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Effects of the aqueous extract of a Tibetan herb, Rhodiola algida var. tangutica on proliferation and HIF-1α, HIF-2α expression in MCF-7 cells under hypoxic condition in vitro.

Qi YJ, Cui S, Lu DX, Yang YZ, Luo Y, Ma L, Ma Y, Wuren T, Chang R, Qi L, Ben BJ, Han J, Ge RL - Cancer Cell Int. (2015)

Bottom Line: Rhodiola algida var. tangutica is a traditional Tibetan herb.Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness.The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction.

View Article: PubMed Central - PubMed

Affiliation: Qinghai Province people's Hospital, Xining, 810007 Qinghai Peoples' Republic of China ; Research Center for High Altitude Medicine in Qinghai University, 16 Kunlun Road, Xining, 810001 Qinghai Peoples' Republic of China.

ABSTRACT

Ethnopharmacological relevance: Rhodiola algida var. tangutica is a traditional Tibetan herb. Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness. This study aimed to investigate the effect of Rhodiola algida var. tangutica on hypoxic MCF-7 breast cancer cells and the underlying mechanisms.

Materials and methods: The antiproliferative effects of R. algida on MCF-7 breast cancer cells were compared in vitro under hypoxic and normal conditions by using MTT analysis. The influence of R. algida on cancer cell apoptosis was determined by flow cytometry. The expression levels of hypoxia-inducible factor (HIF)-1α and HIF-2α were evaluated by western blot analysis.

Results: R. algida inhibited the proliferation of MCF-7 breast cancer cells in a dose- and time-dependent manner. The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction. Pretreatment with R. algida significantly suppressed the hypoxia-induced proliferation and expression of HIF-1α and HIF-2α in MCF-7 breast cancer cells.

Conclusions: R. algida might exert an anti-carcinogenic effect on MCF-7 breast cancer cells by decreasing the protein levels of HIF-1α and HIF-2α, which are overexpressed under hypoxic conditions. This effect might be elicited by inhibiting the hypoxia-induced proliferation of MCF-7 breast cancer cells.

No MeSH data available.


Related in: MedlinePlus

Effect of Rhodiola algida var. tangutica on proliferation of MCF-7 breast cancer cells exposed to normoxia and hypoxia for 48 h by direct cell counting. Serum-starved MCF-7 breast cancer cells exposed to normoxia and hypoxia were incubated with Rhodiola algida var. tangutica at different concentrations (0, 45, 90, 180, 225, 360 μg/mL) for 48 h. And then the cell counts were determined with a hematocytometer. Values are expressed as percentage of untreated cells, and each bar represents the mean ± S.D. of triplicate determinations. N48 h indicates exposure to normoxia for 48 h; H48 h, exposure to hypoxia for 48 h. ※P < 0.05 vs. N48 h in the absence of Rhodiola algida var. tangutica. ▲P < 0.01 vs. N48 h in the absence of Rhodiola algida var. tangutica; ★P < 0.05 vs. H48 h in the absence of Rhodiola algida var. tangutica.
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Fig4: Effect of Rhodiola algida var. tangutica on proliferation of MCF-7 breast cancer cells exposed to normoxia and hypoxia for 48 h by direct cell counting. Serum-starved MCF-7 breast cancer cells exposed to normoxia and hypoxia were incubated with Rhodiola algida var. tangutica at different concentrations (0, 45, 90, 180, 225, 360 μg/mL) for 48 h. And then the cell counts were determined with a hematocytometer. Values are expressed as percentage of untreated cells, and each bar represents the mean ± S.D. of triplicate determinations. N48 h indicates exposure to normoxia for 48 h; H48 h, exposure to hypoxia for 48 h. ※P < 0.05 vs. N48 h in the absence of Rhodiola algida var. tangutica. ▲P < 0.01 vs. N48 h in the absence of Rhodiola algida var. tangutica; ★P < 0.05 vs. H48 h in the absence of Rhodiola algida var. tangutica.

Mentions: Evaluation of whether R. algida extract affected the proliferation of MCF-7 cells induced by hypoxia was performed using the MTT assay and direct cell counting for nonradioactive quantification of cell proliferation. The results showed that R. algida extract inhibited the hypoxia-induced proliferation of MCF-7 cells in a concentration-dependent manner. Significantly reduced cell proliferation of treated cells was also noted under normoxic condition, although at lower concentrations of R. algida extract (180 and 225 μg/mL; Table 2). Similar results were obtained by direct cell counting (Fig. 4).Table 2


Effects of the aqueous extract of a Tibetan herb, Rhodiola algida var. tangutica on proliferation and HIF-1α, HIF-2α expression in MCF-7 cells under hypoxic condition in vitro.

Qi YJ, Cui S, Lu DX, Yang YZ, Luo Y, Ma L, Ma Y, Wuren T, Chang R, Qi L, Ben BJ, Han J, Ge RL - Cancer Cell Int. (2015)

Effect of Rhodiola algida var. tangutica on proliferation of MCF-7 breast cancer cells exposed to normoxia and hypoxia for 48 h by direct cell counting. Serum-starved MCF-7 breast cancer cells exposed to normoxia and hypoxia were incubated with Rhodiola algida var. tangutica at different concentrations (0, 45, 90, 180, 225, 360 μg/mL) for 48 h. And then the cell counts were determined with a hematocytometer. Values are expressed as percentage of untreated cells, and each bar represents the mean ± S.D. of triplicate determinations. N48 h indicates exposure to normoxia for 48 h; H48 h, exposure to hypoxia for 48 h. ※P < 0.05 vs. N48 h in the absence of Rhodiola algida var. tangutica. ▲P < 0.01 vs. N48 h in the absence of Rhodiola algida var. tangutica; ★P < 0.05 vs. H48 h in the absence of Rhodiola algida var. tangutica.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536750&req=5

Fig4: Effect of Rhodiola algida var. tangutica on proliferation of MCF-7 breast cancer cells exposed to normoxia and hypoxia for 48 h by direct cell counting. Serum-starved MCF-7 breast cancer cells exposed to normoxia and hypoxia were incubated with Rhodiola algida var. tangutica at different concentrations (0, 45, 90, 180, 225, 360 μg/mL) for 48 h. And then the cell counts were determined with a hematocytometer. Values are expressed as percentage of untreated cells, and each bar represents the mean ± S.D. of triplicate determinations. N48 h indicates exposure to normoxia for 48 h; H48 h, exposure to hypoxia for 48 h. ※P < 0.05 vs. N48 h in the absence of Rhodiola algida var. tangutica. ▲P < 0.01 vs. N48 h in the absence of Rhodiola algida var. tangutica; ★P < 0.05 vs. H48 h in the absence of Rhodiola algida var. tangutica.
Mentions: Evaluation of whether R. algida extract affected the proliferation of MCF-7 cells induced by hypoxia was performed using the MTT assay and direct cell counting for nonradioactive quantification of cell proliferation. The results showed that R. algida extract inhibited the hypoxia-induced proliferation of MCF-7 cells in a concentration-dependent manner. Significantly reduced cell proliferation of treated cells was also noted under normoxic condition, although at lower concentrations of R. algida extract (180 and 225 μg/mL; Table 2). Similar results were obtained by direct cell counting (Fig. 4).Table 2

Bottom Line: Rhodiola algida var. tangutica is a traditional Tibetan herb.Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness.The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction.

View Article: PubMed Central - PubMed

Affiliation: Qinghai Province people's Hospital, Xining, 810007 Qinghai Peoples' Republic of China ; Research Center for High Altitude Medicine in Qinghai University, 16 Kunlun Road, Xining, 810001 Qinghai Peoples' Republic of China.

ABSTRACT

Ethnopharmacological relevance: Rhodiola algida var. tangutica is a traditional Tibetan herb. Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness. This study aimed to investigate the effect of Rhodiola algida var. tangutica on hypoxic MCF-7 breast cancer cells and the underlying mechanisms.

Materials and methods: The antiproliferative effects of R. algida on MCF-7 breast cancer cells were compared in vitro under hypoxic and normal conditions by using MTT analysis. The influence of R. algida on cancer cell apoptosis was determined by flow cytometry. The expression levels of hypoxia-inducible factor (HIF)-1α and HIF-2α were evaluated by western blot analysis.

Results: R. algida inhibited the proliferation of MCF-7 breast cancer cells in a dose- and time-dependent manner. The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction. Pretreatment with R. algida significantly suppressed the hypoxia-induced proliferation and expression of HIF-1α and HIF-2α in MCF-7 breast cancer cells.

Conclusions: R. algida might exert an anti-carcinogenic effect on MCF-7 breast cancer cells by decreasing the protein levels of HIF-1α and HIF-2α, which are overexpressed under hypoxic conditions. This effect might be elicited by inhibiting the hypoxia-induced proliferation of MCF-7 breast cancer cells.

No MeSH data available.


Related in: MedlinePlus