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Cerebrospinal Fluid Biomarkers of Japanese Encephalitis.

Sengupta N, Mukherjee S, Tripathi P, Kumar R, Suryavanshi A, Basu A - F1000Res (2015)

Bottom Line: Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders.With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost.  The present study identifies potential biomarkers for JE using a proteomics based approach.Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker.

View Article: PubMed Central - PubMed

Affiliation: National Brain Research Centre, Manesar, Haryana, 122051, India.

ABSTRACT
Japanese encephalitis (JE) is the leading cause of viral encephalitis in Asia. Acute encephalitis syndrome (AES) is a group of central nervous system (CNS) disorders caused by a wide range of viruses, bacteria, fungi, chemicals and toxins. It is important to distinguish between various forms of infectious encephalitis with similar clinical manifestations in order to ensure specific and accurate diagnosis and development of subsequent therapeutic strategies. Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders. With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost.  The present study identifies potential biomarkers for JE using a proteomics based approach. The CSF proteomes from ten patients each with JE and Non-JE acute encephalitis were analyzed by 2D gel electrophoresis followed by mass spectrometry. Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker.

No MeSH data available.


Related in: MedlinePlus

Comparative proteomic analysis of cerebrospinal fluid from AES and JEV patients.Cerebrospinal fluid samples were pooled and proteins were extracted and separated on immobilized linear pH gradient IPG strips (pH 3.0–10.0) and then in the second dimension on 12% SDS-PAGE. Spots exclusively visualized in the JE- CSF were marked and excised, and identified by MALDITOF/MS and database searches. The spots are labeled on the gel according to the numbers presented inTable 2. Images are representative of 4 replicate experiments.
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f1: Comparative proteomic analysis of cerebrospinal fluid from AES and JEV patients.Cerebrospinal fluid samples were pooled and proteins were extracted and separated on immobilized linear pH gradient IPG strips (pH 3.0–10.0) and then in the second dimension on 12% SDS-PAGE. Spots exclusively visualized in the JE- CSF were marked and excised, and identified by MALDITOF/MS and database searches. The spots are labeled on the gel according to the numbers presented inTable 2. Images are representative of 4 replicate experiments.

Mentions: The specific JE associated proteins were identified by proteomic comparison of CSF from JEV-infected patients and patients with other forms of encephalitis. Around 16 proteins were found to be exclusively present in the JEV CSF proteome out of which 10 spots could be successfully identified (Figure 1). The observed MW and pI values of the protein spots on the 2- DE gels were compared with the theoretical MW and pI values of corresponding proteins (Table 2). The proteins identified were predominantly DBP, fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin. Most of the identified proteins were found to be members of the albumin multigene family.


Cerebrospinal Fluid Biomarkers of Japanese Encephalitis.

Sengupta N, Mukherjee S, Tripathi P, Kumar R, Suryavanshi A, Basu A - F1000Res (2015)

Comparative proteomic analysis of cerebrospinal fluid from AES and JEV patients.Cerebrospinal fluid samples were pooled and proteins were extracted and separated on immobilized linear pH gradient IPG strips (pH 3.0–10.0) and then in the second dimension on 12% SDS-PAGE. Spots exclusively visualized in the JE- CSF were marked and excised, and identified by MALDITOF/MS and database searches. The spots are labeled on the gel according to the numbers presented inTable 2. Images are representative of 4 replicate experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536617&req=5

f1: Comparative proteomic analysis of cerebrospinal fluid from AES and JEV patients.Cerebrospinal fluid samples were pooled and proteins were extracted and separated on immobilized linear pH gradient IPG strips (pH 3.0–10.0) and then in the second dimension on 12% SDS-PAGE. Spots exclusively visualized in the JE- CSF were marked and excised, and identified by MALDITOF/MS and database searches. The spots are labeled on the gel according to the numbers presented inTable 2. Images are representative of 4 replicate experiments.
Mentions: The specific JE associated proteins were identified by proteomic comparison of CSF from JEV-infected patients and patients with other forms of encephalitis. Around 16 proteins were found to be exclusively present in the JEV CSF proteome out of which 10 spots could be successfully identified (Figure 1). The observed MW and pI values of the protein spots on the 2- DE gels were compared with the theoretical MW and pI values of corresponding proteins (Table 2). The proteins identified were predominantly DBP, fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin. Most of the identified proteins were found to be members of the albumin multigene family.

Bottom Line: Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders.With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost.  The present study identifies potential biomarkers for JE using a proteomics based approach.Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker.

View Article: PubMed Central - PubMed

Affiliation: National Brain Research Centre, Manesar, Haryana, 122051, India.

ABSTRACT
Japanese encephalitis (JE) is the leading cause of viral encephalitis in Asia. Acute encephalitis syndrome (AES) is a group of central nervous system (CNS) disorders caused by a wide range of viruses, bacteria, fungi, chemicals and toxins. It is important to distinguish between various forms of infectious encephalitis with similar clinical manifestations in order to ensure specific and accurate diagnosis and development of subsequent therapeutic strategies. Cerebrospinal fluid (CSF) is in direct contact with the CNS and hence it is considered to be an excellent source for identifying biomarkers for various neurological disorders. With the recent advancement in proteomic methodologies, the field of biomarker research has received a remarkable boost.  The present study identifies potential biomarkers for JE using a proteomics based approach. The CSF proteomes from ten patients each with JE and Non-JE acute encephalitis were analyzed by 2D gel electrophoresis followed by mass spectrometry. Vitamin D-binding protein (DBP), fibrinogen gamma chain, fibrinogen beta chain, complement C4-B, complement C3 and cytoplasmic actin were found to be significantly elevated in case of JE indicating severe disruption of the blood brain barrier and DBP can be suggested to be an important diagnostic marker.

No MeSH data available.


Related in: MedlinePlus