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Binocular pattern deprivation interferes with the expression of proteins involved in primary visual cortex maturation in the cat.

Laskowska-Macios K, Nys J, Hu TT, Zapasnik M, Van der Perren A, Kossut M, Burnat K, Arckens L - Mol Brain (2015)

Bottom Line: Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region.Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 02-093, Warsaw, Poland. karolina.laskowska.macios@gmail.com.

ABSTRACT

Background: Binocular pattern deprivation from eye opening (early BD) delays the maturation of the primary visual cortex. This delay is more pronounced for the peripheral than the central visual field representation within area 17, particularly between the age of 2 and 4 months [Laskowska-Macios, Cereb Cortex, 2014].

Results: In this study, we probed for related dynamic changes in the cortical proteome. We introduced age, cortical region and BD as principal variables in a 2-D DIGE screen of area 17. In this way we explored the potential of BD-related protein expression changes between central and peripheral area 17 of 2- and 4-month-old BD (2BD, 4BD) kittens as a valid parameter towards the identification of brain maturation-related molecular processes. Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region. These BD-induced proteomic changes suggest a negative regulation of neurite outgrowth, synaptic transmission and clathrin-mediated endocytosis, thereby implicating these processes in normal experience-induced visual cortex maturation. Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].

Conclusions: Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.

No MeSH data available.


Related in: MedlinePlus

Proposed schematic overview of protein interactions. Interactions among detected proteins and proteins described in literature that are involved in processes with regard to the development of primary area 17: outgrowth, synaptic transmission, clathrin-mediated endocytosis (a) and metabolism (b). Interactions are accompanied by numbers, referring to corresponding references: 1. [85] 2. [86]; 3. [87]; 4. [88]; 5. [49]; 6. [89]; 7. [90]; 8. [91]; 9. [72]; 10. [92]; 11. [93]; 12. [94]; 13. [69]; 14. [95]; 15. [7]; 16. [96]; 17. [32]; 18. [97]; 19. [98]; 20. [99]; 21. [100]; 22. [101]; 23. [102]; 24. [103]; 25. [104]; 26. [105]; 27. [21]; 28. [25]. Grey rectangles – processes, rectangles with rounded corners – differential identified proteins; rectangles with rounded corners and dotted background – proteins studied by WB; proteins in an oval frame and dotted background – proteins studied by WB only; arrows – stimulation; line – interaction; perpendicular lines – inhibition
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Fig2: Proposed schematic overview of protein interactions. Interactions among detected proteins and proteins described in literature that are involved in processes with regard to the development of primary area 17: outgrowth, synaptic transmission, clathrin-mediated endocytosis (a) and metabolism (b). Interactions are accompanied by numbers, referring to corresponding references: 1. [85] 2. [86]; 3. [87]; 4. [88]; 5. [49]; 6. [89]; 7. [90]; 8. [91]; 9. [72]; 10. [92]; 11. [93]; 12. [94]; 13. [69]; 14. [95]; 15. [7]; 16. [96]; 17. [32]; 18. [97]; 19. [98]; 20. [99]; 21. [100]; 22. [101]; 23. [102]; 24. [103]; 25. [104]; 26. [105]; 27. [21]; 28. [25]. Grey rectangles – processes, rectangles with rounded corners – differential identified proteins; rectangles with rounded corners and dotted background – proteins studied by WB; proteins in an oval frame and dotted background – proteins studied by WB only; arrows – stimulation; line – interaction; perpendicular lines – inhibition

Mentions: Based on the IPA output we plotted a scheme presenting interactions between the identified proteins and their involvement in different biological processes. Figure 2a shows the interactions between 27 proteins related to three biological processes: outgrowth, clathrin-mediated endocytosis and synaptic transmission. Figure 2b illustrates a separate pathway linking the 11 proteins important for energy production. As eight out of the eleven identified mitochondrial energy metabolism-associated proteins had a higher expression in the 2BD group as compared to age-matched normal controls (Table 1), their upregulation suggests a higher energy demand to support neuronal activity and thus corresponds well with the previously revealed hyperactivity in area 17 of 2BD subjects [8].Fig. 2


Binocular pattern deprivation interferes with the expression of proteins involved in primary visual cortex maturation in the cat.

Laskowska-Macios K, Nys J, Hu TT, Zapasnik M, Van der Perren A, Kossut M, Burnat K, Arckens L - Mol Brain (2015)

Proposed schematic overview of protein interactions. Interactions among detected proteins and proteins described in literature that are involved in processes with regard to the development of primary area 17: outgrowth, synaptic transmission, clathrin-mediated endocytosis (a) and metabolism (b). Interactions are accompanied by numbers, referring to corresponding references: 1. [85] 2. [86]; 3. [87]; 4. [88]; 5. [49]; 6. [89]; 7. [90]; 8. [91]; 9. [72]; 10. [92]; 11. [93]; 12. [94]; 13. [69]; 14. [95]; 15. [7]; 16. [96]; 17. [32]; 18. [97]; 19. [98]; 20. [99]; 21. [100]; 22. [101]; 23. [102]; 24. [103]; 25. [104]; 26. [105]; 27. [21]; 28. [25]. Grey rectangles – processes, rectangles with rounded corners – differential identified proteins; rectangles with rounded corners and dotted background – proteins studied by WB; proteins in an oval frame and dotted background – proteins studied by WB only; arrows – stimulation; line – interaction; perpendicular lines – inhibition
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536594&req=5

Fig2: Proposed schematic overview of protein interactions. Interactions among detected proteins and proteins described in literature that are involved in processes with regard to the development of primary area 17: outgrowth, synaptic transmission, clathrin-mediated endocytosis (a) and metabolism (b). Interactions are accompanied by numbers, referring to corresponding references: 1. [85] 2. [86]; 3. [87]; 4. [88]; 5. [49]; 6. [89]; 7. [90]; 8. [91]; 9. [72]; 10. [92]; 11. [93]; 12. [94]; 13. [69]; 14. [95]; 15. [7]; 16. [96]; 17. [32]; 18. [97]; 19. [98]; 20. [99]; 21. [100]; 22. [101]; 23. [102]; 24. [103]; 25. [104]; 26. [105]; 27. [21]; 28. [25]. Grey rectangles – processes, rectangles with rounded corners – differential identified proteins; rectangles with rounded corners and dotted background – proteins studied by WB; proteins in an oval frame and dotted background – proteins studied by WB only; arrows – stimulation; line – interaction; perpendicular lines – inhibition
Mentions: Based on the IPA output we plotted a scheme presenting interactions between the identified proteins and their involvement in different biological processes. Figure 2a shows the interactions between 27 proteins related to three biological processes: outgrowth, clathrin-mediated endocytosis and synaptic transmission. Figure 2b illustrates a separate pathway linking the 11 proteins important for energy production. As eight out of the eleven identified mitochondrial energy metabolism-associated proteins had a higher expression in the 2BD group as compared to age-matched normal controls (Table 1), their upregulation suggests a higher energy demand to support neuronal activity and thus corresponds well with the previously revealed hyperactivity in area 17 of 2BD subjects [8].Fig. 2

Bottom Line: Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region.Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 02-093, Warsaw, Poland. karolina.laskowska.macios@gmail.com.

ABSTRACT

Background: Binocular pattern deprivation from eye opening (early BD) delays the maturation of the primary visual cortex. This delay is more pronounced for the peripheral than the central visual field representation within area 17, particularly between the age of 2 and 4 months [Laskowska-Macios, Cereb Cortex, 2014].

Results: In this study, we probed for related dynamic changes in the cortical proteome. We introduced age, cortical region and BD as principal variables in a 2-D DIGE screen of area 17. In this way we explored the potential of BD-related protein expression changes between central and peripheral area 17 of 2- and 4-month-old BD (2BD, 4BD) kittens as a valid parameter towards the identification of brain maturation-related molecular processes. Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region. These BD-induced proteomic changes suggest a negative regulation of neurite outgrowth, synaptic transmission and clathrin-mediated endocytosis, thereby implicating these processes in normal experience-induced visual cortex maturation. Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].

Conclusions: Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.

No MeSH data available.


Related in: MedlinePlus