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Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient.

Al-Shammari AM, Alshami MA, Umran MA, Almukhtar AA, Yaseen NY, Raad K, Hussien AA - Breast Cancer (Dove Med Press) (2015)

Bottom Line: The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature.Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression.AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin.

View Article: PubMed Central - PubMed

Affiliation: Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, Baghdad, Iraq.

ABSTRACT
A new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient. It is considered unique because it is the first for an Iraqi population, and is expected to be a useful tool in breast cancer research. The AMJ13 cell line was established from the primary tumor of a 70-year-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. The cells were morphologically characterized by light and scanning electron microscopy, and found to be elongated multipolar epithelial-like cells with a population doubling time of 22 hours. The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature. Cytogenetic study of these cells showed chromosomal aberrations with many structural and numerical abnormalities, producing chromosomes of unknown origin called marker chromosomes. Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression. AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin. This cell line should be useful when testing new therapies for breast cancer in the Middle East.

No MeSH data available.


Related in: MedlinePlus

Histopathological sections of the original tumor of which the AMJ13 cell line was derived from.Notes: (A) A duct containing finger-like projections covered by a layer of epithelial and underlying myoepithelial cells with a fibrous core (hematoxylin and eosin staining, magnification 40×). (B) Infiltrating ductal carcinoma composed of irregular solid groups of cells in a dense fibrous stroma (hematoxylin and eosin staining, magnification 40×).
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f1-bctt-7-223: Histopathological sections of the original tumor of which the AMJ13 cell line was derived from.Notes: (A) A duct containing finger-like projections covered by a layer of epithelial and underlying myoepithelial cells with a fibrous core (hematoxylin and eosin staining, magnification 40×). (B) Infiltrating ductal carcinoma composed of irregular solid groups of cells in a dense fibrous stroma (hematoxylin and eosin staining, magnification 40×).

Mentions: The patient from whom this cell line derived was a 70-year-old female who lived in the city of Al-Ameen in Baghdad. She was 160 cm in height, weighed 70 kg, and had had six children. She had undergone a right-sided mastectomy for breast cancer, but had no genetic or chronic diseases. The histological sample of her breast mass that was used to established our cell line showed poorly differentiated infiltrative ductal carcinoma (Figure 1A and B), which was graded 3/3 according to the Nottingham modification of the Bloom–Richardson system, with no vascular invasion seen. The nipple and resection margins were free of malignancy. All axillary lymph nodes (n=11) were free of malignancy. According to the TNM classification, clinical TNM staging of this patient is T2 N0 Mx. Paraffin sections were positive for the ER and PR and positive for the Her/Neu2 tumor suppressor gene. The study was approved by Iraqi Center for Cancer and Medical, Genetic, and Scientific Institutional Review Board, and had received the patient consent for the use of patient-derived cell line.


Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient.

Al-Shammari AM, Alshami MA, Umran MA, Almukhtar AA, Yaseen NY, Raad K, Hussien AA - Breast Cancer (Dove Med Press) (2015)

Histopathological sections of the original tumor of which the AMJ13 cell line was derived from.Notes: (A) A duct containing finger-like projections covered by a layer of epithelial and underlying myoepithelial cells with a fibrous core (hematoxylin and eosin staining, magnification 40×). (B) Infiltrating ductal carcinoma composed of irregular solid groups of cells in a dense fibrous stroma (hematoxylin and eosin staining, magnification 40×).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4536763&req=5

f1-bctt-7-223: Histopathological sections of the original tumor of which the AMJ13 cell line was derived from.Notes: (A) A duct containing finger-like projections covered by a layer of epithelial and underlying myoepithelial cells with a fibrous core (hematoxylin and eosin staining, magnification 40×). (B) Infiltrating ductal carcinoma composed of irregular solid groups of cells in a dense fibrous stroma (hematoxylin and eosin staining, magnification 40×).
Mentions: The patient from whom this cell line derived was a 70-year-old female who lived in the city of Al-Ameen in Baghdad. She was 160 cm in height, weighed 70 kg, and had had six children. She had undergone a right-sided mastectomy for breast cancer, but had no genetic or chronic diseases. The histological sample of her breast mass that was used to established our cell line showed poorly differentiated infiltrative ductal carcinoma (Figure 1A and B), which was graded 3/3 according to the Nottingham modification of the Bloom–Richardson system, with no vascular invasion seen. The nipple and resection margins were free of malignancy. All axillary lymph nodes (n=11) were free of malignancy. According to the TNM classification, clinical TNM staging of this patient is T2 N0 Mx. Paraffin sections were positive for the ER and PR and positive for the Her/Neu2 tumor suppressor gene. The study was approved by Iraqi Center for Cancer and Medical, Genetic, and Scientific Institutional Review Board, and had received the patient consent for the use of patient-derived cell line.

Bottom Line: The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature.Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression.AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin.

View Article: PubMed Central - PubMed

Affiliation: Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, Baghdad, Iraq.

ABSTRACT
A new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient. It is considered unique because it is the first for an Iraqi population, and is expected to be a useful tool in breast cancer research. The AMJ13 cell line was established from the primary tumor of a 70-year-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. The cells were morphologically characterized by light and scanning electron microscopy, and found to be elongated multipolar epithelial-like cells with a population doubling time of 22 hours. The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature. Cytogenetic study of these cells showed chromosomal aberrations with many structural and numerical abnormalities, producing chromosomes of unknown origin called marker chromosomes. Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression. AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin. This cell line should be useful when testing new therapies for breast cancer in the Middle East.

No MeSH data available.


Related in: MedlinePlus