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Binocular pattern deprivation interferes with the expression of proteins involved in primary visual cortex maturation in the cat.

Laskowska-Macios K, Nys J, Hu TT, Zapasnik M, Van der Perren A, Kossut M, Burnat K, Arckens L - Mol Brain (2015)

Bottom Line: Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region.Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 02-093, Warsaw, Poland. karolina.laskowska.macios@gmail.com.

ABSTRACT

Background: Binocular pattern deprivation from eye opening (early BD) delays the maturation of the primary visual cortex. This delay is more pronounced for the peripheral than the central visual field representation within area 17, particularly between the age of 2 and 4 months [Laskowska-Macios, Cereb Cortex, 2014].

Results: In this study, we probed for related dynamic changes in the cortical proteome. We introduced age, cortical region and BD as principal variables in a 2-D DIGE screen of area 17. In this way we explored the potential of BD-related protein expression changes between central and peripheral area 17 of 2- and 4-month-old BD (2BD, 4BD) kittens as a valid parameter towards the identification of brain maturation-related molecular processes. Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region. These BD-induced proteomic changes suggest a negative regulation of neurite outgrowth, synaptic transmission and clathrin-mediated endocytosis, thereby implicating these processes in normal experience-induced visual cortex maturation. Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].

Conclusions: Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.

No MeSH data available.


Related in: MedlinePlus

Tissue sampling for 2-D DIGE experiments. a Illustration of the tissue sampling in primary area 17: a frontal section of an in situ hybridization for zif268 in area 17 of a 2-month old normal kitten and the relevant level of the Rosenquist retinotopic map (1985). We have collected brain tissue from the central (C) and peripheral (P) visual field representation (White/black boxes) at Horsley-Clarke coordinate posterior 7 (P7.0). White/black lines demarcate the areal borders of area 17. Scale bar: 1 mm. b Visualization of all differential spots on an image of a preparative 2-D gel. Spot numbers match with the information in Table 1. Mw: molecular weight. c Venn diagrams illustrating the number of age-related differential spots as a function of cortical region (central or peripheral area 17) for normal and BD cats
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Fig1: Tissue sampling for 2-D DIGE experiments. a Illustration of the tissue sampling in primary area 17: a frontal section of an in situ hybridization for zif268 in area 17 of a 2-month old normal kitten and the relevant level of the Rosenquist retinotopic map (1985). We have collected brain tissue from the central (C) and peripheral (P) visual field representation (White/black boxes) at Horsley-Clarke coordinate posterior 7 (P7.0). White/black lines demarcate the areal borders of area 17. Scale bar: 1 mm. b Visualization of all differential spots on an image of a preparative 2-D gel. Spot numbers match with the information in Table 1. Mw: molecular weight. c Venn diagrams illustrating the number of age-related differential spots as a function of cortical region (central or peripheral area 17) for normal and BD cats

Mentions: Age, BD, and centro-peripheral expression differences were considered valid parameters to chart molecular events in relation to cortical maturation (Fig. 1a-c, Table 1).Fig. 1


Binocular pattern deprivation interferes with the expression of proteins involved in primary visual cortex maturation in the cat.

Laskowska-Macios K, Nys J, Hu TT, Zapasnik M, Van der Perren A, Kossut M, Burnat K, Arckens L - Mol Brain (2015)

Tissue sampling for 2-D DIGE experiments. a Illustration of the tissue sampling in primary area 17: a frontal section of an in situ hybridization for zif268 in area 17 of a 2-month old normal kitten and the relevant level of the Rosenquist retinotopic map (1985). We have collected brain tissue from the central (C) and peripheral (P) visual field representation (White/black boxes) at Horsley-Clarke coordinate posterior 7 (P7.0). White/black lines demarcate the areal borders of area 17. Scale bar: 1 mm. b Visualization of all differential spots on an image of a preparative 2-D gel. Spot numbers match with the information in Table 1. Mw: molecular weight. c Venn diagrams illustrating the number of age-related differential spots as a function of cortical region (central or peripheral area 17) for normal and BD cats
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4536594&req=5

Fig1: Tissue sampling for 2-D DIGE experiments. a Illustration of the tissue sampling in primary area 17: a frontal section of an in situ hybridization for zif268 in area 17 of a 2-month old normal kitten and the relevant level of the Rosenquist retinotopic map (1985). We have collected brain tissue from the central (C) and peripheral (P) visual field representation (White/black boxes) at Horsley-Clarke coordinate posterior 7 (P7.0). White/black lines demarcate the areal borders of area 17. Scale bar: 1 mm. b Visualization of all differential spots on an image of a preparative 2-D gel. Spot numbers match with the information in Table 1. Mw: molecular weight. c Venn diagrams illustrating the number of age-related differential spots as a function of cortical region (central or peripheral area 17) for normal and BD cats
Mentions: Age, BD, and centro-peripheral expression differences were considered valid parameters to chart molecular events in relation to cortical maturation (Fig. 1a-c, Table 1).Fig. 1

Bottom Line: Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region.Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neuroplasticity, Nencki Institute of Experimental Biology, 02-093, Warsaw, Poland. karolina.laskowska.macios@gmail.com.

ABSTRACT

Background: Binocular pattern deprivation from eye opening (early BD) delays the maturation of the primary visual cortex. This delay is more pronounced for the peripheral than the central visual field representation within area 17, particularly between the age of 2 and 4 months [Laskowska-Macios, Cereb Cortex, 2014].

Results: In this study, we probed for related dynamic changes in the cortical proteome. We introduced age, cortical region and BD as principal variables in a 2-D DIGE screen of area 17. In this way we explored the potential of BD-related protein expression changes between central and peripheral area 17 of 2- and 4-month-old BD (2BD, 4BD) kittens as a valid parameter towards the identification of brain maturation-related molecular processes. Consistent with the maturation delay, distinct developmental protein expression changes observed for normal kittens were postponed by BD, especially in the peripheral region. These BD-induced proteomic changes suggest a negative regulation of neurite outgrowth, synaptic transmission and clathrin-mediated endocytosis, thereby implicating these processes in normal experience-induced visual cortex maturation. Verification of the expression of proteins from each of the biological processes via Western analysis disclosed that some of the transient proteomic changes correlate to the distinct behavioral outcome in adult life, depending on timing and duration of the BD period [Neuroscience 2013;255:99-109].

Conclusions: Taken together, the plasticity potential to recover from BD, in relation to ensuing restoration of normal visual input, appears to rely on specific protein expression changes and cellular processes induced by the loss of pattern vision in early life.

No MeSH data available.


Related in: MedlinePlus