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Binocular pattern deprivation interferes with the expression of proteins involved in primary visual cortex maturation in the cat.

Laskowska-Macios K, Nys J, Hu TT, Zapasnik M, Van der Perren A, Kossut M, Burnat K, Arckens L - Mol Brain (2015)

Bottom Line: Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region.Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 02-093, Warsaw, Poland. karolina.laskowska.macios@gmail.com.

ABSTRACT

Background: Binocular pattern deprivation from eye opening (early BD) delays the maturation of the primary visual cortex. This delay is more pronounced for the peripheral than the central visual field representation within area 17, particularly between the age of 2 and 4 months [Laskowska-Macios, Cereb Cortex, 2014].

Results: In this study, we probed for related dynamic changes in the cortical proteome. We introduced age, cortical region and BD as principal variables in a 2-D DIGE screen of area 17. In this way we explored the potential of BD-related protein expression changes between central and peripheral area 17 of 2- and 4-month-old BD (2BD, 4BD) kittens as a valid parameter towards the identification of brain maturation-related molecular processes. Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region. These BD-induced proteomic changes suggest a negative regulation of neurite outgrowth, synaptic transmission and clathrin-mediated endocytosis, thereby implicating these processes in normal experience-induced visual cortex maturation. Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].

Conclusions: Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.

No MeSH data available.


Related in: MedlinePlus

Semi-quantitative Western blotting for alpha-synuclein. Alpha-synuclein had a lower level in both area 17 regions of 2BD kittens and in the central region of the 4BD group as compared to age-matched normal controls. Additionally, a higher level of alpha-synuclein was observed in the central as compared to the peripheral region in 1N kittens. Asterisks above bars denote significant differences (P < 0.05) for a given region between age groups of a given condition (normal or BD). Numbers above BD-related bars denote the % statistical difference between BD and age-matched normal control groups (P < 0.05). Results are means with ± SD
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Fig5: Semi-quantitative Western blotting for alpha-synuclein. Alpha-synuclein had a lower level in both area 17 regions of 2BD kittens and in the central region of the 4BD group as compared to age-matched normal controls. Additionally, a higher level of alpha-synuclein was observed in the central as compared to the peripheral region in 1N kittens. Asterisks above bars denote significant differences (P < 0.05) for a given region between age groups of a given condition (normal or BD). Numbers above BD-related bars denote the % statistical difference between BD and age-matched normal control groups (P < 0.05). Results are means with ± SD

Mentions: Alpha-synuclein is involved in the regulation of vesicle storage and turnover as well as in the maturation and modulation of synaptic function [30–32]. It promotes SNARE-complex assembly during exocytosis in presynaptic terminals [32]. The Western blot experiment showed that specifically at the age of 1 month alpha-synuclein expression is lower in peripheral area 17. This centro-peripheral gradient is lost from 2N onwards. After the age of 6N, both cortical regions exhibit a drop to adult expression levels (Fig. 5). Western analysis confirmed the 2-D DIGE result showing a lower level of alpha-synuclein in the central region of 2BD kittens as compared to age-matched normal controls (Fig. 5). In 2N2BD subjects both central and peripheral area 17 showed a lower alpha-synuclein level as compared to age-matched normal controls, exhibiting a similar effect to that observed for early onset, age-matched 4BD kittens (Fig. 5).Fig. 5


Binocular pattern deprivation interferes with the expression of proteins involved in primary visual cortex maturation in the cat.

Laskowska-Macios K, Nys J, Hu TT, Zapasnik M, Van der Perren A, Kossut M, Burnat K, Arckens L - Mol Brain (2015)

Semi-quantitative Western blotting for alpha-synuclein. Alpha-synuclein had a lower level in both area 17 regions of 2BD kittens and in the central region of the 4BD group as compared to age-matched normal controls. Additionally, a higher level of alpha-synuclein was observed in the central as compared to the peripheral region in 1N kittens. Asterisks above bars denote significant differences (P < 0.05) for a given region between age groups of a given condition (normal or BD). Numbers above BD-related bars denote the % statistical difference between BD and age-matched normal control groups (P < 0.05). Results are means with ± SD
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536594&req=5

Fig5: Semi-quantitative Western blotting for alpha-synuclein. Alpha-synuclein had a lower level in both area 17 regions of 2BD kittens and in the central region of the 4BD group as compared to age-matched normal controls. Additionally, a higher level of alpha-synuclein was observed in the central as compared to the peripheral region in 1N kittens. Asterisks above bars denote significant differences (P < 0.05) for a given region between age groups of a given condition (normal or BD). Numbers above BD-related bars denote the % statistical difference between BD and age-matched normal control groups (P < 0.05). Results are means with ± SD
Mentions: Alpha-synuclein is involved in the regulation of vesicle storage and turnover as well as in the maturation and modulation of synaptic function [30–32]. It promotes SNARE-complex assembly during exocytosis in presynaptic terminals [32]. The Western blot experiment showed that specifically at the age of 1 month alpha-synuclein expression is lower in peripheral area 17. This centro-peripheral gradient is lost from 2N onwards. After the age of 6N, both cortical regions exhibit a drop to adult expression levels (Fig. 5). Western analysis confirmed the 2-D DIGE result showing a lower level of alpha-synuclein in the central region of 2BD kittens as compared to age-matched normal controls (Fig. 5). In 2N2BD subjects both central and peripheral area 17 showed a lower alpha-synuclein level as compared to age-matched normal controls, exhibiting a similar effect to that observed for early onset, age-matched 4BD kittens (Fig. 5).Fig. 5

Bottom Line: Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region.Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 02-093, Warsaw, Poland. karolina.laskowska.macios@gmail.com.

ABSTRACT

Background: Binocular pattern deprivation from eye opening (early BD) delays the maturation of the primary visual cortex. This delay is more pronounced for the peripheral than the central visual field representation within area 17, particularly between the age of 2 and 4 months [Laskowska-Macios, Cereb Cortex, 2014].

Results: In this study, we probed for related dynamic changes in the cortical proteome. We introduced age, cortical region and BD as principal variables in a 2-D DIGE screen of area 17. In this way we explored the potential of BD-related protein expression changes between central and peripheral area 17 of 2- and 4-month-old BD (2BD, 4BD) kittens as a valid parameter towards the identification of brain maturation-related molecular processes. Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region. These BD-induced proteomic changes suggest a negative regulation of neurite outgrowth, synaptic transmission and clathrin-mediated endocytosis, thereby implicating these processes in normal experience-induced visual cortex maturation. Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].

Conclusions: Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.

No MeSH data available.


Related in: MedlinePlus