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Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient.

Al-Shammari AM, Alshami MA, Umran MA, Almukhtar AA, Yaseen NY, Raad K, Hussien AA - Breast Cancer (Dove Med Press) (2015)

Bottom Line: The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature.Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression.AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin.

View Article: PubMed Central - PubMed

Affiliation: Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, Baghdad, Iraq.

ABSTRACT
A new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient. It is considered unique because it is the first for an Iraqi population, and is expected to be a useful tool in breast cancer research. The AMJ13 cell line was established from the primary tumor of a 70-year-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. The cells were morphologically characterized by light and scanning electron microscopy, and found to be elongated multipolar epithelial-like cells with a population doubling time of 22 hours. The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature. Cytogenetic study of these cells showed chromosomal aberrations with many structural and numerical abnormalities, producing chromosomes of unknown origin called marker chromosomes. Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression. AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin. This cell line should be useful when testing new therapies for breast cancer in the Middle East.

No MeSH data available.


Related in: MedlinePlus

Growth curve for AMJ13 cell line, with a population doubling time of 22 hours.
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f3-bctt-7-223: Growth curve for AMJ13 cell line, with a population doubling time of 22 hours.

Mentions: The growth kinetics of the AMJ13 breast cancer cell line were studied at passage 10, and the cell growth curve is shown in Figure 3. The population doubling time was calculated to be 22 hours.


Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient.

Al-Shammari AM, Alshami MA, Umran MA, Almukhtar AA, Yaseen NY, Raad K, Hussien AA - Breast Cancer (Dove Med Press) (2015)

Growth curve for AMJ13 cell line, with a population doubling time of 22 hours.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4536763&req=5

f3-bctt-7-223: Growth curve for AMJ13 cell line, with a population doubling time of 22 hours.
Mentions: The growth kinetics of the AMJ13 breast cancer cell line were studied at passage 10, and the cell growth curve is shown in Figure 3. The population doubling time was calculated to be 22 hours.

Bottom Line: The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature.Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression.AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin.

View Article: PubMed Central - PubMed

Affiliation: Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, Baghdad, Iraq.

ABSTRACT
A new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient. It is considered unique because it is the first for an Iraqi population, and is expected to be a useful tool in breast cancer research. The AMJ13 cell line was established from the primary tumor of a 70-year-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. The cells were morphologically characterized by light and scanning electron microscopy, and found to be elongated multipolar epithelial-like cells with a population doubling time of 22 hours. The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature. Cytogenetic study of these cells showed chromosomal aberrations with many structural and numerical abnormalities, producing chromosomes of unknown origin called marker chromosomes. Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression. AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin. This cell line should be useful when testing new therapies for breast cancer in the Middle East.

No MeSH data available.


Related in: MedlinePlus