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Effect of probenecid on tetraethyl ammonium (TEA) transport across basolateral membrane of rabbit proximal tubule.

Choi TL, Kim YK - Korean J. Intern. Med. (1992)

Bottom Line: Kinetic analysis indicated that probenecid decreased Vmax without a significant change in Km.However, probenecid did not significantly reduce TEA uptake in brush border and basolateral membrane vesicles prepared from renal cortex even at higher concentration of 10 mM.These results indicate that probenecid reduces TEA uptake in cortical slices by inhibiting the tissue metabolism rather than by the interaction with the organic cation transporter.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, St. Benedict Hospital, Pusan, Korea.

ABSTRACT
The effect of probenecid on the transport of tetraethylammonium (TEA) was investigated in rabbit reanal cortical slices in an attempt to ascertain the interaction of organic anion with the organic cation transport system in proximal tubule. Probenecid reversibly inhibited TEA uptake by cortical slices in a dose-dependent manner over the concentration range of 1 and 5 mM. The efflux of TEA was not affected by the presence of 3 mM probenecid. Kinetic analysis indicated that probenecid decreased Vmax without a significant change in Km. Probenecid inhibited significantly tissue oxygen consumption at concentrations of 3 and 5 mM. However, probenecid did not significantly reduce TEA uptake in brush border and basolateral membrane vesicles prepared from renal cortex even at higher concentration of 10 mM. These results indicate that probenecid reduces TEA uptake in cortical slices by inhibiting the tissue metabolism rather than by the interaction with the organic cation transporter.

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Time course of TEA uptake by renal cortical slices in the hresence and absence of 3 mM probenecid. Each point represents the mean ± SE of three experiments.
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f1-kjim-7-2-130-9: Time course of TEA uptake by renal cortical slices in the hresence and absence of 3 mM probenecid. Each point represents the mean ± SE of three experiments.

Mentions: Fig. 1 shows the time course of TEA uptake by renal cortical slices in the presence and absence of 3 mM probenecid. Probenecid significantly inhibited TEA uptake. To examine the concentration dependence for the inhibitory effect of probenecid, TEA uptake was measured in the presence of various concentrations of probenecid. The results are shown in Fig. 2. Relative S/M ratios of TEA at the end of 60 min incubation were plotted against probenecid concentration in the medium. Probenecid significantly inhibited TEA uptake at higher concentrations than 1 mM.


Effect of probenecid on tetraethyl ammonium (TEA) transport across basolateral membrane of rabbit proximal tubule.

Choi TL, Kim YK - Korean J. Intern. Med. (1992)

Time course of TEA uptake by renal cortical slices in the hresence and absence of 3 mM probenecid. Each point represents the mean ± SE of three experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4532115&req=5

f1-kjim-7-2-130-9: Time course of TEA uptake by renal cortical slices in the hresence and absence of 3 mM probenecid. Each point represents the mean ± SE of three experiments.
Mentions: Fig. 1 shows the time course of TEA uptake by renal cortical slices in the presence and absence of 3 mM probenecid. Probenecid significantly inhibited TEA uptake. To examine the concentration dependence for the inhibitory effect of probenecid, TEA uptake was measured in the presence of various concentrations of probenecid. The results are shown in Fig. 2. Relative S/M ratios of TEA at the end of 60 min incubation were plotted against probenecid concentration in the medium. Probenecid significantly inhibited TEA uptake at higher concentrations than 1 mM.

Bottom Line: Kinetic analysis indicated that probenecid decreased Vmax without a significant change in Km.However, probenecid did not significantly reduce TEA uptake in brush border and basolateral membrane vesicles prepared from renal cortex even at higher concentration of 10 mM.These results indicate that probenecid reduces TEA uptake in cortical slices by inhibiting the tissue metabolism rather than by the interaction with the organic cation transporter.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, St. Benedict Hospital, Pusan, Korea.

ABSTRACT
The effect of probenecid on the transport of tetraethylammonium (TEA) was investigated in rabbit reanal cortical slices in an attempt to ascertain the interaction of organic anion with the organic cation transport system in proximal tubule. Probenecid reversibly inhibited TEA uptake by cortical slices in a dose-dependent manner over the concentration range of 1 and 5 mM. The efflux of TEA was not affected by the presence of 3 mM probenecid. Kinetic analysis indicated that probenecid decreased Vmax without a significant change in Km. Probenecid inhibited significantly tissue oxygen consumption at concentrations of 3 and 5 mM. However, probenecid did not significantly reduce TEA uptake in brush border and basolateral membrane vesicles prepared from renal cortex even at higher concentration of 10 mM. These results indicate that probenecid reduces TEA uptake in cortical slices by inhibiting the tissue metabolism rather than by the interaction with the organic cation transporter.

Show MeSH
Related in: MedlinePlus