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Effects of the aqueous extract of a Tibetan herb, Rhodiola algida var. tangutica on proliferation and HIF-1α, HIF-2α expression in MCF-7 cells under hypoxic condition in vitro.

Qi YJ, Cui S, Lu DX, Yang YZ, Luo Y, Ma L, Ma Y, Wuren T, Chang R, Qi L, Ben BJ, Han J, Ge RL - Cancer Cell Int. (2015)

Bottom Line: Rhodiola algida var. tangutica is a traditional Tibetan herb.Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness.The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction.

View Article: PubMed Central - PubMed

Affiliation: Qinghai Province people's Hospital, Xining, 810007 Qinghai Peoples' Republic of China ; Research Center for High Altitude Medicine in Qinghai University, 16 Kunlun Road, Xining, 810001 Qinghai Peoples' Republic of China.

ABSTRACT

Ethnopharmacological relevance: Rhodiola algida var. tangutica is a traditional Tibetan herb. Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness. This study aimed to investigate the effect of Rhodiola algida var. tangutica on hypoxic MCF-7 breast cancer cells and the underlying mechanisms.

Materials and methods: The antiproliferative effects of R. algida on MCF-7 breast cancer cells were compared in vitro under hypoxic and normal conditions by using MTT analysis. The influence of R. algida on cancer cell apoptosis was determined by flow cytometry. The expression levels of hypoxia-inducible factor (HIF)-1α and HIF-2α were evaluated by western blot analysis.

Results: R. algida inhibited the proliferation of MCF-7 breast cancer cells in a dose- and time-dependent manner. The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction. Pretreatment with R. algida significantly suppressed the hypoxia-induced proliferation and expression of HIF-1α and HIF-2α in MCF-7 breast cancer cells.

Conclusions: R. algida might exert an anti-carcinogenic effect on MCF-7 breast cancer cells by decreasing the protein levels of HIF-1α and HIF-2α, which are overexpressed under hypoxic conditions. This effect might be elicited by inhibiting the hypoxia-induced proliferation of MCF-7 breast cancer cells.

No MeSH data available.


Related in: MedlinePlus

Induction of apoptosis and/or necrosis assessed by flow cytometry. Externalization of phosphotidylserine (PS) to the outer leaflet from the inner leaflet of the plasma membrane is a hallmark of early apoptosis. The FITC labeled Annexin V binds to PS in presence of calcium ions, resulting in green fluorescence of apoptotic cells. In later stages of apoptosis or necrosis, PI enters the cells and bind to cellular DNA, resulting in red and intense green fluorescence with Annexin V. As shown in the Fig. 3, cells treated with the lower concentration showed the present of Annexin V (+) (lower right quadrant) indicating the induction of early apoptosis. However, at higher concentration, there was a prominent rise of Annexin V(+)/PI (+) indicative of late apoptosis/necrosis.
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Fig3: Induction of apoptosis and/or necrosis assessed by flow cytometry. Externalization of phosphotidylserine (PS) to the outer leaflet from the inner leaflet of the plasma membrane is a hallmark of early apoptosis. The FITC labeled Annexin V binds to PS in presence of calcium ions, resulting in green fluorescence of apoptotic cells. In later stages of apoptosis or necrosis, PI enters the cells and bind to cellular DNA, resulting in red and intense green fluorescence with Annexin V. As shown in the Fig. 3, cells treated with the lower concentration showed the present of Annexin V (+) (lower right quadrant) indicating the induction of early apoptosis. However, at higher concentration, there was a prominent rise of Annexin V(+)/PI (+) indicative of late apoptosis/necrosis.

Mentions: The percentage of MCF-7 cells increased under the hypoxic condition, but this increase in cell proliferation was markedly decreased by treatment with R. algida extract (Fig. 3). The data represented mean ± S.D. (n = 15). P < 0.05 vs. normoxia. This suggested that R. algida var. tangutica induced apoptosis under hypoxia in MCF-7 breast cancer cells.Fig. 3


Effects of the aqueous extract of a Tibetan herb, Rhodiola algida var. tangutica on proliferation and HIF-1α, HIF-2α expression in MCF-7 cells under hypoxic condition in vitro.

Qi YJ, Cui S, Lu DX, Yang YZ, Luo Y, Ma L, Ma Y, Wuren T, Chang R, Qi L, Ben BJ, Han J, Ge RL - Cancer Cell Int. (2015)

Induction of apoptosis and/or necrosis assessed by flow cytometry. Externalization of phosphotidylserine (PS) to the outer leaflet from the inner leaflet of the plasma membrane is a hallmark of early apoptosis. The FITC labeled Annexin V binds to PS in presence of calcium ions, resulting in green fluorescence of apoptotic cells. In later stages of apoptosis or necrosis, PI enters the cells and bind to cellular DNA, resulting in red and intense green fluorescence with Annexin V. As shown in the Fig. 3, cells treated with the lower concentration showed the present of Annexin V (+) (lower right quadrant) indicating the induction of early apoptosis. However, at higher concentration, there was a prominent rise of Annexin V(+)/PI (+) indicative of late apoptosis/necrosis.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536750&req=5

Fig3: Induction of apoptosis and/or necrosis assessed by flow cytometry. Externalization of phosphotidylserine (PS) to the outer leaflet from the inner leaflet of the plasma membrane is a hallmark of early apoptosis. The FITC labeled Annexin V binds to PS in presence of calcium ions, resulting in green fluorescence of apoptotic cells. In later stages of apoptosis or necrosis, PI enters the cells and bind to cellular DNA, resulting in red and intense green fluorescence with Annexin V. As shown in the Fig. 3, cells treated with the lower concentration showed the present of Annexin V (+) (lower right quadrant) indicating the induction of early apoptosis. However, at higher concentration, there was a prominent rise of Annexin V(+)/PI (+) indicative of late apoptosis/necrosis.
Mentions: The percentage of MCF-7 cells increased under the hypoxic condition, but this increase in cell proliferation was markedly decreased by treatment with R. algida extract (Fig. 3). The data represented mean ± S.D. (n = 15). P < 0.05 vs. normoxia. This suggested that R. algida var. tangutica induced apoptosis under hypoxia in MCF-7 breast cancer cells.Fig. 3

Bottom Line: Rhodiola algida var. tangutica is a traditional Tibetan herb.Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness.The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction.

View Article: PubMed Central - PubMed

Affiliation: Qinghai Province people's Hospital, Xining, 810007 Qinghai Peoples' Republic of China ; Research Center for High Altitude Medicine in Qinghai University, 16 Kunlun Road, Xining, 810001 Qinghai Peoples' Republic of China.

ABSTRACT

Ethnopharmacological relevance: Rhodiola algida var. tangutica is a traditional Tibetan herb. Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness. This study aimed to investigate the effect of Rhodiola algida var. tangutica on hypoxic MCF-7 breast cancer cells and the underlying mechanisms.

Materials and methods: The antiproliferative effects of R. algida on MCF-7 breast cancer cells were compared in vitro under hypoxic and normal conditions by using MTT analysis. The influence of R. algida on cancer cell apoptosis was determined by flow cytometry. The expression levels of hypoxia-inducible factor (HIF)-1α and HIF-2α were evaluated by western blot analysis.

Results: R. algida inhibited the proliferation of MCF-7 breast cancer cells in a dose- and time-dependent manner. The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction. Pretreatment with R. algida significantly suppressed the hypoxia-induced proliferation and expression of HIF-1α and HIF-2α in MCF-7 breast cancer cells.

Conclusions: R. algida might exert an anti-carcinogenic effect on MCF-7 breast cancer cells by decreasing the protein levels of HIF-1α and HIF-2α, which are overexpressed under hypoxic conditions. This effect might be elicited by inhibiting the hypoxia-induced proliferation of MCF-7 breast cancer cells.

No MeSH data available.


Related in: MedlinePlus