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The expression of beta 1 integrin and intercellular adhesion molecule-1 on mesangial cells and its modulation in glomerulonephritis.

Ihm CG, Kim JP, Park JK, Kim BJ, Ahn JH, Lee TW, Yang MH, Kim MJ - Korean J. Intern. Med. (1994)

Bottom Line: The CM of MNC from Control suppressed the beta 1 integrin expression and thymidine incorporation and increased ICAM-1 expression on mesangial cells, compared to media alone.The expression of beta 1 integrin and ICAM-1, and the thymidine incorporation on mesangial cells are directly regulated by MNC, maybe through factors such as TGF beta or TNF alpha.In MsPGN, the overexpression of beta 1 integrin induced by MNC through TGF beta or hydroxyl radical on day 3 may be related to the pathogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Pathology, College of Medicine, KyungHee University, Seoul, Korea.

ABSTRACT

Objectives: This study was performed to observe the effects of conditioned media (CM) of cultured mononuclear cells (MNC) on the expression of beta 1 integrin and intercellular adhesion molecule(ICAM)-1 on mesangial cells and the modulation by TGF-beta, TNF alpha, or hydroxyl radical Also these were examined in anti-Thy mesangial proliferative GN (MsPGN) or puromycin aminonucleoside nephrosis(PAN).

Methods: The expression of beta 1 integrin and ICAM-1 on mesangial cells was examined by ELISA and the modulation by TGF-beta, TNF alpha, or hydroxyl radical was investigated by using neutralizing antibody and dimethylthiourea. [.OH] scavenger.

Results: The CM of MNC from Control suppressed the beta 1 integrin expression and thymidine incorporation and increased ICAM-1 expression on mesangial cells, compared to media alone. Especially the significant increase in ICAM-1 expression was reversed by anti-TNF alpha antibody. beta 1 integrin expression on mesangial cells was higher by the addition of CM of MNC in MsPGN than in Control, which was prevented by anti-TGF beta antibody or dimethylthiourea. ICAM-1 expression was not different among groups. Thymidine incorporation by adding CM of MNC was lower in MsPGN and PAN, which was mitigated by anti-TGF beta and anti-TNF alpha. Thus the ratio of the expression of ICAM-1 to thymidine incorporation was higher in MsPGN and PAN.

Conclusion: The expression of beta 1 integrin and ICAM-1, and the thymidine incorporation on mesangial cells are directly regulated by MNC, maybe through factors such as TGF beta or TNF alpha. In MsPGN, the overexpression of beta 1 integrin induced by MNC through TGF beta or hydroxyl radical on day 3 may be related to the pathogenesis.

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Related in: MedlinePlus

Effects of the conditioned media of mononuclear cells on the expression of β1 integrin and ICAM-1, and[3H] thymidine incorporation. The conditioned media of mononuclear cells from Control(■) suppressed the β1 integrin expression and thymidine incorporation, and increased ICAM-1 expression on mesangial cells, compared to media alone(□). a : p<0.05 vs. media alone.
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f1-kjim-9-2-93-7: Effects of the conditioned media of mononuclear cells on the expression of β1 integrin and ICAM-1, and[3H] thymidine incorporation. The conditioned media of mononuclear cells from Control(■) suppressed the β1 integrin expression and thymidine incorporation, and increased ICAM-1 expression on mesangial cells, compared to media alone(□). a : p<0.05 vs. media alone.

Mentions: The expression of β1 integrin was measured by ELISA on mesangial cells cultured for 48 h, after addition of CM of MNC harvested from rats with GN on day 3. The expression of β1 integrin on mesangial cells was decreased by 20%, compared to media alone, by the addition of CM of MNC from Control(Fig. 1). It was higher in MsPGN than in Control(n=6, 122 ± 12% vs. 102 ± 15%, p < 0.05)(Fig. 2). Further studies utilizing neutralizing antibody were perfomed to determine whether the above changes are modulated by TGF β. The increased expression was prevented by 25μg/ml anti-TGF β antibody(96 ± 11%)(Fig. 2). But, the treatment with anti-TGF β antibody also decreased the expression of β1 integrin in Control(71 ± 10%). Marginal increments in the expression of β1 integrin was noticed in PAN(113 ± 10%)(p<0.05).


The expression of beta 1 integrin and intercellular adhesion molecule-1 on mesangial cells and its modulation in glomerulonephritis.

Ihm CG, Kim JP, Park JK, Kim BJ, Ahn JH, Lee TW, Yang MH, Kim MJ - Korean J. Intern. Med. (1994)

Effects of the conditioned media of mononuclear cells on the expression of β1 integrin and ICAM-1, and[3H] thymidine incorporation. The conditioned media of mononuclear cells from Control(■) suppressed the β1 integrin expression and thymidine incorporation, and increased ICAM-1 expression on mesangial cells, compared to media alone(□). a : p<0.05 vs. media alone.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4532068&req=5

f1-kjim-9-2-93-7: Effects of the conditioned media of mononuclear cells on the expression of β1 integrin and ICAM-1, and[3H] thymidine incorporation. The conditioned media of mononuclear cells from Control(■) suppressed the β1 integrin expression and thymidine incorporation, and increased ICAM-1 expression on mesangial cells, compared to media alone(□). a : p<0.05 vs. media alone.
Mentions: The expression of β1 integrin was measured by ELISA on mesangial cells cultured for 48 h, after addition of CM of MNC harvested from rats with GN on day 3. The expression of β1 integrin on mesangial cells was decreased by 20%, compared to media alone, by the addition of CM of MNC from Control(Fig. 1). It was higher in MsPGN than in Control(n=6, 122 ± 12% vs. 102 ± 15%, p < 0.05)(Fig. 2). Further studies utilizing neutralizing antibody were perfomed to determine whether the above changes are modulated by TGF β. The increased expression was prevented by 25μg/ml anti-TGF β antibody(96 ± 11%)(Fig. 2). But, the treatment with anti-TGF β antibody also decreased the expression of β1 integrin in Control(71 ± 10%). Marginal increments in the expression of β1 integrin was noticed in PAN(113 ± 10%)(p<0.05).

Bottom Line: The CM of MNC from Control suppressed the beta 1 integrin expression and thymidine incorporation and increased ICAM-1 expression on mesangial cells, compared to media alone.The expression of beta 1 integrin and ICAM-1, and the thymidine incorporation on mesangial cells are directly regulated by MNC, maybe through factors such as TGF beta or TNF alpha.In MsPGN, the overexpression of beta 1 integrin induced by MNC through TGF beta or hydroxyl radical on day 3 may be related to the pathogenesis.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine and Pathology, College of Medicine, KyungHee University, Seoul, Korea.

ABSTRACT

Objectives: This study was performed to observe the effects of conditioned media (CM) of cultured mononuclear cells (MNC) on the expression of beta 1 integrin and intercellular adhesion molecule(ICAM)-1 on mesangial cells and the modulation by TGF-beta, TNF alpha, or hydroxyl radical Also these were examined in anti-Thy mesangial proliferative GN (MsPGN) or puromycin aminonucleoside nephrosis(PAN).

Methods: The expression of beta 1 integrin and ICAM-1 on mesangial cells was examined by ELISA and the modulation by TGF-beta, TNF alpha, or hydroxyl radical was investigated by using neutralizing antibody and dimethylthiourea. [.OH] scavenger.

Results: The CM of MNC from Control suppressed the beta 1 integrin expression and thymidine incorporation and increased ICAM-1 expression on mesangial cells, compared to media alone. Especially the significant increase in ICAM-1 expression was reversed by anti-TNF alpha antibody. beta 1 integrin expression on mesangial cells was higher by the addition of CM of MNC in MsPGN than in Control, which was prevented by anti-TGF beta antibody or dimethylthiourea. ICAM-1 expression was not different among groups. Thymidine incorporation by adding CM of MNC was lower in MsPGN and PAN, which was mitigated by anti-TGF beta and anti-TNF alpha. Thus the ratio of the expression of ICAM-1 to thymidine incorporation was higher in MsPGN and PAN.

Conclusion: The expression of beta 1 integrin and ICAM-1, and the thymidine incorporation on mesangial cells are directly regulated by MNC, maybe through factors such as TGF beta or TNF alpha. In MsPGN, the overexpression of beta 1 integrin induced by MNC through TGF beta or hydroxyl radical on day 3 may be related to the pathogenesis.

Show MeSH
Related in: MedlinePlus