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Cerebrospinal Fluid Biomarkers of Japanese Encephalitis.

Sengupta N, Mukherjee S, Tripathi P, Kumar R, Suryavanshi A, Basu A - F1000Res (2015)

Bottom Line: Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders.With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost.  The present study identifies potential biomarkers for JE using a proteomics based approach.Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker.

View Article: PubMed Central - PubMed

Affiliation: National Brain Research Centre, Manesar, Haryana, 122051, India.

ABSTRACT
Japanese encephalitis (JE) is the leading cause of viral encephalitis in Asia. Acute encephalitis syndrome (AES) is a group of central nervous system (CNS) disorders caused by a wide range of viruses, bacteria, fungi, chemicals and toxins. It is important to distinguish between various forms of infectious encephalitis with similar clinical manifestations in order to ensure specific and accurate diagnosis and development of subsequent therapeutic strategies. Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders. With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost.  The present study identifies potential biomarkers for JE using a proteomics based approach. The CSF proteomes from ten patients each with JE and Non-JE acute encephalitis were analyzed by 2D gel electrophoresis followed by mass spectrometry. Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker.

No MeSH data available.


Related in: MedlinePlus

(A and B). Inflammatory cytokine profile of cerebrospinal fluid from AES and JEV patients.IL-8, IL-1β, IL-6, IL-10, TNFα, and IL-12 concentrations were measured by flow cytometry using Human Inflammatory Cytokine Kit (BD Biosciences, San Diego, CA, USA) as per manufacturer’s instructions. Elevated levels of IL-1β and TNFα were observed in the JE samples (2A) whereas no significant changes were observed in the rest (2B). The image is a representative of 3 replicate experiments.
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f2: (A and B). Inflammatory cytokine profile of cerebrospinal fluid from AES and JEV patients.IL-8, IL-1β, IL-6, IL-10, TNFα, and IL-12 concentrations were measured by flow cytometry using Human Inflammatory Cytokine Kit (BD Biosciences, San Diego, CA, USA) as per manufacturer’s instructions. Elevated levels of IL-1β and TNFα were observed in the JE samples (2A) whereas no significant changes were observed in the rest (2B). The image is a representative of 3 replicate experiments.

Mentions: The levels of two pro-inflammatory cytokines IL-1β and TNFα were found to be significantly elevated in JE patients as compared to AES patients while there was no remarkable change in the rest (Figure 2). The data were analyzed by Student’s t-test and a statisticalp value<0.05 were considered significant.


Cerebrospinal Fluid Biomarkers of Japanese Encephalitis.

Sengupta N, Mukherjee S, Tripathi P, Kumar R, Suryavanshi A, Basu A - F1000Res (2015)

(A and B). Inflammatory cytokine profile of cerebrospinal fluid from AES and JEV patients.IL-8, IL-1β, IL-6, IL-10, TNFα, and IL-12 concentrations were measured by flow cytometry using Human Inflammatory Cytokine Kit (BD Biosciences, San Diego, CA, USA) as per manufacturer’s instructions. Elevated levels of IL-1β and TNFα were observed in the JE samples (2A) whereas no significant changes were observed in the rest (2B). The image is a representative of 3 replicate experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536617&req=5

f2: (A and B). Inflammatory cytokine profile of cerebrospinal fluid from AES and JEV patients.IL-8, IL-1β, IL-6, IL-10, TNFα, and IL-12 concentrations were measured by flow cytometry using Human Inflammatory Cytokine Kit (BD Biosciences, San Diego, CA, USA) as per manufacturer’s instructions. Elevated levels of IL-1β and TNFα were observed in the JE samples (2A) whereas no significant changes were observed in the rest (2B). The image is a representative of 3 replicate experiments.
Mentions: The levels of two pro-inflammatory cytokines IL-1β and TNFα were found to be significantly elevated in JE patients as compared to AES patients while there was no remarkable change in the rest (Figure 2). The data were analyzed by Student’s t-test and a statisticalp value<0.05 were considered significant.

Bottom Line: Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders.With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost.  The present study identifies potential biomarkers for JE using a proteomics based approach.Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker.

View Article: PubMed Central - PubMed

Affiliation: National Brain Research Centre, Manesar, Haryana, 122051, India.

ABSTRACT
Japanese encephalitis (JE) is the leading cause of viral encephalitis in Asia. Acute encephalitis syndrome (AES) is a group of central nervous system (CNS) disorders caused by a wide range of viruses, bacteria, fungi, chemicals and toxins. It is important to distinguish between various forms of infectious encephalitis with similar clinical manifestations in order to ensure specific and accurate diagnosis and development of subsequent therapeutic strategies. Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders. With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost.  The present study identifies potential biomarkers for JE using a proteomics based approach. The CSF proteomes from ten patients each with JE and Non-JE acute encephalitis were analyzed by 2D gel electrophoresis followed by mass spectrometry. Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker.

No MeSH data available.


Related in: MedlinePlus