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Correlation between Polymorphism of TRAIL Gene and Condition of Intervertebral Disc Degeneration.

Xu S, Liang T, Li S - Med. Sci. Monit. (2015)

Bottom Line: At loci 1525 and 1529 in 3'-untranslated region (UTR) of 5th exon of TRAIL gene, 3 different genotypes were identified: experimental group had higher frequency of 1525CG/1595CC, 1525G and 1595C alleles, compared to the control group (p<0.05).Patients under Schneiderman grade IV had significantly higher allele frequency compared to those at grade II or III.Serum TRAIL level was also higher in the experimental group compared to the control group, and in grade IV patients compared to grade II or III patients (p<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Qingdao University, Qingdao, Shandong, China (mainland).

ABSTRACT

Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been suggested to be related with the pathogenesis and progression of osteoarticular degenerations. This study therefore aimed to investigate the relationship between the polymorphism of the TRAIL gene and the pathogenesis and severity of intervertebral disc degeneration (IDD) via detection of serum TRAIL expression levels.

Material and methods: A total of 100 IDD patients in our hospital were recruited in the experimental group, while another cohort of 100 healthy individuals was employed as the control group. Blood samples collected from all people were quantified for TRAIL level using enzyme-linked immunosorbent assay (ELISA), in addition to allele and genotype frequency analysis via fluorescent PCR for TRAIL gene.

Results: At loci 1525 and 1529 in 3'-untranslated region (UTR) of 5th exon of TRAIL gene, 3 different genotypes were identified: experimental group had higher frequency of 1525CG/1595CC, 1525G and 1595C alleles, compared to the control group (p<0.05). Patients under Schneiderman grade IV had significantly higher allele frequency compared to those at grade II or III. Serum TRAIL level was also higher in the experimental group compared to the control group, and in grade IV patients compared to grade II or III patients (p<0.05).

Conclusions: The G/C mutation at loci 1525/1595 of TRAIL gene may induce the progression of IDD, as the down-regulation of TRAIL can aggravate the severity of the disease.

No MeSH data available.


Related in: MedlinePlus

TRAIL gene polymorphism among IDD grades. (A) and (B) showed genotype frequency at loci 1525 and 1595, respectively. Higher grade patients had more 1525GG and 1595CC. (C) and (D) plotted allele frequency at those 2 loci. More 1525G and 1595C alleles occurred in higher grade IDD patients.
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f3-medscimonit-21-2282: TRAIL gene polymorphism among IDD grades. (A) and (B) showed genotype frequency at loci 1525 and 1595, respectively. Higher grade patients had more 1525GG and 1595CC. (C) and (D) plotted allele frequency at those 2 loci. More 1525G and 1595C alleles occurred in higher grade IDD patients.

Mentions: We further analyzed the gene polymorphism pattern of TRAIL gene among different clinical grades of IDD. Results (Figure 3) showed that patients with higher grade IDD had more genotypes including 1525GG (Figure 3A) and 1595CC (Figure 3B), along with alleles 1525G (Figure 3C) and 1595C (Figure 3D), although such differences were of insignificant differences under chi-square test.


Correlation between Polymorphism of TRAIL Gene and Condition of Intervertebral Disc Degeneration.

Xu S, Liang T, Li S - Med. Sci. Monit. (2015)

TRAIL gene polymorphism among IDD grades. (A) and (B) showed genotype frequency at loci 1525 and 1595, respectively. Higher grade patients had more 1525GG and 1595CC. (C) and (D) plotted allele frequency at those 2 loci. More 1525G and 1595C alleles occurred in higher grade IDD patients.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4532195&req=5

f3-medscimonit-21-2282: TRAIL gene polymorphism among IDD grades. (A) and (B) showed genotype frequency at loci 1525 and 1595, respectively. Higher grade patients had more 1525GG and 1595CC. (C) and (D) plotted allele frequency at those 2 loci. More 1525G and 1595C alleles occurred in higher grade IDD patients.
Mentions: We further analyzed the gene polymorphism pattern of TRAIL gene among different clinical grades of IDD. Results (Figure 3) showed that patients with higher grade IDD had more genotypes including 1525GG (Figure 3A) and 1595CC (Figure 3B), along with alleles 1525G (Figure 3C) and 1595C (Figure 3D), although such differences were of insignificant differences under chi-square test.

Bottom Line: At loci 1525 and 1529 in 3'-untranslated region (UTR) of 5th exon of TRAIL gene, 3 different genotypes were identified: experimental group had higher frequency of 1525CG/1595CC, 1525G and 1595C alleles, compared to the control group (p<0.05).Patients under Schneiderman grade IV had significantly higher allele frequency compared to those at grade II or III.Serum TRAIL level was also higher in the experimental group compared to the control group, and in grade IV patients compared to grade II or III patients (p<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Qingdao University, Qingdao, Shandong, China (mainland).

ABSTRACT

Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been suggested to be related with the pathogenesis and progression of osteoarticular degenerations. This study therefore aimed to investigate the relationship between the polymorphism of the TRAIL gene and the pathogenesis and severity of intervertebral disc degeneration (IDD) via detection of serum TRAIL expression levels.

Material and methods: A total of 100 IDD patients in our hospital were recruited in the experimental group, while another cohort of 100 healthy individuals was employed as the control group. Blood samples collected from all people were quantified for TRAIL level using enzyme-linked immunosorbent assay (ELISA), in addition to allele and genotype frequency analysis via fluorescent PCR for TRAIL gene.

Results: At loci 1525 and 1529 in 3'-untranslated region (UTR) of 5th exon of TRAIL gene, 3 different genotypes were identified: experimental group had higher frequency of 1525CG/1595CC, 1525G and 1595C alleles, compared to the control group (p<0.05). Patients under Schneiderman grade IV had significantly higher allele frequency compared to those at grade II or III. Serum TRAIL level was also higher in the experimental group compared to the control group, and in grade IV patients compared to grade II or III patients (p<0.05).

Conclusions: The G/C mutation at loci 1525/1595 of TRAIL gene may induce the progression of IDD, as the down-regulation of TRAIL can aggravate the severity of the disease.

No MeSH data available.


Related in: MedlinePlus