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The major acute phase proteins of bovine milk in a commercial dairy herd.

Thomas FC, Waterston M, Hastie P, Parkin T, Haining H, Eckersall PD - BMC Vet. Res. (2015)

Bottom Line: Composite milk APP results were compared with cow factors including parity, stage of lactation, percentage protein and fat as well as somatic cell counts (SCC).Significant correlations were found between composite SCC and Hp (P-value <0.009) as well as parity and Hp (P < 0.009), but not between M-SAA3 and SCC, M-SAA3 and Hp, M-SAA3 and CRP or M-SAA3 and parity.The lack of correlation of SCC with the M-SAA3 and CRP could result from these APP being more sensitive to intra-mammary infection than SCC.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biodiversity, Animal Health and Comparative Medicine, College of Veterinary Medical and Life Sciences, University of Glasgow, Bearsden Rd, Glasgow, G61 1QH, UK. f.thomas.1@research.gla.ac.uk.

ABSTRACT

Background: Milk acute phase proteins (APP) have been identified and show promise as biomarkers of mastitis. However analysis of their profile in dairy cows from a production herd is necessary in order to confirm their benefits in mastitis diagnosis. The profiles of milk haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) were determined in 54 composite milk (milk from all functional quarters of a cow's udder collected in a common receptacle) samples (CMS) from a commercial dairy farm. Milk Hp was also determined in individual quarter milk (milk from a single udder quarter) samples (QMS) (n = 149) of the cows. An ELISA was developed and validated for the determination of milk Hp while commercial kits were used for M-SAA3 and CRP assay respectively. Composite milk APP results were compared with cow factors including parity, stage of lactation, percentage protein and fat as well as somatic cell counts (SCC).

Results: Composite milk Hp ranged from <0.4-55 μg/ml with a median of 3.5 μg/ml; composite milk M-SAA3 ranged from <0.6-50 μg/ml and had a median of 1.2 μg/ml, while CRP ranged from <1.80-173 ng/ml and had a median of 24.6 ng/ml. Significant correlations were found between composite SCC and Hp (P-value <0.009) as well as parity and Hp (P < 0.009), but not between M-SAA3 and SCC, M-SAA3 and Hp, M-SAA3 and CRP or M-SAA3 and parity. Milk CRP was correlated with % fat (P = 0.002) and % protein (P = 0.001) of the milk samples. The lack of correlation of SCC with the M-SAA3 and CRP could result from these APP being more sensitive to intra-mammary infection than SCC. Quarter milk Hp had a range of <0.4-420 μg/ml with a median value of 3.6 μg/ml, with 92 % of samples below 20 μg/ml.

Conclusion: Baseline values of Hp, M-SAA3 and CRP were established in composite milk from cows with normal SCC on the dairy farm. Parity was recognized as a possible confounding factor when diagnosing mastitis using Hp. The value of the APP, Hp, M-SAA3 and CRP as substitutes or to complement SCC in indicating udder inflammation, was demonstrated.

No MeSH data available.


Related in: MedlinePlus

Distribution of milk Hp concentration in quarter milk samples (n = 149). Median-3.60 μg/ml, range <0.4–420 μg/ml
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Fig4: Distribution of milk Hp concentration in quarter milk samples (n = 149). Median-3.60 μg/ml, range <0.4–420 μg/ml

Mentions: Haptoglobin from individual QMS from cows in the herd were not normally distributed with a higher percentage of samples falling within the low Hp category, Hp concentration in QMS ranged from <0.4–420 μg/ml with a median of 3.6 μg/ml, shown in Fig. 4. The higher maximum concentration of quarter milk samples than the composite samples highlights the effect of dilution by milk of non-infected quarters on infected quarters’ parameters which has been reported by several authors for example for SCC by Åkerstedt et al. [3]. It would therefore be in the interest of greater accuracy of detecting IMI and infected quarters to use quarter-milk samples for assay of APP.Fig. 4


The major acute phase proteins of bovine milk in a commercial dairy herd.

Thomas FC, Waterston M, Hastie P, Parkin T, Haining H, Eckersall PD - BMC Vet. Res. (2015)

Distribution of milk Hp concentration in quarter milk samples (n = 149). Median-3.60 μg/ml, range <0.4–420 μg/ml
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536752&req=5

Fig4: Distribution of milk Hp concentration in quarter milk samples (n = 149). Median-3.60 μg/ml, range <0.4–420 μg/ml
Mentions: Haptoglobin from individual QMS from cows in the herd were not normally distributed with a higher percentage of samples falling within the low Hp category, Hp concentration in QMS ranged from <0.4–420 μg/ml with a median of 3.6 μg/ml, shown in Fig. 4. The higher maximum concentration of quarter milk samples than the composite samples highlights the effect of dilution by milk of non-infected quarters on infected quarters’ parameters which has been reported by several authors for example for SCC by Åkerstedt et al. [3]. It would therefore be in the interest of greater accuracy of detecting IMI and infected quarters to use quarter-milk samples for assay of APP.Fig. 4

Bottom Line: Composite milk APP results were compared with cow factors including parity, stage of lactation, percentage protein and fat as well as somatic cell counts (SCC).Significant correlations were found between composite SCC and Hp (P-value <0.009) as well as parity and Hp (P < 0.009), but not between M-SAA3 and SCC, M-SAA3 and Hp, M-SAA3 and CRP or M-SAA3 and parity.The lack of correlation of SCC with the M-SAA3 and CRP could result from these APP being more sensitive to intra-mammary infection than SCC.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biodiversity, Animal Health and Comparative Medicine, College of Veterinary Medical and Life Sciences, University of Glasgow, Bearsden Rd, Glasgow, G61 1QH, UK. f.thomas.1@research.gla.ac.uk.

ABSTRACT

Background: Milk acute phase proteins (APP) have been identified and show promise as biomarkers of mastitis. However analysis of their profile in dairy cows from a production herd is necessary in order to confirm their benefits in mastitis diagnosis. The profiles of milk haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) were determined in 54 composite milk (milk from all functional quarters of a cow's udder collected in a common receptacle) samples (CMS) from a commercial dairy farm. Milk Hp was also determined in individual quarter milk (milk from a single udder quarter) samples (QMS) (n = 149) of the cows. An ELISA was developed and validated for the determination of milk Hp while commercial kits were used for M-SAA3 and CRP assay respectively. Composite milk APP results were compared with cow factors including parity, stage of lactation, percentage protein and fat as well as somatic cell counts (SCC).

Results: Composite milk Hp ranged from <0.4-55 μg/ml with a median of 3.5 μg/ml; composite milk M-SAA3 ranged from <0.6-50 μg/ml and had a median of 1.2 μg/ml, while CRP ranged from <1.80-173 ng/ml and had a median of 24.6 ng/ml. Significant correlations were found between composite SCC and Hp (P-value <0.009) as well as parity and Hp (P < 0.009), but not between M-SAA3 and SCC, M-SAA3 and Hp, M-SAA3 and CRP or M-SAA3 and parity. Milk CRP was correlated with % fat (P = 0.002) and % protein (P = 0.001) of the milk samples. The lack of correlation of SCC with the M-SAA3 and CRP could result from these APP being more sensitive to intra-mammary infection than SCC. Quarter milk Hp had a range of <0.4-420 μg/ml with a median value of 3.6 μg/ml, with 92 % of samples below 20 μg/ml.

Conclusion: Baseline values of Hp, M-SAA3 and CRP were established in composite milk from cows with normal SCC on the dairy farm. Parity was recognized as a possible confounding factor when diagnosing mastitis using Hp. The value of the APP, Hp, M-SAA3 and CRP as substitutes or to complement SCC in indicating udder inflammation, was demonstrated.

No MeSH data available.


Related in: MedlinePlus