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Tumor necrosis factor alpha from peripheral blood mononuclear cells of IgA nephropathy and mesangial cell proliferation.

Lee TW, Ahn JH, Park JK, Ihm CG, Kim MJ - Korean J. Intern. Med. (1994)

Bottom Line: In ten patients with primary IgA nephropathy and 10 normal controls, PBMC were cultured with PHA (20 micrograms/ml) and con-A (10 micrograms/ml) for 24 hours, and supernatants were collected and stored at -70 degrees C until tested.The cytokine concentrations of PBMC culture supernatant were measured by radioimmunoassay.It is suggested that the mononuclear cell of IgA nephropathy may have the intrinsic property to produce more TNF-alpha which may be mitogenic to mesangial cells when stimulated with mitogens, and it may be related to the mesangial cell proliferation in IgA nephropathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine/Nephrology, Kyung-Hee University College of Medicine, Seoul, Korea.

ABSTRACT

Background: To investigate the role of mononuclear cells and their products on rat mesangial cell proliferation, we evaluated the effect of peripheral blood mononuclear cells (PBMC) culture supernatant of patients with IgA nephropathy on rat mesangial cells 3[H]-thymidine uptake, and measured the concentration of cytokines in the supernatant to find out which cytokine in PBMC culture supernatant has a major influence on mesangial cell 3[H]-thymidine uptake.

Methods: In ten patients with primary IgA nephropathy and 10 normal controls, PBMC were cultured with PHA (20 micrograms/ml) and con-A (10 micrograms/ml) for 24 hours, and supernatants were collected and stored at -70 degrees C until tested. Mesangial cell was cultured for 48 hours at a concentration of 10(4) cells/well with 1:2 or 1:10 diluted PBMC culture supernatant. 3[H]-thymidine uptake into rat mesangial cell was assayed after 18 hour culture. The cytokine concentrations of PBMC culture supernatant were measured by radioimmunoassay.

Results: When 1:2 diluted PBMC culture supernatant was added, the supernatant of IgA nephropathy induced higher 3[H]-thymidine uptake into mesangial cell compared with that of normal controls. 3[H]-thymidine uptake induced by 1:2 diluted PBMC culture supernatant was significantly higher than that induced by 1:10 diluted supernatant of IgA nephropathy. The concentration of TNF-alpha in PBMC culture supernatant of the patients with IgA nephropathy was higher than that of normal controls and showed a good correlation with the mesangial cell 3[H]-thymidine uptake.

Conclusion: It is suggested that the mononuclear cell of IgA nephropathy may have the intrinsic property to produce more TNF-alpha which may be mitogenic to mesangial cells when stimulated with mitogens, and it may be related to the mesangial cell proliferation in IgA nephropathy.

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Correlation between concentrations of TNF α in PBMC culture supernatant and mesangial cell 3[H]-thymidine uptake.
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f3-kjim-9-1-1-1: Correlation between concentrations of TNF α in PBMC culture supernatant and mesangial cell 3[H]-thymidine uptake.

Mentions: The concentration of TNF α in PBMC culture supernatant of patients with IgA nephropathy was significantly higher than that of normal controls (136.1±35.0 fmol/L vs 28.2±19.0 fmol/L, p<0.05). Moreover, the concentration of TNF α was significantly correlated with the mesangial cell 3[H]-thymidine uptake in patients with IgA nephropathy (r=0.61, p = 0.002) (Fig. 3). The concentration of IL -1β, IL-2, IFN-γ, and PDGF was higher in PBMC culture supernatant of patients with IgA nephropathy compared with that of normal controls. However, no statistical significance was observed (Fig. 2).


Tumor necrosis factor alpha from peripheral blood mononuclear cells of IgA nephropathy and mesangial cell proliferation.

Lee TW, Ahn JH, Park JK, Ihm CG, Kim MJ - Korean J. Intern. Med. (1994)

Correlation between concentrations of TNF α in PBMC culture supernatant and mesangial cell 3[H]-thymidine uptake.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4532055&req=5

f3-kjim-9-1-1-1: Correlation between concentrations of TNF α in PBMC culture supernatant and mesangial cell 3[H]-thymidine uptake.
Mentions: The concentration of TNF α in PBMC culture supernatant of patients with IgA nephropathy was significantly higher than that of normal controls (136.1±35.0 fmol/L vs 28.2±19.0 fmol/L, p<0.05). Moreover, the concentration of TNF α was significantly correlated with the mesangial cell 3[H]-thymidine uptake in patients with IgA nephropathy (r=0.61, p = 0.002) (Fig. 3). The concentration of IL -1β, IL-2, IFN-γ, and PDGF was higher in PBMC culture supernatant of patients with IgA nephropathy compared with that of normal controls. However, no statistical significance was observed (Fig. 2).

Bottom Line: In ten patients with primary IgA nephropathy and 10 normal controls, PBMC were cultured with PHA (20 micrograms/ml) and con-A (10 micrograms/ml) for 24 hours, and supernatants were collected and stored at -70 degrees C until tested.The cytokine concentrations of PBMC culture supernatant were measured by radioimmunoassay.It is suggested that the mononuclear cell of IgA nephropathy may have the intrinsic property to produce more TNF-alpha which may be mitogenic to mesangial cells when stimulated with mitogens, and it may be related to the mesangial cell proliferation in IgA nephropathy.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine/Nephrology, Kyung-Hee University College of Medicine, Seoul, Korea.

ABSTRACT

Background: To investigate the role of mononuclear cells and their products on rat mesangial cell proliferation, we evaluated the effect of peripheral blood mononuclear cells (PBMC) culture supernatant of patients with IgA nephropathy on rat mesangial cells 3[H]-thymidine uptake, and measured the concentration of cytokines in the supernatant to find out which cytokine in PBMC culture supernatant has a major influence on mesangial cell 3[H]-thymidine uptake.

Methods: In ten patients with primary IgA nephropathy and 10 normal controls, PBMC were cultured with PHA (20 micrograms/ml) and con-A (10 micrograms/ml) for 24 hours, and supernatants were collected and stored at -70 degrees C until tested. Mesangial cell was cultured for 48 hours at a concentration of 10(4) cells/well with 1:2 or 1:10 diluted PBMC culture supernatant. 3[H]-thymidine uptake into rat mesangial cell was assayed after 18 hour culture. The cytokine concentrations of PBMC culture supernatant were measured by radioimmunoassay.

Results: When 1:2 diluted PBMC culture supernatant was added, the supernatant of IgA nephropathy induced higher 3[H]-thymidine uptake into mesangial cell compared with that of normal controls. 3[H]-thymidine uptake induced by 1:2 diluted PBMC culture supernatant was significantly higher than that induced by 1:10 diluted supernatant of IgA nephropathy. The concentration of TNF-alpha in PBMC culture supernatant of the patients with IgA nephropathy was higher than that of normal controls and showed a good correlation with the mesangial cell 3[H]-thymidine uptake.

Conclusion: It is suggested that the mononuclear cell of IgA nephropathy may have the intrinsic property to produce more TNF-alpha which may be mitogenic to mesangial cells when stimulated with mitogens, and it may be related to the mesangial cell proliferation in IgA nephropathy.

Show MeSH
Related in: MedlinePlus