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The Gut Microbiota of Wild Mice.

Weldon L, Abolins S, Lenzi L, Bourne C, Riley EM, Viney M - PLoS ONE (2015)

Bottom Line: The gut microbiota profoundly affects the biology of its host.The composition of the microbiota is dynamic and is affected by both host genetic and many environmental effects.In this study we have characterised the gut (both the caecum and rectum) microbiota of wild caught Mus musculus domesticus at three UK sites and have investigated how the microbiota varies depending on host location and host characteristics.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, University of Bristol, Bristol, United Kingdom.

ABSTRACT
The gut microbiota profoundly affects the biology of its host. The composition of the microbiota is dynamic and is affected by both host genetic and many environmental effects. The gut microbiota of laboratory mice has been studied extensively, which has uncovered many of the effects that the microbiota can have. This work has also shown that the environments of different research institutions can affect the mouse microbiota. There has been relatively limited study of the microbiota of wild mice, but this has shown that it typically differs from that of laboratory mice (and that maintaining wild caught mice in the laboratory can quite quickly alter the microbiota). There is also inter-individual variation in the microbiota of wild mice, with this principally explained by geographical location. In this study we have characterised the gut (both the caecum and rectum) microbiota of wild caught Mus musculus domesticus at three UK sites and have investigated how the microbiota varies depending on host location and host characteristics. We find that the microbiota of these mice are generally consistent with those described from other wild mice. The rectal and caecal microbiotas of individual mice are generally more similar to each other, than they are to the microbiota of other individuals. We found significant differences in the diversity of the microbiotas among mice from different sample sites. There were significant correlations of microbiota diversity and body weight, a measure of age, body-mass index, serum concentration of leptin, and virus, nematode and mite infection.

No MeSH data available.


Related in: MedlinePlus

Bacterial Families.A histogram showing the relative proportion of different bacterial families present in 39 samples from 14 mice, where the two letter prefix denotes the location from where the mouse was captured and the three digit number is the unique mouse identifier; C and R are caecal and rectal samples, respectively, all as Table 1. For a bacterial family to be shown its abundance was ≥3% in at least one sample; bacterial families whose abundance was below this criterion are grouped in the category ‘rare’.
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pone.0134643.g002: Bacterial Families.A histogram showing the relative proportion of different bacterial families present in 39 samples from 14 mice, where the two letter prefix denotes the location from where the mouse was captured and the three digit number is the unique mouse identifier; C and R are caecal and rectal samples, respectively, all as Table 1. For a bacterial family to be shown its abundance was ≥3% in at least one sample; bacterial families whose abundance was below this criterion are grouped in the category ‘rare’.

Mentions: At the bacterial family level, Lachnospiraceae and Ruminococcaceae (both Firmicutes) were the most common (mean abundance of 47% (SD 15%) and 15% (SD 11%), respectively) (Fig 2). Taxa of these families are associated with the maintenance of gut health and, while they contain functionally diverse taxa, they share a common role as active degraders of plant-derived material in the gut [56]. Our identification of OTUs by sequence homology to family level was very high, consistent with previous work showing that unknown or poorly described microbes in wild house mice are comparatively rare [35].


The Gut Microbiota of Wild Mice.

Weldon L, Abolins S, Lenzi L, Bourne C, Riley EM, Viney M - PLoS ONE (2015)

Bacterial Families.A histogram showing the relative proportion of different bacterial families present in 39 samples from 14 mice, where the two letter prefix denotes the location from where the mouse was captured and the three digit number is the unique mouse identifier; C and R are caecal and rectal samples, respectively, all as Table 1. For a bacterial family to be shown its abundance was ≥3% in at least one sample; bacterial families whose abundance was below this criterion are grouped in the category ‘rare’.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4530874&req=5

pone.0134643.g002: Bacterial Families.A histogram showing the relative proportion of different bacterial families present in 39 samples from 14 mice, where the two letter prefix denotes the location from where the mouse was captured and the three digit number is the unique mouse identifier; C and R are caecal and rectal samples, respectively, all as Table 1. For a bacterial family to be shown its abundance was ≥3% in at least one sample; bacterial families whose abundance was below this criterion are grouped in the category ‘rare’.
Mentions: At the bacterial family level, Lachnospiraceae and Ruminococcaceae (both Firmicutes) were the most common (mean abundance of 47% (SD 15%) and 15% (SD 11%), respectively) (Fig 2). Taxa of these families are associated with the maintenance of gut health and, while they contain functionally diverse taxa, they share a common role as active degraders of plant-derived material in the gut [56]. Our identification of OTUs by sequence homology to family level was very high, consistent with previous work showing that unknown or poorly described microbes in wild house mice are comparatively rare [35].

Bottom Line: The gut microbiota profoundly affects the biology of its host.The composition of the microbiota is dynamic and is affected by both host genetic and many environmental effects.In this study we have characterised the gut (both the caecum and rectum) microbiota of wild caught Mus musculus domesticus at three UK sites and have investigated how the microbiota varies depending on host location and host characteristics.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, University of Bristol, Bristol, United Kingdom.

ABSTRACT
The gut microbiota profoundly affects the biology of its host. The composition of the microbiota is dynamic and is affected by both host genetic and many environmental effects. The gut microbiota of laboratory mice has been studied extensively, which has uncovered many of the effects that the microbiota can have. This work has also shown that the environments of different research institutions can affect the mouse microbiota. There has been relatively limited study of the microbiota of wild mice, but this has shown that it typically differs from that of laboratory mice (and that maintaining wild caught mice in the laboratory can quite quickly alter the microbiota). There is also inter-individual variation in the microbiota of wild mice, with this principally explained by geographical location. In this study we have characterised the gut (both the caecum and rectum) microbiota of wild caught Mus musculus domesticus at three UK sites and have investigated how the microbiota varies depending on host location and host characteristics. We find that the microbiota of these mice are generally consistent with those described from other wild mice. The rectal and caecal microbiotas of individual mice are generally more similar to each other, than they are to the microbiota of other individuals. We found significant differences in the diversity of the microbiotas among mice from different sample sites. There were significant correlations of microbiota diversity and body weight, a measure of age, body-mass index, serum concentration of leptin, and virus, nematode and mite infection.

No MeSH data available.


Related in: MedlinePlus