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Establishment of BALB/c mice model infected with Helicobacter pylori.

Jin DZ, Jung HC, Kim JM, Kim JS, Song IS, Kim CY - Korean J. Intern. Med. (1999)

Bottom Line: Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized.Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group.Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Korea.

ABSTRACT

Objectives: Considering the geographic differences in the prevalence of virulence factors such as CagA or VacA of H. pylori isolated from Korean adults compared with those from western countries, the establishment of a mouse model infected with H. pylori isolated from Korean adults is needed to investigate the pathogenesis and to develop vaccines against H. pylori infection in Korea. The aim of this study was to establish the BALB/c mouse model infected with H. pylori isolated from Korean.

Methods: Six-week-old BALB/c mice were inoculated intragastrically with 10(9) CFU of H. pylori. Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized. Evidence for H. pylori infection was assessed by rapid urease test of gastric mucosa and by microscopic examination using the H & E stain and Warthin-Starry silver stain.

Results: Rapid urease test was positive in 55% of all inoculated mice. Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group. Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation.

Conclusions: The H. pylori isolated freshly from Korean adults could colonize the stomach of BALB/c mice and induce pathologic alterations that mimics human gastric diseases. This model would facilitate the investigations for the pathogenetic mechanisms of H. pylori infection.

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Related in: MedlinePlus

ELISA for serum IgG response to H. pylori sonicate antigen. Serum IgG reaction to H. pylori sonicate antigen became positive at 1 week post-inoculation and began to increase significantly at 2 weeks post-inoculation compared with that of control mice. Each panel represents the mean±SE of 3 separate experiments.
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f9-kjim-14-2-55-9: ELISA for serum IgG response to H. pylori sonicate antigen. Serum IgG reaction to H. pylori sonicate antigen became positive at 1 week post-inoculation and began to increase significantly at 2 weeks post-inoculation compared with that of control mice. Each panel represents the mean±SE of 3 separate experiments.

Mentions: The experimentally infected mice showed serum antibody response to the colonizing strain that could be detected by an ELISA of mice sera from the first week of infection. Anti-H. pylori IgG antibody levels increased markedly from the second week after the last inoculation with H. pylori and reached the plateau after the fourth week. In contrast, the negligible serum antibody response was shown in the control group during the entire experiment period (Fig. 9).


Establishment of BALB/c mice model infected with Helicobacter pylori.

Jin DZ, Jung HC, Kim JM, Kim JS, Song IS, Kim CY - Korean J. Intern. Med. (1999)

ELISA for serum IgG response to H. pylori sonicate antigen. Serum IgG reaction to H. pylori sonicate antigen became positive at 1 week post-inoculation and began to increase significantly at 2 weeks post-inoculation compared with that of control mice. Each panel represents the mean±SE of 3 separate experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4531914&req=5

f9-kjim-14-2-55-9: ELISA for serum IgG response to H. pylori sonicate antigen. Serum IgG reaction to H. pylori sonicate antigen became positive at 1 week post-inoculation and began to increase significantly at 2 weeks post-inoculation compared with that of control mice. Each panel represents the mean±SE of 3 separate experiments.
Mentions: The experimentally infected mice showed serum antibody response to the colonizing strain that could be detected by an ELISA of mice sera from the first week of infection. Anti-H. pylori IgG antibody levels increased markedly from the second week after the last inoculation with H. pylori and reached the plateau after the fourth week. In contrast, the negligible serum antibody response was shown in the control group during the entire experiment period (Fig. 9).

Bottom Line: Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized.Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group.Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Korea.

ABSTRACT

Objectives: Considering the geographic differences in the prevalence of virulence factors such as CagA or VacA of H. pylori isolated from Korean adults compared with those from western countries, the establishment of a mouse model infected with H. pylori isolated from Korean adults is needed to investigate the pathogenesis and to develop vaccines against H. pylori infection in Korea. The aim of this study was to establish the BALB/c mouse model infected with H. pylori isolated from Korean.

Methods: Six-week-old BALB/c mice were inoculated intragastrically with 10(9) CFU of H. pylori. Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized. Evidence for H. pylori infection was assessed by rapid urease test of gastric mucosa and by microscopic examination using the H & E stain and Warthin-Starry silver stain.

Results: Rapid urease test was positive in 55% of all inoculated mice. Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group. Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation.

Conclusions: The H. pylori isolated freshly from Korean adults could colonize the stomach of BALB/c mice and induce pathologic alterations that mimics human gastric diseases. This model would facilitate the investigations for the pathogenetic mechanisms of H. pylori infection.

Show MeSH
Related in: MedlinePlus