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Cerebrospinal Fluid Biomarkers of Japanese Encephalitis.

Sengupta N, Mukherjee S, Tripathi P, Kumar R, Suryavanshi A, Basu A - F1000Res (2015)

Bottom Line: Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders.With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost.  The present study identifies potential biomarkers for JE using a proteomics based approach.Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker.

View Article: PubMed Central - PubMed

Affiliation: National Brain Research Centre, Manesar, Haryana, 122051, India.

ABSTRACT
Japanese encephalitis (JE) is the leading cause of viral encephalitis in Asia. Acute encephalitis syndrome (AES) is a group of central nervous system (CNS) disorders caused by a wide range of viruses, bacteria, fungi, chemicals and toxins. It is important to distinguish between various forms of infectious encephalitis with similar clinical manifestations in order to ensure specific and accurate diagnosis and development of subsequent therapeutic strategies. Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders. With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost.  The present study identifies potential biomarkers for JE using a proteomics based approach. The CSF proteomes from ten patients each with JE and Non-JE acute encephalitis were analyzed by 2D gel electrophoresis followed by mass spectrometry. Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker.

No MeSH data available.


Related in: MedlinePlus

The associations of Vitamin D binding protein (‘GC’ in the figure) were explored using the STRING v10 clustering tool.The colored nodes signify a direct interaction with DBP whereas the white nodes denote a distant interaction. 4 proteins, LRP2 (yellow node), Cubilin (CUBN, green node), alpha 1 actin (ACTA1, blue node) and legumain (LGMN, violet node) are directly interacting with DBP and LRP2 is being shown the highest interacting score (0.983).
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f3: The associations of Vitamin D binding protein (‘GC’ in the figure) were explored using the STRING v10 clustering tool.The colored nodes signify a direct interaction with DBP whereas the white nodes denote a distant interaction. 4 proteins, LRP2 (yellow node), Cubilin (CUBN, green node), alpha 1 actin (ACTA1, blue node) and legumain (LGMN, violet node) are directly interacting with DBP and LRP2 is being shown the highest interacting score (0.983).

Mentions: The STRING v10 clustering tool was used to explore currently known associations of DBP (GC inFigure 3) and out of all the predicted functional partners, low density lipoprotein related protein-2 (LRP2) which is also known as megalin was found to have the highest interaction score (Figure 3).


Cerebrospinal Fluid Biomarkers of Japanese Encephalitis.

Sengupta N, Mukherjee S, Tripathi P, Kumar R, Suryavanshi A, Basu A - F1000Res (2015)

The associations of Vitamin D binding protein (‘GC’ in the figure) were explored using the STRING v10 clustering tool.The colored nodes signify a direct interaction with DBP whereas the white nodes denote a distant interaction. 4 proteins, LRP2 (yellow node), Cubilin (CUBN, green node), alpha 1 actin (ACTA1, blue node) and legumain (LGMN, violet node) are directly interacting with DBP and LRP2 is being shown the highest interacting score (0.983).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536617&req=5

f3: The associations of Vitamin D binding protein (‘GC’ in the figure) were explored using the STRING v10 clustering tool.The colored nodes signify a direct interaction with DBP whereas the white nodes denote a distant interaction. 4 proteins, LRP2 (yellow node), Cubilin (CUBN, green node), alpha 1 actin (ACTA1, blue node) and legumain (LGMN, violet node) are directly interacting with DBP and LRP2 is being shown the highest interacting score (0.983).
Mentions: The STRING v10 clustering tool was used to explore currently known associations of DBP (GC inFigure 3) and out of all the predicted functional partners, low density lipoprotein related protein-2 (LRP2) which is also known as megalin was found to have the highest interaction score (Figure 3).

Bottom Line: Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders.With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost.  The present study identifies potential biomarkers for JE using a proteomics based approach.Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker.

View Article: PubMed Central - PubMed

Affiliation: National Brain Research Centre, Manesar, Haryana, 122051, India.

ABSTRACT
Japanese encephalitis (JE) is the leading cause of viral encephalitis in Asia. Acute encephalitis syndrome (AES) is a group of central nervous system (CNS) disorders caused by a wide range of viruses, bacteria, fungi, chemicals and toxins. It is important to distinguish between various forms of infectious encephalitis with similar clinical manifestations in order to ensure specific and accurate diagnosis and development of subsequent therapeutic strategies. Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders. With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost.  The present study identifies potential biomarkers for JE using a proteomics based approach. The CSF proteomes from ten patients each with JE and Non-JE acute encephalitis were analyzed by 2D gel electrophoresis followed by mass spectrometry. Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker.

No MeSH data available.


Related in: MedlinePlus