Limits...
Characterization of STAT3 expression, signaling and inhibition in feline oral squamous cell carcinoma.

Brown ME, Bear MD, Rosol TJ, Premanandan C, Kisseberth WC, London CA - BMC Vet. Res. (2015)

Bottom Line: Exposure of SCCF2 cells to LLL12 resulted in decreased expression of pSTAT3 and total STAT3, apoptosis as assessed by caspase 3/7 activation, inhibition of colony formation and reduced expression of the STAT3 transcriptional target survivin.The OSCC cell lines with low basal STAT3 phosphorylation did not exhibit these effects, suggesting that STAT3 inhibition was responsible for the observed results.However, given the up-regulation of several STAT3 transcriptional targets following treatment, further investigation of STAT3 and its related signaling pathways in OSCC is warranted.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.

ABSTRACT

Background: Signal transducer and activator of transcription 3 (STAT3) plays a critical role in tumor development by regulating signaling pathways involved in cell proliferation, survival, metastasis and angiogenesis. STAT3 is activated in many cancers, including head and neck squamous cell carcinoma (HNSCC) in people. Feline oral squamous cell carcinoma (OSCC) is similar to advanced or recurrent HNSCC as it is poorly responsive to traditional therapies and carries a poor long-term prognosis. The purpose of this study was to characterize expression and activation of STAT3 in feline OSCC cell lines and tumor samples and to investigate the biologic activity of a novel, allosteric STAT3 inhibitor, LLL12, in feline OSCC cell lines.

Results: We evaluated 3 feline OSCC cell lines and one of these (SCCF2) exhibited high levels of constitutive STAT3 phosphorylation and high sensitivity to LLL12 treatment. Exposure of SCCF2 cells to LLL12 resulted in decreased expression of pSTAT3 and total STAT3, apoptosis as assessed by caspase 3/7 activation, inhibition of colony formation and reduced expression of the STAT3 transcriptional target survivin. In contrast, the STAT3 transcriptional targets VEGF and MCL-1 increased after LLL12 treatment. This was, in part, likely due to LLL12 mediated upregulation of HIF-1α, which is known to drive VEGF and MCL-1 expression. The OSCC cell lines with low basal STAT3 phosphorylation did not exhibit these effects, suggesting that STAT3 inhibition was responsible for the observed results. Lastly, immunohistochemistry for pSTAT3 was performed using a feline OSCC tissue microarray, demonstrating expression in 48 % of samples tested.

Conclusions: These data demonstrate that LLL12 has biologic activity against a feline OSCC cell line expressing pSTAT3 and that STAT3 represents a target for therapeutic intervention in this disease. However, given the up-regulation of several STAT3 transcriptional targets following treatment, further investigation of STAT3 and its related signaling pathways in OSCC is warranted.

No MeSH data available.


Related in: MedlinePlus

Activation of STAT3 in feline OSCC cell lines. Protein lysates were generated from the three untreated cell lines, separated by SDS-PAGE and Western blotting for pSTAT3 (Y705), STAT3 and β-actin was performed
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4536595&req=5

Fig1: Activation of STAT3 in feline OSCC cell lines. Protein lysates were generated from the three untreated cell lines, separated by SDS-PAGE and Western blotting for pSTAT3 (Y705), STAT3 and β-actin was performed

Mentions: To assess whether STAT3 is constitutively activated in feline OSCC cell lines, Western blotting was performed to evaluate for the presence of pSTAT3. As shown in Fig. 1, only one of the three cell lines, SCCF2, demonstrated high levels of pSTAT3 when compared to the others. All three OSCC cell lines were then incubated with increasing concentrations of LLL12 (0.01 μM – 10 μM) for 24 h and cell proliferation was assessed. While LLL12 significantly reduced cell proliferation in all cell lines (p < 0.005), SCCF2 was most sensitive to LLL12, with a calculated IC50 concentration in the nanomolar range (180 nM), correlating with the highest level of basal STAT3 phosphorylation (Fig. 2).Fig. 1


Characterization of STAT3 expression, signaling and inhibition in feline oral squamous cell carcinoma.

Brown ME, Bear MD, Rosol TJ, Premanandan C, Kisseberth WC, London CA - BMC Vet. Res. (2015)

Activation of STAT3 in feline OSCC cell lines. Protein lysates were generated from the three untreated cell lines, separated by SDS-PAGE and Western blotting for pSTAT3 (Y705), STAT3 and β-actin was performed
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536595&req=5

Fig1: Activation of STAT3 in feline OSCC cell lines. Protein lysates were generated from the three untreated cell lines, separated by SDS-PAGE and Western blotting for pSTAT3 (Y705), STAT3 and β-actin was performed
Mentions: To assess whether STAT3 is constitutively activated in feline OSCC cell lines, Western blotting was performed to evaluate for the presence of pSTAT3. As shown in Fig. 1, only one of the three cell lines, SCCF2, demonstrated high levels of pSTAT3 when compared to the others. All three OSCC cell lines were then incubated with increasing concentrations of LLL12 (0.01 μM – 10 μM) for 24 h and cell proliferation was assessed. While LLL12 significantly reduced cell proliferation in all cell lines (p < 0.005), SCCF2 was most sensitive to LLL12, with a calculated IC50 concentration in the nanomolar range (180 nM), correlating with the highest level of basal STAT3 phosphorylation (Fig. 2).Fig. 1

Bottom Line: Exposure of SCCF2 cells to LLL12 resulted in decreased expression of pSTAT3 and total STAT3, apoptosis as assessed by caspase 3/7 activation, inhibition of colony formation and reduced expression of the STAT3 transcriptional target survivin.The OSCC cell lines with low basal STAT3 phosphorylation did not exhibit these effects, suggesting that STAT3 inhibition was responsible for the observed results.However, given the up-regulation of several STAT3 transcriptional targets following treatment, further investigation of STAT3 and its related signaling pathways in OSCC is warranted.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.

ABSTRACT

Background: Signal transducer and activator of transcription 3 (STAT3) plays a critical role in tumor development by regulating signaling pathways involved in cell proliferation, survival, metastasis and angiogenesis. STAT3 is activated in many cancers, including head and neck squamous cell carcinoma (HNSCC) in people. Feline oral squamous cell carcinoma (OSCC) is similar to advanced or recurrent HNSCC as it is poorly responsive to traditional therapies and carries a poor long-term prognosis. The purpose of this study was to characterize expression and activation of STAT3 in feline OSCC cell lines and tumor samples and to investigate the biologic activity of a novel, allosteric STAT3 inhibitor, LLL12, in feline OSCC cell lines.

Results: We evaluated 3 feline OSCC cell lines and one of these (SCCF2) exhibited high levels of constitutive STAT3 phosphorylation and high sensitivity to LLL12 treatment. Exposure of SCCF2 cells to LLL12 resulted in decreased expression of pSTAT3 and total STAT3, apoptosis as assessed by caspase 3/7 activation, inhibition of colony formation and reduced expression of the STAT3 transcriptional target survivin. In contrast, the STAT3 transcriptional targets VEGF and MCL-1 increased after LLL12 treatment. This was, in part, likely due to LLL12 mediated upregulation of HIF-1α, which is known to drive VEGF and MCL-1 expression. The OSCC cell lines with low basal STAT3 phosphorylation did not exhibit these effects, suggesting that STAT3 inhibition was responsible for the observed results. Lastly, immunohistochemistry for pSTAT3 was performed using a feline OSCC tissue microarray, demonstrating expression in 48 % of samples tested.

Conclusions: These data demonstrate that LLL12 has biologic activity against a feline OSCC cell line expressing pSTAT3 and that STAT3 represents a target for therapeutic intervention in this disease. However, given the up-regulation of several STAT3 transcriptional targets following treatment, further investigation of STAT3 and its related signaling pathways in OSCC is warranted.

No MeSH data available.


Related in: MedlinePlus