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Effects of the aqueous extract of a Tibetan herb, Rhodiola algida var. tangutica on proliferation and HIF-1α, HIF-2α expression in MCF-7 cells under hypoxic condition in vitro.

Qi YJ, Cui S, Lu DX, Yang YZ, Luo Y, Ma L, Ma Y, Wuren T, Chang R, Qi L, Ben BJ, Han J, Ge RL - Cancer Cell Int. (2015)

Bottom Line: Rhodiola algida var. tangutica is a traditional Tibetan herb.Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness.The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction.

View Article: PubMed Central - PubMed

Affiliation: Qinghai Province people's Hospital, Xining, 810007 Qinghai Peoples' Republic of China ; Research Center for High Altitude Medicine in Qinghai University, 16 Kunlun Road, Xining, 810001 Qinghai Peoples' Republic of China.

ABSTRACT

Ethnopharmacological relevance: Rhodiola algida var. tangutica is a traditional Tibetan herb. Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness. This study aimed to investigate the effect of Rhodiola algida var. tangutica on hypoxic MCF-7 breast cancer cells and the underlying mechanisms.

Materials and methods: The antiproliferative effects of R. algida on MCF-7 breast cancer cells were compared in vitro under hypoxic and normal conditions by using MTT analysis. The influence of R. algida on cancer cell apoptosis was determined by flow cytometry. The expression levels of hypoxia-inducible factor (HIF)-1α and HIF-2α were evaluated by western blot analysis.

Results: R. algida inhibited the proliferation of MCF-7 breast cancer cells in a dose- and time-dependent manner. The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction. Pretreatment with R. algida significantly suppressed the hypoxia-induced proliferation and expression of HIF-1α and HIF-2α in MCF-7 breast cancer cells.

Conclusions: R. algida might exert an anti-carcinogenic effect on MCF-7 breast cancer cells by decreasing the protein levels of HIF-1α and HIF-2α, which are overexpressed under hypoxic conditions. This effect might be elicited by inhibiting the hypoxia-induced proliferation of MCF-7 breast cancer cells.

No MeSH data available.


Related in: MedlinePlus

Effects of hypoxia and Rhodiola algida var. tangutica on HIF-1α, HIF-2α protein expression in MCF-7 cells cultured detected by Western blotting. a Western blots of cultured MCF-7 breast cancer cells exposed to hypoxia for 48 h in the absence or presence of various concentrations of Rhodiola algida var. tangutica (45, 90, 180, 225, 360 μg/mL). Cell lysates were analyzed by Western blotting with antibodies as indicated above. GAPDH protein expression was used as a control. b, cBars show the relative expression levels of HIF-1α, HIF-2α (optical density of HIF-1α, HIF-2α normalized against GAPDH expression). Data are mean ± SEM of three identical experiments. N48 h indicates exposure to normoxia for 48 h; H48 h, exposure to hypoxia for 48 h. ◆P < 0.01 vs. N48 h group, ★P < 0.05 vs. H48 h group.
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Fig5: Effects of hypoxia and Rhodiola algida var. tangutica on HIF-1α, HIF-2α protein expression in MCF-7 cells cultured detected by Western blotting. a Western blots of cultured MCF-7 breast cancer cells exposed to hypoxia for 48 h in the absence or presence of various concentrations of Rhodiola algida var. tangutica (45, 90, 180, 225, 360 μg/mL). Cell lysates were analyzed by Western blotting with antibodies as indicated above. GAPDH protein expression was used as a control. b, cBars show the relative expression levels of HIF-1α, HIF-2α (optical density of HIF-1α, HIF-2α normalized against GAPDH expression). Data are mean ± SEM of three identical experiments. N48 h indicates exposure to normoxia for 48 h; H48 h, exposure to hypoxia for 48 h. ◆P < 0.01 vs. N48 h group, ★P < 0.05 vs. H48 h group.

Mentions: Hypoxia increased the expressionof HIF-1α and HIF-2α in MCF-7 breast cancer cells, whereas treatment with R. algida extract (225 and 360 μg/mL) possibly decreased HIF-1α and HIF-2α protein expression in cells exposed to hypoxia for 48 h (Fig. 5, a–c).Fig. 5


Effects of the aqueous extract of a Tibetan herb, Rhodiola algida var. tangutica on proliferation and HIF-1α, HIF-2α expression in MCF-7 cells under hypoxic condition in vitro.

Qi YJ, Cui S, Lu DX, Yang YZ, Luo Y, Ma L, Ma Y, Wuren T, Chang R, Qi L, Ben BJ, Han J, Ge RL - Cancer Cell Int. (2015)

Effects of hypoxia and Rhodiola algida var. tangutica on HIF-1α, HIF-2α protein expression in MCF-7 cells cultured detected by Western blotting. a Western blots of cultured MCF-7 breast cancer cells exposed to hypoxia for 48 h in the absence or presence of various concentrations of Rhodiola algida var. tangutica (45, 90, 180, 225, 360 μg/mL). Cell lysates were analyzed by Western blotting with antibodies as indicated above. GAPDH protein expression was used as a control. b, cBars show the relative expression levels of HIF-1α, HIF-2α (optical density of HIF-1α, HIF-2α normalized against GAPDH expression). Data are mean ± SEM of three identical experiments. N48 h indicates exposure to normoxia for 48 h; H48 h, exposure to hypoxia for 48 h. ◆P < 0.01 vs. N48 h group, ★P < 0.05 vs. H48 h group.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4536750&req=5

Fig5: Effects of hypoxia and Rhodiola algida var. tangutica on HIF-1α, HIF-2α protein expression in MCF-7 cells cultured detected by Western blotting. a Western blots of cultured MCF-7 breast cancer cells exposed to hypoxia for 48 h in the absence or presence of various concentrations of Rhodiola algida var. tangutica (45, 90, 180, 225, 360 μg/mL). Cell lysates were analyzed by Western blotting with antibodies as indicated above. GAPDH protein expression was used as a control. b, cBars show the relative expression levels of HIF-1α, HIF-2α (optical density of HIF-1α, HIF-2α normalized against GAPDH expression). Data are mean ± SEM of three identical experiments. N48 h indicates exposure to normoxia for 48 h; H48 h, exposure to hypoxia for 48 h. ◆P < 0.01 vs. N48 h group, ★P < 0.05 vs. H48 h group.
Mentions: Hypoxia increased the expressionof HIF-1α and HIF-2α in MCF-7 breast cancer cells, whereas treatment with R. algida extract (225 and 360 μg/mL) possibly decreased HIF-1α and HIF-2α protein expression in cells exposed to hypoxia for 48 h (Fig. 5, a–c).Fig. 5

Bottom Line: Rhodiola algida var. tangutica is a traditional Tibetan herb.Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness.The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction.

View Article: PubMed Central - PubMed

Affiliation: Qinghai Province people's Hospital, Xining, 810007 Qinghai Peoples' Republic of China ; Research Center for High Altitude Medicine in Qinghai University, 16 Kunlun Road, Xining, 810001 Qinghai Peoples' Republic of China.

ABSTRACT

Ethnopharmacological relevance: Rhodiola algida var. tangutica is a traditional Tibetan herb. Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness. This study aimed to investigate the effect of Rhodiola algida var. tangutica on hypoxic MCF-7 breast cancer cells and the underlying mechanisms.

Materials and methods: The antiproliferative effects of R. algida on MCF-7 breast cancer cells were compared in vitro under hypoxic and normal conditions by using MTT analysis. The influence of R. algida on cancer cell apoptosis was determined by flow cytometry. The expression levels of hypoxia-inducible factor (HIF)-1α and HIF-2α were evaluated by western blot analysis.

Results: R. algida inhibited the proliferation of MCF-7 breast cancer cells in a dose- and time-dependent manner. The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction. Pretreatment with R. algida significantly suppressed the hypoxia-induced proliferation and expression of HIF-1α and HIF-2α in MCF-7 breast cancer cells.

Conclusions: R. algida might exert an anti-carcinogenic effect on MCF-7 breast cancer cells by decreasing the protein levels of HIF-1α and HIF-2α, which are overexpressed under hypoxic conditions. This effect might be elicited by inhibiting the hypoxia-induced proliferation of MCF-7 breast cancer cells.

No MeSH data available.


Related in: MedlinePlus