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Impaired polymorphonuclear leukocyte function in chronically hemodialyzed patients with iron overload.

Park W, Jang DH, Kim SH, Han CS, Shin WS, Kim HY, Kim DJ - Korean J. Intern. Med. (1988)

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ABSTRACT

Polymorphonuclear (PMN) leukoyte function tests and clinical data analyses were performed in 28 chronic renal failure (CRF) patients receiving regular hemodialysis. We divided them into two groups; 12 patients with normal serum ferritin were classified as group 1 and 16 patients with high serum ferritin as group 2. There was no difference in age, BUN, serum creatinine, complement (C3, C4), peripheral white blood cell count and the duration of dialysis between the two groups, but the serum iron level was higher in group 2 (129.1 ± 46.58 μg/dl) than in group 1 (74.3 ± 20.9 μg/dl) (p<0.001). The total iron binding capacity was lower in group 2 (p<0.05) and the number of transfusions was higher in group 2 (25 ± 16.1) than in group 1 (12 ± 8.7) (p<0.05). The nitroblue-tetrazolium (NBT) test showed no difference among groups 1, 2 and the healthy control group. In chemotaxis to fMLP (N-formylmethionylleucylphenylalanine), the mean number of migrated neutrophils to fMLP (10−6 M/L) per high power field was significantly decreased in group 2 (99.5 ± 37.6) compared with the healthy control group (140.1 ± 13.4) (p<0.005), but not in group 1 (155.8 ± 79.4). In the phagocytosis test using Staphylococcus aureus, the phagocytic index ratio compared to the healthy control group was significantly decreased in group 2 (0.59 ± 0.14), but not in group 1 (0.97 ± 0.18). These results suggest that iron overload due to multiple transfusions in patients receiving regular long term hemodialysis may play a part in causing susceptibility to infection by impairing PMN leukocyte functions, especially chemotaxis and phagocytosis.

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Mean percentage of NBT positive neutrophils.
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f2-kjim-3-1-64-10: Mean percentage of NBT positive neutrophils.

Mentions: In the NBT test, the mean number of neutrophils that reduced NBT dye out of 100 neutrophils is 4.6 ± 2.8 in seven healthy controls, 4.8 ± 3.3 in ten patients in group 1 and 4.5 ± 4.3 in eleven patients from group 2. There was no significant difference among the healthy controls and the two patient groups (Table 2) (Fig. 2).


Impaired polymorphonuclear leukocyte function in chronically hemodialyzed patients with iron overload.

Park W, Jang DH, Kim SH, Han CS, Shin WS, Kim HY, Kim DJ - Korean J. Intern. Med. (1988)

Mean percentage of NBT positive neutrophils.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4532132&req=5

f2-kjim-3-1-64-10: Mean percentage of NBT positive neutrophils.
Mentions: In the NBT test, the mean number of neutrophils that reduced NBT dye out of 100 neutrophils is 4.6 ± 2.8 in seven healthy controls, 4.8 ± 3.3 in ten patients in group 1 and 4.5 ± 4.3 in eleven patients from group 2. There was no significant difference among the healthy controls and the two patient groups (Table 2) (Fig. 2).

View Article: PubMed Central - PubMed

ABSTRACT

Polymorphonuclear (PMN) leukoyte function tests and clinical data analyses were performed in 28 chronic renal failure (CRF) patients receiving regular hemodialysis. We divided them into two groups; 12 patients with normal serum ferritin were classified as group 1 and 16 patients with high serum ferritin as group 2. There was no difference in age, BUN, serum creatinine, complement (C3, C4), peripheral white blood cell count and the duration of dialysis between the two groups, but the serum iron level was higher in group 2 (129.1 ± 46.58 μg/dl) than in group 1 (74.3 ± 20.9 μg/dl) (p<0.001). The total iron binding capacity was lower in group 2 (p<0.05) and the number of transfusions was higher in group 2 (25 ± 16.1) than in group 1 (12 ± 8.7) (p<0.05). The nitroblue-tetrazolium (NBT) test showed no difference among groups 1, 2 and the healthy control group. In chemotaxis to fMLP (N-formylmethionylleucylphenylalanine), the mean number of migrated neutrophils to fMLP (10−6 M/L) per high power field was significantly decreased in group 2 (99.5 ± 37.6) compared with the healthy control group (140.1 ± 13.4) (p<0.005), but not in group 1 (155.8 ± 79.4). In the phagocytosis test using Staphylococcus aureus, the phagocytic index ratio compared to the healthy control group was significantly decreased in group 2 (0.59 ± 0.14), but not in group 1 (0.97 ± 0.18). These results suggest that iron overload due to multiple transfusions in patients receiving regular long term hemodialysis may play a part in causing susceptibility to infection by impairing PMN leukocyte functions, especially chemotaxis and phagocytosis.

Show MeSH