Limits...
Integrated analysis of miRNA/mRNA network in placenta identifies key factors associated with labor onset of Large White and Qingping sows.

Li H, Wu B, Geng J, Zhou J, Zheng R, Chai J, Li F, Peng J, Jiang S - Sci Rep (2015)

Bottom Line: A set of differentially expressed genes, including 2164 mRNAs and 39 miRNAs, were found.Cytoscape Network analysis of the functional genes found node mRNAs and that the regulatory network between the node mRNAs and miRNAs was established.A comparison of the sequencing data from the shorter gestation period (QS) and the normal gestation period (QL) indicated that these genes were responsible for the quicker and more sensitive reaction to the regulation of labour onset.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.

ABSTRACT
Labour onset is a very complex physiological process, and its mechanism is poorly understood. Here, we obtained the mRNA and miRNA expression profiles from the placentas of four groups of sows: Qingping sows 112 days after insemination with signs of labour onset (QS), Qingping sows 114 days after insemination with signs of labour onset (QL), Large White sows 114 days after insemination with signs of labour onset (LL) and Large White sows 112 days after insemination without signs of labour onset (LN). A set of differentially expressed genes, including 2164 mRNAs and 39 miRNAs, were found. A DAVID analysis of these differentially expressed genes revealed their critical roles in response to hormone stimulus, immune response. Cytoscape Network analysis of the functional genes found node mRNAs and that the regulatory network between the node mRNAs and miRNAs was established. A comparison of the sequencing data from the shorter gestation period (QS) and the normal gestation period (QL) indicated that these genes were responsible for the quicker and more sensitive reaction to the regulation of labour onset. This research not only detected the key factors that were involved in labour onset but also provided useful information for the research of gynaecological diseases.

No MeSH data available.


Related in: MedlinePlus

COX-2 and FOS expression in the amnion, chorion and decidua of LL and LN.(A) COX-2 mRNA level in the amnion, chorion and decidua of LL and LN as determined by qRT-PCR. (B) The expression levels of FOS in the amnion, chorion and decidua of LL and LN were detected by qRT-PCR. (C) COX-2 immunohistochemical results in the amnion, chorion and decidua of LL and LN. Original magnification, 400×. (D) FOS immunohistochemical result in the amnion, chorion and decidua of LL and LN. Original magnification, 400×. All of the error bars indicate SD. *P < 0.05, **P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4536519&req=5

f5: COX-2 and FOS expression in the amnion, chorion and decidua of LL and LN.(A) COX-2 mRNA level in the amnion, chorion and decidua of LL and LN as determined by qRT-PCR. (B) The expression levels of FOS in the amnion, chorion and decidua of LL and LN were detected by qRT-PCR. (C) COX-2 immunohistochemical results in the amnion, chorion and decidua of LL and LN. Original magnification, 400×. (D) FOS immunohistochemical result in the amnion, chorion and decidua of LL and LN. Original magnification, 400×. All of the error bars indicate SD. *P < 0.05, **P < 0.01.

Mentions: To study the DEmRNA functions, we performed a GO analysis of LL, QL, QS and overlapping DEGs. The top 10 GO terms are shown in Fig. 4. For DEGs, the GO term with the highest annotation value is involved in hormone stimulus, including “response to organic substance”, “response to endogenous stimulus”, “response to hormone stimulus”, “response to oestrogen stimulus” and “response to steroid hormone stimulus”, demonstrating the key roles of various hormones in the onset of labour, which is consistent with the previous theory (8,22). In addition, some representative functional terms are involved in immune function, such as “immune response”, “defence response”, and “inflammatory response”, and some enriched terms are connected with the triglyceride metabolic process, cytokine activity and regulation of cell growth. Cyclooxygenase 2 (COX2), also known as prostaglandin G/H synthase 2 (PGHS-2), is the rate-limiting enzyme in the biosynthesis of prostaglandins (PGs). According to the RNA-seq data, there is a 4-fold expression in LL compared with that in LN, and the high expression of COX2 leads to an increase in PGs. The relative quantitative expression of COX2 is significantly higher in LL than that in LN, even over 8-fold for amnion (Fig. 5A), which was confirmed by immunohistochemistry (Fig. 5C).


Integrated analysis of miRNA/mRNA network in placenta identifies key factors associated with labor onset of Large White and Qingping sows.

Li H, Wu B, Geng J, Zhou J, Zheng R, Chai J, Li F, Peng J, Jiang S - Sci Rep (2015)

COX-2 and FOS expression in the amnion, chorion and decidua of LL and LN.(A) COX-2 mRNA level in the amnion, chorion and decidua of LL and LN as determined by qRT-PCR. (B) The expression levels of FOS in the amnion, chorion and decidua of LL and LN were detected by qRT-PCR. (C) COX-2 immunohistochemical results in the amnion, chorion and decidua of LL and LN. Original magnification, 400×. (D) FOS immunohistochemical result in the amnion, chorion and decidua of LL and LN. Original magnification, 400×. All of the error bars indicate SD. *P < 0.05, **P < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4536519&req=5

f5: COX-2 and FOS expression in the amnion, chorion and decidua of LL and LN.(A) COX-2 mRNA level in the amnion, chorion and decidua of LL and LN as determined by qRT-PCR. (B) The expression levels of FOS in the amnion, chorion and decidua of LL and LN were detected by qRT-PCR. (C) COX-2 immunohistochemical results in the amnion, chorion and decidua of LL and LN. Original magnification, 400×. (D) FOS immunohistochemical result in the amnion, chorion and decidua of LL and LN. Original magnification, 400×. All of the error bars indicate SD. *P < 0.05, **P < 0.01.
Mentions: To study the DEmRNA functions, we performed a GO analysis of LL, QL, QS and overlapping DEGs. The top 10 GO terms are shown in Fig. 4. For DEGs, the GO term with the highest annotation value is involved in hormone stimulus, including “response to organic substance”, “response to endogenous stimulus”, “response to hormone stimulus”, “response to oestrogen stimulus” and “response to steroid hormone stimulus”, demonstrating the key roles of various hormones in the onset of labour, which is consistent with the previous theory (8,22). In addition, some representative functional terms are involved in immune function, such as “immune response”, “defence response”, and “inflammatory response”, and some enriched terms are connected with the triglyceride metabolic process, cytokine activity and regulation of cell growth. Cyclooxygenase 2 (COX2), also known as prostaglandin G/H synthase 2 (PGHS-2), is the rate-limiting enzyme in the biosynthesis of prostaglandins (PGs). According to the RNA-seq data, there is a 4-fold expression in LL compared with that in LN, and the high expression of COX2 leads to an increase in PGs. The relative quantitative expression of COX2 is significantly higher in LL than that in LN, even over 8-fold for amnion (Fig. 5A), which was confirmed by immunohistochemistry (Fig. 5C).

Bottom Line: A set of differentially expressed genes, including 2164 mRNAs and 39 miRNAs, were found.Cytoscape Network analysis of the functional genes found node mRNAs and that the regulatory network between the node mRNAs and miRNAs was established.A comparison of the sequencing data from the shorter gestation period (QS) and the normal gestation period (QL) indicated that these genes were responsible for the quicker and more sensitive reaction to the regulation of labour onset.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.

ABSTRACT
Labour onset is a very complex physiological process, and its mechanism is poorly understood. Here, we obtained the mRNA and miRNA expression profiles from the placentas of four groups of sows: Qingping sows 112 days after insemination with signs of labour onset (QS), Qingping sows 114 days after insemination with signs of labour onset (QL), Large White sows 114 days after insemination with signs of labour onset (LL) and Large White sows 112 days after insemination without signs of labour onset (LN). A set of differentially expressed genes, including 2164 mRNAs and 39 miRNAs, were found. A DAVID analysis of these differentially expressed genes revealed their critical roles in response to hormone stimulus, immune response. Cytoscape Network analysis of the functional genes found node mRNAs and that the regulatory network between the node mRNAs and miRNAs was established. A comparison of the sequencing data from the shorter gestation period (QS) and the normal gestation period (QL) indicated that these genes were responsible for the quicker and more sensitive reaction to the regulation of labour onset. This research not only detected the key factors that were involved in labour onset but also provided useful information for the research of gynaecological diseases.

No MeSH data available.


Related in: MedlinePlus