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Effects of the aqueous extract of a Tibetan herb, Rhodiola algida var. tangutica on proliferation and HIF-1α, HIF-2α expression in MCF-7 cells under hypoxic condition in vitro.

Qi YJ, Cui S, Lu DX, Yang YZ, Luo Y, Ma L, Ma Y, Wuren T, Chang R, Qi L, Ben BJ, Han J, Ge RL - Cancer Cell Int. (2015)

Bottom Line: Rhodiola algida var. tangutica is a traditional Tibetan herb.Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness.The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction.

View Article: PubMed Central - PubMed

Affiliation: Qinghai Province people's Hospital, Xining, 810007 Qinghai Peoples' Republic of China ; Research Center for High Altitude Medicine in Qinghai University, 16 Kunlun Road, Xining, 810001 Qinghai Peoples' Republic of China.

ABSTRACT

Ethnopharmacological relevance: Rhodiola algida var. tangutica is a traditional Tibetan herb. Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness. This study aimed to investigate the effect of Rhodiola algida var. tangutica on hypoxic MCF-7 breast cancer cells and the underlying mechanisms.

Materials and methods: The antiproliferative effects of R. algida on MCF-7 breast cancer cells were compared in vitro under hypoxic and normal conditions by using MTT analysis. The influence of R. algida on cancer cell apoptosis was determined by flow cytometry. The expression levels of hypoxia-inducible factor (HIF)-1α and HIF-2α were evaluated by western blot analysis.

Results: R. algida inhibited the proliferation of MCF-7 breast cancer cells in a dose- and time-dependent manner. The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction. Pretreatment with R. algida significantly suppressed the hypoxia-induced proliferation and expression of HIF-1α and HIF-2α in MCF-7 breast cancer cells.

Conclusions: R. algida might exert an anti-carcinogenic effect on MCF-7 breast cancer cells by decreasing the protein levels of HIF-1α and HIF-2α, which are overexpressed under hypoxic conditions. This effect might be elicited by inhibiting the hypoxia-induced proliferation of MCF-7 breast cancer cells.

No MeSH data available.


Related in: MedlinePlus

Effect of Rhodiola algida var. tangutica on the viability of MCF-7 cell. MCF-7 cell were cultured in microwells and incubated with increasing concentrations of Rhodiola algida var. tangutica 0, 6, 12, 24, 48 and 72 h after incubation with different concentrations of Rhodiola algida var. tangutica, percentage viability of MCF-7 cell was determined by trypan blue exclusion. The highest concentration (450 μg/mL) of Rhodiola algida var. tangutica caused a significant decrease in cell viability compared with control.
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Fig1: Effect of Rhodiola algida var. tangutica on the viability of MCF-7 cell. MCF-7 cell were cultured in microwells and incubated with increasing concentrations of Rhodiola algida var. tangutica 0, 6, 12, 24, 48 and 72 h after incubation with different concentrations of Rhodiola algida var. tangutica, percentage viability of MCF-7 cell was determined by trypan blue exclusion. The highest concentration (450 μg/mL) of Rhodiola algida var. tangutica caused a significant decrease in cell viability compared with control.

Mentions: The cytotoxicity of R. algida extract on MCF-7 cells was evaluated by the trypan blue dye exclusion method, and the appropriate concentrations of the plant extract to be used was determined. The MCF-7 (2 × 105 cells/well) breast cancer cells were incubated for 24 h in 96-well plates with various concentrations of R. algida extract (0, 45, 90, 180, 225, 360, and 450 μg/mL). Dose-dependent cytotoxic effect of the plant extract against MCF-7 breast cancer cells is shown in Fig. 1. The concentration of 450 μg/mL significantly decreased the number of viable cells and produced microscopic changes in morphology compared with those of the controls. Hence, the doses of 360 μg/mL or less were selected for subsequent experiments since these doses had weak cytotoxic effects on MCF-7 cells. However, with increasing incubation times, even low doses of R. algida extract significantly decreased cell viability.Fig. 1


Effects of the aqueous extract of a Tibetan herb, Rhodiola algida var. tangutica on proliferation and HIF-1α, HIF-2α expression in MCF-7 cells under hypoxic condition in vitro.

Qi YJ, Cui S, Lu DX, Yang YZ, Luo Y, Ma L, Ma Y, Wuren T, Chang R, Qi L, Ben BJ, Han J, Ge RL - Cancer Cell Int. (2015)

Effect of Rhodiola algida var. tangutica on the viability of MCF-7 cell. MCF-7 cell were cultured in microwells and incubated with increasing concentrations of Rhodiola algida var. tangutica 0, 6, 12, 24, 48 and 72 h after incubation with different concentrations of Rhodiola algida var. tangutica, percentage viability of MCF-7 cell was determined by trypan blue exclusion. The highest concentration (450 μg/mL) of Rhodiola algida var. tangutica caused a significant decrease in cell viability compared with control.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536750&req=5

Fig1: Effect of Rhodiola algida var. tangutica on the viability of MCF-7 cell. MCF-7 cell were cultured in microwells and incubated with increasing concentrations of Rhodiola algida var. tangutica 0, 6, 12, 24, 48 and 72 h after incubation with different concentrations of Rhodiola algida var. tangutica, percentage viability of MCF-7 cell was determined by trypan blue exclusion. The highest concentration (450 μg/mL) of Rhodiola algida var. tangutica caused a significant decrease in cell viability compared with control.
Mentions: The cytotoxicity of R. algida extract on MCF-7 cells was evaluated by the trypan blue dye exclusion method, and the appropriate concentrations of the plant extract to be used was determined. The MCF-7 (2 × 105 cells/well) breast cancer cells were incubated for 24 h in 96-well plates with various concentrations of R. algida extract (0, 45, 90, 180, 225, 360, and 450 μg/mL). Dose-dependent cytotoxic effect of the plant extract against MCF-7 breast cancer cells is shown in Fig. 1. The concentration of 450 μg/mL significantly decreased the number of viable cells and produced microscopic changes in morphology compared with those of the controls. Hence, the doses of 360 μg/mL or less were selected for subsequent experiments since these doses had weak cytotoxic effects on MCF-7 cells. However, with increasing incubation times, even low doses of R. algida extract significantly decreased cell viability.Fig. 1

Bottom Line: Rhodiola algida var. tangutica is a traditional Tibetan herb.Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness.The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction.

View Article: PubMed Central - PubMed

Affiliation: Qinghai Province people's Hospital, Xining, 810007 Qinghai Peoples' Republic of China ; Research Center for High Altitude Medicine in Qinghai University, 16 Kunlun Road, Xining, 810001 Qinghai Peoples' Republic of China.

ABSTRACT

Ethnopharmacological relevance: Rhodiola algida var. tangutica is a traditional Tibetan herb. Its root and rhizome have been successfully used as an effective clinical remedy for the prevention and treatment of cancer and high-altitude sickness. This study aimed to investigate the effect of Rhodiola algida var. tangutica on hypoxic MCF-7 breast cancer cells and the underlying mechanisms.

Materials and methods: The antiproliferative effects of R. algida on MCF-7 breast cancer cells were compared in vitro under hypoxic and normal conditions by using MTT analysis. The influence of R. algida on cancer cell apoptosis was determined by flow cytometry. The expression levels of hypoxia-inducible factor (HIF)-1α and HIF-2α were evaluated by western blot analysis.

Results: R. algida inhibited the proliferation of MCF-7 breast cancer cells in a dose- and time-dependent manner. The results of flow cytometry indicated that the antiproliferative effect of R. algida was mediated by apoptosis induction. Pretreatment with R. algida significantly suppressed the hypoxia-induced proliferation and expression of HIF-1α and HIF-2α in MCF-7 breast cancer cells.

Conclusions: R. algida might exert an anti-carcinogenic effect on MCF-7 breast cancer cells by decreasing the protein levels of HIF-1α and HIF-2α, which are overexpressed under hypoxic conditions. This effect might be elicited by inhibiting the hypoxia-induced proliferation of MCF-7 breast cancer cells.

No MeSH data available.


Related in: MedlinePlus