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The effect of dilution, pH and ionic strength of plasma on t-PA precipitation in euglobulin fraction.

Hong SY, Shin HK, Yang DH - Korean J. Intern. Med. (1992)

Bottom Line: PAI Ag in euglobulin fraction showed consistent changes with t-PA Ag in the euglobulin fraction regardless of the changes in ionic strength and pH.Our results show that the currently used conditions for standard euglobulin precipitation are the most favorable for t-PA precipitation into the euglobulin fraction.The fibrinolytic activity exerted in the euglobulin fraction seems to depend on the amount of t-PA-PAI-1 complex rather than minimized protease inhibitor in the euglobulin fraction.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Soonchunhyang University College of Medicine, Chunan, Korea.

ABSTRACT
In order to evaluate the influence of dilution, pH and ionic strength on the precipitation of t-PA and PAI-1 during euglobulin precipitation, we measured t-PA Ag, PAI-1 Ag and fibrinolytic activity in the euglobulin fraction made of pooled plasma from liver cirrhosis patients, under various conditions by changing pH, ionic strength and degree of dilution. The precipitation of t-PA Ag in the euglobulin fraction was enhanced by decreasing the ionic strength and greatest at pH 6.0. The fibrinolytic activity in the euglobulin fraction showed consistent changes with t-PA Ag under varying pH and ionic strength. The precipitation of t-PA Ag was not influenced by the dilution factor but the larger the dilution factor, the greater the PAI-1 and the smaller the fibrinolytic activity in the euglobulin fraction. PAI Ag in euglobulin fraction showed consistent changes with t-PA Ag in the euglobulin fraction regardless of the changes in ionic strength and pH. The amount of precipitation of t-PA and PAI-1 was increased by the presence of dextran sulfate, under varying pH, ionic strength and dilution conditions. Our results show that the currently used conditions for standard euglobulin precipitation are the most favorable for t-PA precipitation into the euglobulin fraction. The fibrinolytic activity exerted in the euglobulin fraction seems to depend on the amount of t-PA-PAI-1 complex rather than minimized protease inhibitor in the euglobulin fraction.

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The effect of ionic strength on the euglobulin fibrinolytic activity, with or witout dextran sulfate. The euglobulin fraction prepared at a constant pH (5.8–6.0). Variation in ionic strength is recorded on the abscissa as final concentration of added NaCl in 1:10 diluted plasma. The fibrinolytic activity is higher in dextran sulfate euglobulin fraction regardless of the change in ionic strength.
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f7-kjim-7-2-73-1: The effect of ionic strength on the euglobulin fibrinolytic activity, with or witout dextran sulfate. The euglobulin fraction prepared at a constant pH (5.8–6.0). Variation in ionic strength is recorded on the abscissa as final concentration of added NaCl in 1:10 diluted plasma. The fibrinolytic activity is higher in dextran sulfate euglobulin fraction regardless of the change in ionic strength.

Mentions: The precipitation of PAI-1 Ag in the euglobulin fraction was enhanced by increasing the dilution factor (Fig. 6). Dextran sulfate increased the amount of precipitation by t-PA and PAI-1 under various pH, ionic strength and dilution (Fig. 7, 8, 9). More than 95% of t-PA (14/15 ng/ml) in plasma precipitated down in the euglobulin fraction, but PAI-1 was 45 ng/ml in plasma and about 8 ng/ml in euglobulin. This finding suggests that the standard method is more favorable to the precipitation of t-PA than PAI-1.


The effect of dilution, pH and ionic strength of plasma on t-PA precipitation in euglobulin fraction.

Hong SY, Shin HK, Yang DH - Korean J. Intern. Med. (1992)

The effect of ionic strength on the euglobulin fibrinolytic activity, with or witout dextran sulfate. The euglobulin fraction prepared at a constant pH (5.8–6.0). Variation in ionic strength is recorded on the abscissa as final concentration of added NaCl in 1:10 diluted plasma. The fibrinolytic activity is higher in dextran sulfate euglobulin fraction regardless of the change in ionic strength.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4532111&req=5

f7-kjim-7-2-73-1: The effect of ionic strength on the euglobulin fibrinolytic activity, with or witout dextran sulfate. The euglobulin fraction prepared at a constant pH (5.8–6.0). Variation in ionic strength is recorded on the abscissa as final concentration of added NaCl in 1:10 diluted plasma. The fibrinolytic activity is higher in dextran sulfate euglobulin fraction regardless of the change in ionic strength.
Mentions: The precipitation of PAI-1 Ag in the euglobulin fraction was enhanced by increasing the dilution factor (Fig. 6). Dextran sulfate increased the amount of precipitation by t-PA and PAI-1 under various pH, ionic strength and dilution (Fig. 7, 8, 9). More than 95% of t-PA (14/15 ng/ml) in plasma precipitated down in the euglobulin fraction, but PAI-1 was 45 ng/ml in plasma and about 8 ng/ml in euglobulin. This finding suggests that the standard method is more favorable to the precipitation of t-PA than PAI-1.

Bottom Line: PAI Ag in euglobulin fraction showed consistent changes with t-PA Ag in the euglobulin fraction regardless of the changes in ionic strength and pH.Our results show that the currently used conditions for standard euglobulin precipitation are the most favorable for t-PA precipitation into the euglobulin fraction.The fibrinolytic activity exerted in the euglobulin fraction seems to depend on the amount of t-PA-PAI-1 complex rather than minimized protease inhibitor in the euglobulin fraction.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Soonchunhyang University College of Medicine, Chunan, Korea.

ABSTRACT
In order to evaluate the influence of dilution, pH and ionic strength on the precipitation of t-PA and PAI-1 during euglobulin precipitation, we measured t-PA Ag, PAI-1 Ag and fibrinolytic activity in the euglobulin fraction made of pooled plasma from liver cirrhosis patients, under various conditions by changing pH, ionic strength and degree of dilution. The precipitation of t-PA Ag in the euglobulin fraction was enhanced by decreasing the ionic strength and greatest at pH 6.0. The fibrinolytic activity in the euglobulin fraction showed consistent changes with t-PA Ag under varying pH and ionic strength. The precipitation of t-PA Ag was not influenced by the dilution factor but the larger the dilution factor, the greater the PAI-1 and the smaller the fibrinolytic activity in the euglobulin fraction. PAI Ag in euglobulin fraction showed consistent changes with t-PA Ag in the euglobulin fraction regardless of the changes in ionic strength and pH. The amount of precipitation of t-PA and PAI-1 was increased by the presence of dextran sulfate, under varying pH, ionic strength and dilution conditions. Our results show that the currently used conditions for standard euglobulin precipitation are the most favorable for t-PA precipitation into the euglobulin fraction. The fibrinolytic activity exerted in the euglobulin fraction seems to depend on the amount of t-PA-PAI-1 complex rather than minimized protease inhibitor in the euglobulin fraction.

Show MeSH
Related in: MedlinePlus