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Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient.

Al-Shammari AM, Alshami MA, Umran MA, Almukhtar AA, Yaseen NY, Raad K, Hussien AA - Breast Cancer (Dove Med Press) (2015)

Bottom Line: The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature.Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression.AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin.

View Article: PubMed Central - PubMed

Affiliation: Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, Baghdad, Iraq.

ABSTRACT
A new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient. It is considered unique because it is the first for an Iraqi population, and is expected to be a useful tool in breast cancer research. The AMJ13 cell line was established from the primary tumor of a 70-year-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. The cells were morphologically characterized by light and scanning electron microscopy, and found to be elongated multipolar epithelial-like cells with a population doubling time of 22 hours. The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature. Cytogenetic study of these cells showed chromosomal aberrations with many structural and numerical abnormalities, producing chromosomes of unknown origin called marker chromosomes. Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression. AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin. This cell line should be useful when testing new therapies for breast cancer in the Middle East.

No MeSH data available.


Related in: MedlinePlus

Histogram showing abnormal chromosome numbers in AMJ13 cells.
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f7-bctt-7-223: Histogram showing abnormal chromosome numbers in AMJ13 cells.

Mentions: The chromosomal aberrations of the new established breast cancer cell line were both numerical and structural. Chromosomal numbers differed between the cells (Figure 5) that ranged from 31 to 45 were 44%, this represented the majority of cells in the population on the slide, second group that revealed 31% percentage of cells with chromosome number (<30) and 25% been cells with chromosome number more than 47 (Figure 6 and 7).


Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient.

Al-Shammari AM, Alshami MA, Umran MA, Almukhtar AA, Yaseen NY, Raad K, Hussien AA - Breast Cancer (Dove Med Press) (2015)

Histogram showing abnormal chromosome numbers in AMJ13 cells.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4536763&req=5

f7-bctt-7-223: Histogram showing abnormal chromosome numbers in AMJ13 cells.
Mentions: The chromosomal aberrations of the new established breast cancer cell line were both numerical and structural. Chromosomal numbers differed between the cells (Figure 5) that ranged from 31 to 45 were 44%, this represented the majority of cells in the population on the slide, second group that revealed 31% percentage of cells with chromosome number (<30) and 25% been cells with chromosome number more than 47 (Figure 6 and 7).

Bottom Line: The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature.Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression.AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin.

View Article: PubMed Central - PubMed

Affiliation: Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, Baghdad, Iraq.

ABSTRACT
A new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient. It is considered unique because it is the first for an Iraqi population, and is expected to be a useful tool in breast cancer research. The AMJ13 cell line was established from the primary tumor of a 70-year-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. The cells were morphologically characterized by light and scanning electron microscopy, and found to be elongated multipolar epithelial-like cells with a population doubling time of 22 hours. The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature. Cytogenetic study of these cells showed chromosomal aberrations with many structural and numerical abnormalities, producing chromosomes of unknown origin called marker chromosomes. Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression. AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin. This cell line should be useful when testing new therapies for breast cancer in the Middle East.

No MeSH data available.


Related in: MedlinePlus