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Effects of the aqueous extract of a Tibetan herb, Rhodiola algida var. tangutica on proliferation and HIF-1α, HIF-2α expression in MCF-7 cells under hypoxic condition in vitro.

Qi YJ, Cui S, Lu DX, Yang YZ, Luo Y, Ma L, Ma Y, Wuren T, Chang R, Qi L, Ben BJ, Han J, Ge RL - Cancer Cell Int. (2015)

Bottom Line: Rhodiola algida var. tangutica is a traditional Tibetan herb.Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness.The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction.

View Article: PubMed Central - PubMed

Affiliation: Qinghai Province people's Hospital, Xining, 810007 Qinghai Peoples' Republic of China ; Research Center for High Altitude Medicine in Qinghai University, 16 Kunlun Road, Xining, 810001 Qinghai Peoples' Republic of China.

ABSTRACT

Ethnopharmacological relevance: Rhodiola algida var. tangutica is a traditional Tibetan herb. Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness. This study aimed to investigate the effect of Rhodiola algida var. tangutica on hypoxic MCF-7 breast cancer cells and the underlying mechanisms.

Materials and methods: The antiproliferative effects of R. algida on MCF-7 breast cancer cells were compared in vitro under hypoxic and normal conditions by using MTT analysis. The influence of R. algida on cancer cell apoptosis was determined by flow cytometry. The expression levels of hypoxia-inducible factor (HIF)-1α and HIF-2α were evaluated by western blot analysis.

Results: R. algida inhibited the proliferation of MCF-7 breast cancer cells in a dose- and time-dependent manner. The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction. Pretreatment with R. algida significantly suppressed the hypoxia-induced proliferation and expression of HIF-1α and HIF-2α in MCF-7 breast cancer cells.

Conclusions: R. algida might exert an anti-carcinogenic effect on MCF-7 breast cancer cells by decreasing the protein levels of HIF-1α and HIF-2α, which are overexpressed under hypoxic conditions. This effect might be elicited by inhibiting the hypoxia-induced proliferation of MCF-7 breast cancer cells.

No MeSH data available.


Related in: MedlinePlus

Rhodiola algida var. tangutica inhibits hypoxia-induced proliferation in MCF-7 cells. The cells were plated at a seeding density of 5 × 104 viable cells/cm2 and grown as a monolayer in a 37°C incubator with a humidified atmosphere of 5% CO2 in a 2.0% O2 or normoxic. The images were obtained using 20× objectives 48 h after plating. a Morphology from normal MCF-7 breast cancer cells in adherent culture exposed to normoxic 48 h (N48h). b Morphology from MCF-7 breast cancer cells in adherent culture exposed to hypoxia 48 h (H48h), showing proliferation after exposure to hypoxia. c Morphology from MCF-7 breast cancer cells in adherent culture receiving Rhodiola algida var. tangutica (360 μg/mL) for 48 h under normoxic condition (N48h + Rhodiola algida var. tangutica group), showing shrinkage and chromatin compaction in normal MCF-7 breast cancer cells. d Morphology from MCF-7 breast cancer cells in adherent culture receiving Rhodiola algida var. tangutica (360 μg/mL) after exposure to hypoxia for 48 h (H48h + Rhodiola algida var. tangutica group).
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Fig2: Rhodiola algida var. tangutica inhibits hypoxia-induced proliferation in MCF-7 cells. The cells were plated at a seeding density of 5 × 104 viable cells/cm2 and grown as a monolayer in a 37°C incubator with a humidified atmosphere of 5% CO2 in a 2.0% O2 or normoxic. The images were obtained using 20× objectives 48 h after plating. a Morphology from normal MCF-7 breast cancer cells in adherent culture exposed to normoxic 48 h (N48h). b Morphology from MCF-7 breast cancer cells in adherent culture exposed to hypoxia 48 h (H48h), showing proliferation after exposure to hypoxia. c Morphology from MCF-7 breast cancer cells in adherent culture receiving Rhodiola algida var. tangutica (360 μg/mL) for 48 h under normoxic condition (N48h + Rhodiola algida var. tangutica group), showing shrinkage and chromatin compaction in normal MCF-7 breast cancer cells. d Morphology from MCF-7 breast cancer cells in adherent culture receiving Rhodiola algida var. tangutica (360 μg/mL) after exposure to hypoxia for 48 h (H48h + Rhodiola algida var. tangutica group).

Mentions: Morphological changes in MCF-7 breast cancer cells treated with R. algida extract under 2.0% O2 or normoxic condition were observed microscopically (Fig. 2). The cells showed shrinkage, chromatin compaction, and cytoplasmic vacuolation. Treatment with R. algida extract increased the apoptotic cell numbers (Fig. 2). Cell morphology was markedly changed after 48 h incubation with 360 μg/mL R. algida extract.Fig. 2


Effects of the aqueous extract of a Tibetan herb, Rhodiola algida var. tangutica on proliferation and HIF-1α, HIF-2α expression in MCF-7 cells under hypoxic condition in vitro.

Qi YJ, Cui S, Lu DX, Yang YZ, Luo Y, Ma L, Ma Y, Wuren T, Chang R, Qi L, Ben BJ, Han J, Ge RL - Cancer Cell Int. (2015)

Rhodiola algida var. tangutica inhibits hypoxia-induced proliferation in MCF-7 cells. The cells were plated at a seeding density of 5 × 104 viable cells/cm2 and grown as a monolayer in a 37°C incubator with a humidified atmosphere of 5% CO2 in a 2.0% O2 or normoxic. The images were obtained using 20× objectives 48 h after plating. a Morphology from normal MCF-7 breast cancer cells in adherent culture exposed to normoxic 48 h (N48h). b Morphology from MCF-7 breast cancer cells in adherent culture exposed to hypoxia 48 h (H48h), showing proliferation after exposure to hypoxia. c Morphology from MCF-7 breast cancer cells in adherent culture receiving Rhodiola algida var. tangutica (360 μg/mL) for 48 h under normoxic condition (N48h + Rhodiola algida var. tangutica group), showing shrinkage and chromatin compaction in normal MCF-7 breast cancer cells. d Morphology from MCF-7 breast cancer cells in adherent culture receiving Rhodiola algida var. tangutica (360 μg/mL) after exposure to hypoxia for 48 h (H48h + Rhodiola algida var. tangutica group).
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4536750&req=5

Fig2: Rhodiola algida var. tangutica inhibits hypoxia-induced proliferation in MCF-7 cells. The cells were plated at a seeding density of 5 × 104 viable cells/cm2 and grown as a monolayer in a 37°C incubator with a humidified atmosphere of 5% CO2 in a 2.0% O2 or normoxic. The images were obtained using 20× objectives 48 h after plating. a Morphology from normal MCF-7 breast cancer cells in adherent culture exposed to normoxic 48 h (N48h). b Morphology from MCF-7 breast cancer cells in adherent culture exposed to hypoxia 48 h (H48h), showing proliferation after exposure to hypoxia. c Morphology from MCF-7 breast cancer cells in adherent culture receiving Rhodiola algida var. tangutica (360 μg/mL) for 48 h under normoxic condition (N48h + Rhodiola algida var. tangutica group), showing shrinkage and chromatin compaction in normal MCF-7 breast cancer cells. d Morphology from MCF-7 breast cancer cells in adherent culture receiving Rhodiola algida var. tangutica (360 μg/mL) after exposure to hypoxia for 48 h (H48h + Rhodiola algida var. tangutica group).
Mentions: Morphological changes in MCF-7 breast cancer cells treated with R. algida extract under 2.0% O2 or normoxic condition were observed microscopically (Fig. 2). The cells showed shrinkage, chromatin compaction, and cytoplasmic vacuolation. Treatment with R. algida extract increased the apoptotic cell numbers (Fig. 2). Cell morphology was markedly changed after 48 h incubation with 360 μg/mL R. algida extract.Fig. 2

Bottom Line: Rhodiola algida var. tangutica is a traditional Tibetan herb.Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness.The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction.

View Article: PubMed Central - PubMed

Affiliation: Qinghai Province people's Hospital, Xining, 810007 Qinghai Peoples' Republic of China ; Research Center for High Altitude Medicine in Qinghai University, 16 Kunlun Road, Xining, 810001 Qinghai Peoples' Republic of China.

ABSTRACT

Ethnopharmacological relevance: Rhodiola algida var. tangutica is a traditional Tibetan herb. Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness. This study aimed to investigate the effect of Rhodiola algida var. tangutica on hypoxic MCF-7 breast cancer cells and the underlying mechanisms.

Materials and methods: The antiproliferative effects of R. algida on MCF-7 breast cancer cells were compared in vitro under hypoxic and normal conditions by using MTT analysis. The influence of R. algida on cancer cell apoptosis was determined by flow cytometry. The expression levels of hypoxia-inducible factor (HIF)-1α and HIF-2α were evaluated by western blot analysis.

Results: R. algida inhibited the proliferation of MCF-7 breast cancer cells in a dose- and time-dependent manner. The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction. Pretreatment with R. algida significantly suppressed the hypoxia-induced proliferation and expression of HIF-1α and HIF-2α in MCF-7 breast cancer cells.

Conclusions: R. algida might exert an anti-carcinogenic effect on MCF-7 breast cancer cells by decreasing the protein levels of HIF-1α and HIF-2α, which are overexpressed under hypoxic conditions. This effect might be elicited by inhibiting the hypoxia-induced proliferation of MCF-7 breast cancer cells.

No MeSH data available.


Related in: MedlinePlus