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Identification and characterization of the chromosomal yefM-yoeB toxin-antitoxin system of Streptococcus suis.

Zheng C, Xu J, Ren S, Li J, Xia M, Chen H, Bei W - Sci Rep (2015)

Bottom Line: Overproduction of S. suis YoeB toxin inhibited the growth of E. coli, and the toxicity of S. suis YoeB could be alleviated by the antitoxin YefM from S. suis and Streptococcus pneumoniae, but not by E. coli YefM.In a murine infection model, deletion of the yefM-yoeB locus had no effect on the virulence of S. suis serotype 2.Collectively, our data suggested that the yefM-yoeB locus of S. suis is an active TA system without the involvement of virulence.

View Article: PubMed Central - PubMed

Affiliation: 1] State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China [2] Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

ABSTRACT
Toxin-antitoxin (TA) systems are widely prevalent in the genomes of bacteria and archaea. These modules have been identified in Escherichia coli and various other bacteria. However, their presence in the genome of Streptococcus suis, an important zoonotic pathogen, has received little attention. In this study, we describe the identification and characterization of a type II TA system, comprising the chromosomal yefM-yoeB locus of S. suis. The yefM-yoeB locus is present in the genome of most serotypes of S. suis. Overproduction of S. suis YoeB toxin inhibited the growth of E. coli, and the toxicity of S. suis YoeB could be alleviated by the antitoxin YefM from S. suis and Streptococcus pneumoniae, but not by E. coli YefM. More importantly, introduction of the S. suis yefM-yoeB system into E. coli could affect cell growth. In a murine infection model, deletion of the yefM-yoeB locus had no effect on the virulence of S. suis serotype 2. Collectively, our data suggested that the yefM-yoeB locus of S. suis is an active TA system without the involvement of virulence.

No MeSH data available.


Related in: MedlinePlus

Growth curves of the WT, ΔyefM-yoeB and CΔyefM-yoeB strains.The WT, ΔyefM-yoeB and CΔyefM-yoeB strains were grown in TSB with 10% newborn bovine serum at 37 °C under static conditions. Samples were taken every hour to measure the OD600. The data shown are averages for the results from three independent experiments.
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f7: Growth curves of the WT, ΔyefM-yoeB and CΔyefM-yoeB strains.The WT, ΔyefM-yoeB and CΔyefM-yoeB strains were grown in TSB with 10% newborn bovine serum at 37 °C under static conditions. Samples were taken every hour to measure the OD600. The data shown are averages for the results from three independent experiments.

Mentions: The effects of yefM-yoeB deletion on the basic microbiological properties of S. suis 2 were investigated in terms of morphology, haemolytic activity and in vitro growth. The cell morphologies of the ΔyefM-yoeB mutant, WT and CΔyefM-yoeB strains were examined under light microscope using Gram staining. However, no obvious differences were found (see Supplementary Fig. S3a online). When inoculated on sheep blood agar plates, the three strains showed similar haemolytic activity (see Supplementary Fig. S3b online). The growth kinetics of the mutant strain were compared with those of the WT and complementation strains by measuring the optical density at 600 nm (OD600) every hour. We found that the growth kinetics of ΔyefM-yoeB were essentially identical to those of the WT and CΔyefM-yoeB strains (Fig. 7), indicating that the yefM-yoeB locus of S. suis 2 plays no role in growth in vitro.


Identification and characterization of the chromosomal yefM-yoeB toxin-antitoxin system of Streptococcus suis.

Zheng C, Xu J, Ren S, Li J, Xia M, Chen H, Bei W - Sci Rep (2015)

Growth curves of the WT, ΔyefM-yoeB and CΔyefM-yoeB strains.The WT, ΔyefM-yoeB and CΔyefM-yoeB strains were grown in TSB with 10% newborn bovine serum at 37 °C under static conditions. Samples were taken every hour to measure the OD600. The data shown are averages for the results from three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4536659&req=5

f7: Growth curves of the WT, ΔyefM-yoeB and CΔyefM-yoeB strains.The WT, ΔyefM-yoeB and CΔyefM-yoeB strains were grown in TSB with 10% newborn bovine serum at 37 °C under static conditions. Samples were taken every hour to measure the OD600. The data shown are averages for the results from three independent experiments.
Mentions: The effects of yefM-yoeB deletion on the basic microbiological properties of S. suis 2 were investigated in terms of morphology, haemolytic activity and in vitro growth. The cell morphologies of the ΔyefM-yoeB mutant, WT and CΔyefM-yoeB strains were examined under light microscope using Gram staining. However, no obvious differences were found (see Supplementary Fig. S3a online). When inoculated on sheep blood agar plates, the three strains showed similar haemolytic activity (see Supplementary Fig. S3b online). The growth kinetics of the mutant strain were compared with those of the WT and complementation strains by measuring the optical density at 600 nm (OD600) every hour. We found that the growth kinetics of ΔyefM-yoeB were essentially identical to those of the WT and CΔyefM-yoeB strains (Fig. 7), indicating that the yefM-yoeB locus of S. suis 2 plays no role in growth in vitro.

Bottom Line: Overproduction of S. suis YoeB toxin inhibited the growth of E. coli, and the toxicity of S. suis YoeB could be alleviated by the antitoxin YefM from S. suis and Streptococcus pneumoniae, but not by E. coli YefM.In a murine infection model, deletion of the yefM-yoeB locus had no effect on the virulence of S. suis serotype 2.Collectively, our data suggested that the yefM-yoeB locus of S. suis is an active TA system without the involvement of virulence.

View Article: PubMed Central - PubMed

Affiliation: 1] State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China [2] Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

ABSTRACT
Toxin-antitoxin (TA) systems are widely prevalent in the genomes of bacteria and archaea. These modules have been identified in Escherichia coli and various other bacteria. However, their presence in the genome of Streptococcus suis, an important zoonotic pathogen, has received little attention. In this study, we describe the identification and characterization of a type II TA system, comprising the chromosomal yefM-yoeB locus of S. suis. The yefM-yoeB locus is present in the genome of most serotypes of S. suis. Overproduction of S. suis YoeB toxin inhibited the growth of E. coli, and the toxicity of S. suis YoeB could be alleviated by the antitoxin YefM from S. suis and Streptococcus pneumoniae, but not by E. coli YefM. More importantly, introduction of the S. suis yefM-yoeB system into E. coli could affect cell growth. In a murine infection model, deletion of the yefM-yoeB locus had no effect on the virulence of S. suis serotype 2. Collectively, our data suggested that the yefM-yoeB locus of S. suis is an active TA system without the involvement of virulence.

No MeSH data available.


Related in: MedlinePlus