplotene.PANS1 also shows expression in dividing tissues.

View Article: PubMed Central - PubMed

Affiliation: Centre for Cellular and Molecular Biology (CSIR), Uppal Road, Hyderabad, 500007, India. dipesh@ccmb.res.in.

ABSTRACT

Background: Retention of sister centromere cohesion during meiosis I and its dissolution at meiosis II is necessary for balanced chromosome segregation and reduction of chromosome number. PATRONUS1 (PANS1) has recently been proposed to regulate centromere cohesion in Arabidopsis after meiosis I, during interkinesis. pans1 mutants lose centromere cohesion prematurely during interkinesis and segregate randomly at meiosis II. PANS1 protein interacts with components of the Anaphase Promoting Complex/Cyclosome (APC/C).

Results: We show here that PANS1 protein is found mainly in prophase I of meiosis, with its level declining late in prophase I during diplotene. PANS1 also shows expression in dividing tissues. We demonstrate that, in addition to the previously reported premature loss of centromere cohesion during interkinesis, pans1 mutants show partially penetrant defects in centromere cohesion during meiosis I. We also determine that pans1 shows synthetic lethality at the level of the sporophyte, with Omission of Second Division 1 (osd1), which encodes a known inhibitor of the APC/C that is required for cell cycle progression during mitosis, as well as meiosis I and II.

Conclusions: Our results show that PANS1 is expressed mainly in meiosis I where it has an important function and together with previous studies indicate that PANS1 and OSD1 are part of a network linking centromere cohesion and cell cycle progression through control of APC/C activity.

No MeSH data available.


Related in: MedlinePlus