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Establishment of BALB/c mice model infected with Helicobacter pylori.

Jin DZ, Jung HC, Kim JM, Kim JS, Song IS, Kim CY - Korean J. Intern. Med. (1999)

Bottom Line: Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized.Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group.Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Korea.

ABSTRACT

Objectives: Considering the geographic differences in the prevalence of virulence factors such as CagA or VacA of H. pylori isolated from Korean adults compared with those from western countries, the establishment of a mouse model infected with H. pylori isolated from Korean adults is needed to investigate the pathogenesis and to develop vaccines against H. pylori infection in Korea. The aim of this study was to establish the BALB/c mouse model infected with H. pylori isolated from Korean.

Methods: Six-week-old BALB/c mice were inoculated intragastrically with 10(9) CFU of H. pylori. Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized. Evidence for H. pylori infection was assessed by rapid urease test of gastric mucosa and by microscopic examination using the H & E stain and Warthin-Starry silver stain.

Results: Rapid urease test was positive in 55% of all inoculated mice. Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group. Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation.

Conclusions: The H. pylori isolated freshly from Korean adults could colonize the stomach of BALB/c mice and induce pathologic alterations that mimics human gastric diseases. This model would facilitate the investigations for the pathogenetic mechanisms of H. pylori infection.

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Related in: MedlinePlus

Gastric mucosa of BALB/c mouse infected with H. pylori for 6 weeks. An extensive infiltration of inflammatory cells (arrow) is seen. H & E stain. Original magnification, × 200.
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f2-kjim-14-2-55-9: Gastric mucosa of BALB/c mouse infected with H. pylori for 6 weeks. An extensive infiltration of inflammatory cells (arrow) is seen. H & E stain. Original magnification, × 200.

Mentions: In the first week after the last inoculation, no definite presence of bacteria and infiltration of inflammatory cells were shown in H. pylori-infected group (Fig. 1). However, the inflammatory infiltrates of lymphocytes, plasma cells and polymorphonuclear leukocytes in the antrum and corpus mucosa were gradually increased from 2 weeks after the last inoculation of H. pylori. At six weeks after the last inoculation, the gastric lesions were characterized by definite inflammatory infiltrates accompanied by the disruption of gastric architecture (Fig. 2, 3) and distinct H. pylori colonization (Fig. 4). Control mice showed no evidence of significant infiltration of inflammatory cells during the entire experimental period (Fig. 5).


Establishment of BALB/c mice model infected with Helicobacter pylori.

Jin DZ, Jung HC, Kim JM, Kim JS, Song IS, Kim CY - Korean J. Intern. Med. (1999)

Gastric mucosa of BALB/c mouse infected with H. pylori for 6 weeks. An extensive infiltration of inflammatory cells (arrow) is seen. H & E stain. Original magnification, × 200.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4531914&req=5

f2-kjim-14-2-55-9: Gastric mucosa of BALB/c mouse infected with H. pylori for 6 weeks. An extensive infiltration of inflammatory cells (arrow) is seen. H & E stain. Original magnification, × 200.
Mentions: In the first week after the last inoculation, no definite presence of bacteria and infiltration of inflammatory cells were shown in H. pylori-infected group (Fig. 1). However, the inflammatory infiltrates of lymphocytes, plasma cells and polymorphonuclear leukocytes in the antrum and corpus mucosa were gradually increased from 2 weeks after the last inoculation of H. pylori. At six weeks after the last inoculation, the gastric lesions were characterized by definite inflammatory infiltrates accompanied by the disruption of gastric architecture (Fig. 2, 3) and distinct H. pylori colonization (Fig. 4). Control mice showed no evidence of significant infiltration of inflammatory cells during the entire experimental period (Fig. 5).

Bottom Line: Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized.Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group.Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Korea.

ABSTRACT

Objectives: Considering the geographic differences in the prevalence of virulence factors such as CagA or VacA of H. pylori isolated from Korean adults compared with those from western countries, the establishment of a mouse model infected with H. pylori isolated from Korean adults is needed to investigate the pathogenesis and to develop vaccines against H. pylori infection in Korea. The aim of this study was to establish the BALB/c mouse model infected with H. pylori isolated from Korean.

Methods: Six-week-old BALB/c mice were inoculated intragastrically with 10(9) CFU of H. pylori. Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized. Evidence for H. pylori infection was assessed by rapid urease test of gastric mucosa and by microscopic examination using the H & E stain and Warthin-Starry silver stain.

Results: Rapid urease test was positive in 55% of all inoculated mice. Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group. Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation.

Conclusions: The H. pylori isolated freshly from Korean adults could colonize the stomach of BALB/c mice and induce pathologic alterations that mimics human gastric diseases. This model would facilitate the investigations for the pathogenetic mechanisms of H. pylori infection.

Show MeSH
Related in: MedlinePlus