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Establishment of BALB/c mice model infected with Helicobacter pylori.

Jin DZ, Jung HC, Kim JM, Kim JS, Song IS, Kim CY - Korean J. Intern. Med. (1999)

Bottom Line: Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized.Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group.Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Korea.

ABSTRACT

Objectives: Considering the geographic differences in the prevalence of virulence factors such as CagA or VacA of H. pylori isolated from Korean adults compared with those from western countries, the establishment of a mouse model infected with H. pylori isolated from Korean adults is needed to investigate the pathogenesis and to develop vaccines against H. pylori infection in Korea. The aim of this study was to establish the BALB/c mouse model infected with H. pylori isolated from Korean.

Methods: Six-week-old BALB/c mice were inoculated intragastrically with 10(9) CFU of H. pylori. Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized. Evidence for H. pylori infection was assessed by rapid urease test of gastric mucosa and by microscopic examination using the H & E stain and Warthin-Starry silver stain.

Results: Rapid urease test was positive in 55% of all inoculated mice. Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group. Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation.

Conclusions: The H. pylori isolated freshly from Korean adults could colonize the stomach of BALB/c mice and induce pathologic alterations that mimics human gastric diseases. This model would facilitate the investigations for the pathogenetic mechanisms of H. pylori infection.

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Related in: MedlinePlus

Characterization of virulence factors of H. pylori by PCR. Each H. pylori strain isolated from human (before inoculation to mouse; lane 2, 3, 4, 5) and mouse (lane 6, 7, 8, 9) show identical pattern of virulence factors such as vacA1, vacA2, vac3 and cagA. M; molecular marker (φX174/HaeIII).
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f7-kjim-14-2-55-9: Characterization of virulence factors of H. pylori by PCR. Each H. pylori strain isolated from human (before inoculation to mouse; lane 2, 3, 4, 5) and mouse (lane 6, 7, 8, 9) show identical pattern of virulence factors such as vacA1, vacA2, vac3 and cagA. M; molecular marker (φX174/HaeIII).

Mentions: Isolates from infected mice showed typical morphology and biochemical characteristics of H. pylori as the same as the original H. pylori strain (data not shown). Furthermore, the Helicobacter-specific PCR showed that the mouse and human isolates were identical to H. pylori (Fig. 6). The original human clinical isolates and the mouse isolates were also positive for cagA and vacA by PCR (Fig. 7). A comparison of the genomic DNA by RAPD PCR was performed for the mouse isolates and four original human clinical isolates which were used to infect the mouse. The mouse isolates gave an identical band pattern to only one (strain # 7) of the four human isolates (Fig. 8). These results suggest that strain # 7 may be the one that is able to colonize the mouse gastric mucosa among all the human isolates tested.


Establishment of BALB/c mice model infected with Helicobacter pylori.

Jin DZ, Jung HC, Kim JM, Kim JS, Song IS, Kim CY - Korean J. Intern. Med. (1999)

Characterization of virulence factors of H. pylori by PCR. Each H. pylori strain isolated from human (before inoculation to mouse; lane 2, 3, 4, 5) and mouse (lane 6, 7, 8, 9) show identical pattern of virulence factors such as vacA1, vacA2, vac3 and cagA. M; molecular marker (φX174/HaeIII).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4531914&req=5

f7-kjim-14-2-55-9: Characterization of virulence factors of H. pylori by PCR. Each H. pylori strain isolated from human (before inoculation to mouse; lane 2, 3, 4, 5) and mouse (lane 6, 7, 8, 9) show identical pattern of virulence factors such as vacA1, vacA2, vac3 and cagA. M; molecular marker (φX174/HaeIII).
Mentions: Isolates from infected mice showed typical morphology and biochemical characteristics of H. pylori as the same as the original H. pylori strain (data not shown). Furthermore, the Helicobacter-specific PCR showed that the mouse and human isolates were identical to H. pylori (Fig. 6). The original human clinical isolates and the mouse isolates were also positive for cagA and vacA by PCR (Fig. 7). A comparison of the genomic DNA by RAPD PCR was performed for the mouse isolates and four original human clinical isolates which were used to infect the mouse. The mouse isolates gave an identical band pattern to only one (strain # 7) of the four human isolates (Fig. 8). These results suggest that strain # 7 may be the one that is able to colonize the mouse gastric mucosa among all the human isolates tested.

Bottom Line: Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized.Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group.Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Korea.

ABSTRACT

Objectives: Considering the geographic differences in the prevalence of virulence factors such as CagA or VacA of H. pylori isolated from Korean adults compared with those from western countries, the establishment of a mouse model infected with H. pylori isolated from Korean adults is needed to investigate the pathogenesis and to develop vaccines against H. pylori infection in Korea. The aim of this study was to establish the BALB/c mouse model infected with H. pylori isolated from Korean.

Methods: Six-week-old BALB/c mice were inoculated intragastrically with 10(9) CFU of H. pylori. Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized. Evidence for H. pylori infection was assessed by rapid urease test of gastric mucosa and by microscopic examination using the H & E stain and Warthin-Starry silver stain.

Results: Rapid urease test was positive in 55% of all inoculated mice. Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group. Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation.

Conclusions: The H. pylori isolated freshly from Korean adults could colonize the stomach of BALB/c mice and induce pathologic alterations that mimics human gastric diseases. This model would facilitate the investigations for the pathogenetic mechanisms of H. pylori infection.

Show MeSH
Related in: MedlinePlus