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Effect of probenecid on tetraethyl ammonium (TEA) transport across basolateral membrane of rabbit proximal tubule.

Choi TL, Kim YK - Korean J. Intern. Med. (1992)

Bottom Line: Kinetic analysis indicated that probenecid decreased Vmax without a significant change in Km.However, probenecid did not significantly reduce TEA uptake in brush border and basolateral membrane vesicles prepared from renal cortex even at higher concentration of 10 mM.These results indicate that probenecid reduces TEA uptake in cortical slices by inhibiting the tissue metabolism rather than by the interaction with the organic cation transporter.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, St. Benedict Hospital, Pusan, Korea.

ABSTRACT
The effect of probenecid on the transport of tetraethylammonium (TEA) was investigated in rabbit reanal cortical slices in an attempt to ascertain the interaction of organic anion with the organic cation transport system in proximal tubule. Probenecid reversibly inhibited TEA uptake by cortical slices in a dose-dependent manner over the concentration range of 1 and 5 mM. The efflux of TEA was not affected by the presence of 3 mM probenecid. Kinetic analysis indicated that probenecid decreased Vmax without a significant change in Km. Probenecid inhibited significantly tissue oxygen consumption at concentrations of 3 and 5 mM. However, probenecid did not significantly reduce TEA uptake in brush border and basolateral membrane vesicles prepared from renal cortex even at higher concentration of 10 mM. These results indicate that probenecid reduces TEA uptake in cortical slices by inhibiting the tissue metabolism rather than by the interaction with the organic cation transporter.

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Lineweaver-Burk plot of the initial rate of active TEA accumulation into renal cortical slices in the presence and absence of 3 mM probenecid. TEA accumulation was determined during a 10-min incubation in a medium containing various concentrations of PAH and was corrected for passive accumulation which was determined in the presence of 10 mM choline chloride under 100% N2 atmosphere. Each point represents the mean ± SE of four experiments.
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f4-kjim-7-2-130-9: Lineweaver-Burk plot of the initial rate of active TEA accumulation into renal cortical slices in the presence and absence of 3 mM probenecid. TEA accumulation was determined during a 10-min incubation in a medium containing various concentrations of PAH and was corrected for passive accumulation which was determined in the presence of 10 mM choline chloride under 100% N2 atmosphere. Each point represents the mean ± SE of four experiments.

Mentions: Fig. 4. depicts Lineweaver-Burk plot of TEA uptake in the presence of 2 mM probenecid. Apparently probencid decreased the Vmax 1.87±0.29 to 1.0±0.14 μmole/g/10 min (Pä0.05) without a significant change in Km (0.33±0.07 vs. 0.29±0.08 mM).


Effect of probenecid on tetraethyl ammonium (TEA) transport across basolateral membrane of rabbit proximal tubule.

Choi TL, Kim YK - Korean J. Intern. Med. (1992)

Lineweaver-Burk plot of the initial rate of active TEA accumulation into renal cortical slices in the presence and absence of 3 mM probenecid. TEA accumulation was determined during a 10-min incubation in a medium containing various concentrations of PAH and was corrected for passive accumulation which was determined in the presence of 10 mM choline chloride under 100% N2 atmosphere. Each point represents the mean ± SE of four experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4532115&req=5

f4-kjim-7-2-130-9: Lineweaver-Burk plot of the initial rate of active TEA accumulation into renal cortical slices in the presence and absence of 3 mM probenecid. TEA accumulation was determined during a 10-min incubation in a medium containing various concentrations of PAH and was corrected for passive accumulation which was determined in the presence of 10 mM choline chloride under 100% N2 atmosphere. Each point represents the mean ± SE of four experiments.
Mentions: Fig. 4. depicts Lineweaver-Burk plot of TEA uptake in the presence of 2 mM probenecid. Apparently probencid decreased the Vmax 1.87±0.29 to 1.0±0.14 μmole/g/10 min (Pä0.05) without a significant change in Km (0.33±0.07 vs. 0.29±0.08 mM).

Bottom Line: Kinetic analysis indicated that probenecid decreased Vmax without a significant change in Km.However, probenecid did not significantly reduce TEA uptake in brush border and basolateral membrane vesicles prepared from renal cortex even at higher concentration of 10 mM.These results indicate that probenecid reduces TEA uptake in cortical slices by inhibiting the tissue metabolism rather than by the interaction with the organic cation transporter.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, St. Benedict Hospital, Pusan, Korea.

ABSTRACT
The effect of probenecid on the transport of tetraethylammonium (TEA) was investigated in rabbit reanal cortical slices in an attempt to ascertain the interaction of organic anion with the organic cation transport system in proximal tubule. Probenecid reversibly inhibited TEA uptake by cortical slices in a dose-dependent manner over the concentration range of 1 and 5 mM. The efflux of TEA was not affected by the presence of 3 mM probenecid. Kinetic analysis indicated that probenecid decreased Vmax without a significant change in Km. Probenecid inhibited significantly tissue oxygen consumption at concentrations of 3 and 5 mM. However, probenecid did not significantly reduce TEA uptake in brush border and basolateral membrane vesicles prepared from renal cortex even at higher concentration of 10 mM. These results indicate that probenecid reduces TEA uptake in cortical slices by inhibiting the tissue metabolism rather than by the interaction with the organic cation transporter.

Show MeSH
Related in: MedlinePlus