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Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient.

Al-Shammari AM, Alshami MA, Umran MA, Almukhtar AA, Yaseen NY, Raad K, Hussien AA - Breast Cancer (Dove Med Press) (2015)

Bottom Line: The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature.Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression.AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin.

View Article: PubMed Central - PubMed

Affiliation: Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, Baghdad, Iraq.

ABSTRACT
A new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient. It is considered unique because it is the first for an Iraqi population, and is expected to be a useful tool in breast cancer research. The AMJ13 cell line was established from the primary tumor of a 70-year-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. The cells were morphologically characterized by light and scanning electron microscopy, and found to be elongated multipolar epithelial-like cells with a population doubling time of 22 hours. The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature. Cytogenetic study of these cells showed chromosomal aberrations with many structural and numerical abnormalities, producing chromosomes of unknown origin called marker chromosomes. Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression. AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin. This cell line should be useful when testing new therapies for breast cancer in the Middle East.

No MeSH data available.


Related in: MedlinePlus

AMJ13 cell line morphology.Notes: (A) Morphology of AMJ13 cells in culture showing (B) an epithelial-like cell shape with multiple nuclei, a high N/C ratio, and the presence of mitotic figures (hematoxylin and eosin staining, magnification 40×). (C) Scanning electron micrographs of human breast cancer-derived cell cultures. The cells are squamous with many short and thin processes and grow upon each other (magnification 1,000×). (D) Scanning electron photomicrograph showing AMJ13 cells with characteristic epithelial polygonal shape (magnification 3,000×) (E) Scanning electron photomicrograph showing microvilli on the cancer cells that ranged from 0.792 µm to 5.98 µm in length (magnification 10,000×). (F) Scanning electron photomicrograph showing the measurements for AMJ13 cancer cells, for which the average length was 73.8 µm, the average width was 33 µm, and the average nucleus diameter was 11.1 µm (magnification 1,000×).
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f2-bctt-7-223: AMJ13 cell line morphology.Notes: (A) Morphology of AMJ13 cells in culture showing (B) an epithelial-like cell shape with multiple nuclei, a high N/C ratio, and the presence of mitotic figures (hematoxylin and eosin staining, magnification 40×). (C) Scanning electron micrographs of human breast cancer-derived cell cultures. The cells are squamous with many short and thin processes and grow upon each other (magnification 1,000×). (D) Scanning electron photomicrograph showing AMJ13 cells with characteristic epithelial polygonal shape (magnification 3,000×) (E) Scanning electron photomicrograph showing microvilli on the cancer cells that ranged from 0.792 µm to 5.98 µm in length (magnification 10,000×). (F) Scanning electron photomicrograph showing the measurements for AMJ13 cancer cells, for which the average length was 73.8 µm, the average width was 33 µm, and the average nucleus diameter was 11.1 µm (magnification 1,000×).

Mentions: The cultured AMJ13 cells had an elongated multipolar epithelial-like cell shape, with nuclear polymorphism and multiple nuclei in most of the cells, as well as showing many cells with mitotic figures, which expressed the characteristics of cell morphology (Figure 2A and B). Figure 2D is a scanning electron photomicrograph showing AMJ13 cells with a characteristic polygonal epithelial squamous shape with many short and thin processes, with cells growing upon each other. Measurements taken by scanning electron microscopy indicated a mean cell length of 73.8 µm, a mean cell width of 33 µm, and a mean nucleus diameter of 11.1 µm. Microvilli were also present, and ranged from 0.792 µm to 5.98 µm in length (Figure 2E).


Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient.

Al-Shammari AM, Alshami MA, Umran MA, Almukhtar AA, Yaseen NY, Raad K, Hussien AA - Breast Cancer (Dove Med Press) (2015)

AMJ13 cell line morphology.Notes: (A) Morphology of AMJ13 cells in culture showing (B) an epithelial-like cell shape with multiple nuclei, a high N/C ratio, and the presence of mitotic figures (hematoxylin and eosin staining, magnification 40×). (C) Scanning electron micrographs of human breast cancer-derived cell cultures. The cells are squamous with many short and thin processes and grow upon each other (magnification 1,000×). (D) Scanning electron photomicrograph showing AMJ13 cells with characteristic epithelial polygonal shape (magnification 3,000×) (E) Scanning electron photomicrograph showing microvilli on the cancer cells that ranged from 0.792 µm to 5.98 µm in length (magnification 10,000×). (F) Scanning electron photomicrograph showing the measurements for AMJ13 cancer cells, for which the average length was 73.8 µm, the average width was 33 µm, and the average nucleus diameter was 11.1 µm (magnification 1,000×).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4536763&req=5

f2-bctt-7-223: AMJ13 cell line morphology.Notes: (A) Morphology of AMJ13 cells in culture showing (B) an epithelial-like cell shape with multiple nuclei, a high N/C ratio, and the presence of mitotic figures (hematoxylin and eosin staining, magnification 40×). (C) Scanning electron micrographs of human breast cancer-derived cell cultures. The cells are squamous with many short and thin processes and grow upon each other (magnification 1,000×). (D) Scanning electron photomicrograph showing AMJ13 cells with characteristic epithelial polygonal shape (magnification 3,000×) (E) Scanning electron photomicrograph showing microvilli on the cancer cells that ranged from 0.792 µm to 5.98 µm in length (magnification 10,000×). (F) Scanning electron photomicrograph showing the measurements for AMJ13 cancer cells, for which the average length was 73.8 µm, the average width was 33 µm, and the average nucleus diameter was 11.1 µm (magnification 1,000×).
Mentions: The cultured AMJ13 cells had an elongated multipolar epithelial-like cell shape, with nuclear polymorphism and multiple nuclei in most of the cells, as well as showing many cells with mitotic figures, which expressed the characteristics of cell morphology (Figure 2A and B). Figure 2D is a scanning electron photomicrograph showing AMJ13 cells with a characteristic polygonal epithelial squamous shape with many short and thin processes, with cells growing upon each other. Measurements taken by scanning electron microscopy indicated a mean cell length of 73.8 µm, a mean cell width of 33 µm, and a mean nucleus diameter of 11.1 µm. Microvilli were also present, and ranged from 0.792 µm to 5.98 µm in length (Figure 2E).

Bottom Line: The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature.Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression.AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin.

View Article: PubMed Central - PubMed

Affiliation: Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, Baghdad, Iraq.

ABSTRACT
A new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient. It is considered unique because it is the first for an Iraqi population, and is expected to be a useful tool in breast cancer research. The AMJ13 cell line was established from the primary tumor of a 70-year-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. The cells were morphologically characterized by light and scanning electron microscopy, and found to be elongated multipolar epithelial-like cells with a population doubling time of 22 hours. The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature. Cytogenetic study of these cells showed chromosomal aberrations with many structural and numerical abnormalities, producing chromosomes of unknown origin called marker chromosomes. Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression. AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin. This cell line should be useful when testing new therapies for breast cancer in the Middle East.

No MeSH data available.


Related in: MedlinePlus