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HIV-1 Dual Infected LTNP-EC Patients Developed an Unexpected Antibody Cross-Neutralizing Activity.

Pernas M, Sanchez-Merino V, Casado C, Merino-Mansilla A, Olivares I, Yuste E, Lopez-Galindez C - PLoS ONE (2015)

Bottom Line: Our results showed an improved neutralization breadth in DI LTNP-EC patients when compared with matched LTNP single-infected patients.We found a positive correlation between neutralization breadth and diversity within the viral quasispecies.This correlation could explain why a group of LTNP-EC patients developed a broad neutralizing response despite having undetectable levels of viremia.

View Article: PubMed Central - PubMed

Affiliation: Centro Nacional de Microbiología (CNM), Instituto de Salud Carlos III, Majadahonda, Madrid 28220, Spain.

ABSTRACT
This study evaluated the neutralization breadth in dually infected (DI) HIV-1 long-term non-progressor elite controller patients (LTNP-EC) using a representative minipanel of 6 viruses from 5 different subtypes. Our results showed an improved neutralization breadth in DI LTNP-EC patients when compared with matched LTNP single-infected patients. The role of viral diversity in neutralization was estimated with the Shannon Entropy and the p-distance in viral quasispecies. We found a positive correlation between neutralization breadth and diversity within the viral quasispecies. This correlation could explain why a group of LTNP-EC patients developed a broad neutralizing response despite having undetectable levels of viremia.

No MeSH data available.


Related in: MedlinePlus

Clinical parameters and neutralization breadth comparison between Dual vs Single-infected patients.Six different viruses form 5 different subtypes were used (VI191 subtype A, two subtype B viruses NL4.3 and AC10, 92BR025 subtype C, 92UG024 subtype D, CM244 subtype E). In brackets is represented the subtype and the tier. Numbers indicate the percentage of reduction in infectivity ± SD after neutralization. Neutralization levels are indicated as follows: black square >90%, dark grey square 90–70%, light grey square 70–50% and white square <50% reduction in infectivity. Number of cross-reactive HIV-1-subtypes neutralized by two samples plasma from each patient are represented. Median of cross-reactive HIV-1-neutralizing activity by dual and single infected patient was compared. A statistical analysis was performed with the non-parametric a 2-tailed Mann-Whitney U test with a restrictive significance at the 95% using GraphPad Prism V 4.0 software.
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pone.0134054.g002: Clinical parameters and neutralization breadth comparison between Dual vs Single-infected patients.Six different viruses form 5 different subtypes were used (VI191 subtype A, two subtype B viruses NL4.3 and AC10, 92BR025 subtype C, 92UG024 subtype D, CM244 subtype E). In brackets is represented the subtype and the tier. Numbers indicate the percentage of reduction in infectivity ± SD after neutralization. Neutralization levels are indicated as follows: black square >90%, dark grey square 90–70%, light grey square 70–50% and white square <50% reduction in infectivity. Number of cross-reactive HIV-1-subtypes neutralized by two samples plasma from each patient are represented. Median of cross-reactive HIV-1-neutralizing activity by dual and single infected patient was compared. A statistical analysis was performed with the non-parametric a 2-tailed Mann-Whitney U test with a restrictive significance at the 95% using GraphPad Prism V 4.0 software.

Mentions: To investigate the consequences of DI in LTNP neutralization breadth, we compared 3 DI versus 5 single-infected LTNP-EC patients (Fig 2). DI and single-infected LTNP groups were matched according to years after HIV-1 diagnosis, viral loads and T CD4+ cell counts (Data shown in Table 1). No statistical differences in the clinical parameters were observed between both groups.


HIV-1 Dual Infected LTNP-EC Patients Developed an Unexpected Antibody Cross-Neutralizing Activity.

Pernas M, Sanchez-Merino V, Casado C, Merino-Mansilla A, Olivares I, Yuste E, Lopez-Galindez C - PLoS ONE (2015)

Clinical parameters and neutralization breadth comparison between Dual vs Single-infected patients.Six different viruses form 5 different subtypes were used (VI191 subtype A, two subtype B viruses NL4.3 and AC10, 92BR025 subtype C, 92UG024 subtype D, CM244 subtype E). In brackets is represented the subtype and the tier. Numbers indicate the percentage of reduction in infectivity ± SD after neutralization. Neutralization levels are indicated as follows: black square >90%, dark grey square 90–70%, light grey square 70–50% and white square <50% reduction in infectivity. Number of cross-reactive HIV-1-subtypes neutralized by two samples plasma from each patient are represented. Median of cross-reactive HIV-1-neutralizing activity by dual and single infected patient was compared. A statistical analysis was performed with the non-parametric a 2-tailed Mann-Whitney U test with a restrictive significance at the 95% using GraphPad Prism V 4.0 software.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4530867&req=5

pone.0134054.g002: Clinical parameters and neutralization breadth comparison between Dual vs Single-infected patients.Six different viruses form 5 different subtypes were used (VI191 subtype A, two subtype B viruses NL4.3 and AC10, 92BR025 subtype C, 92UG024 subtype D, CM244 subtype E). In brackets is represented the subtype and the tier. Numbers indicate the percentage of reduction in infectivity ± SD after neutralization. Neutralization levels are indicated as follows: black square >90%, dark grey square 90–70%, light grey square 70–50% and white square <50% reduction in infectivity. Number of cross-reactive HIV-1-subtypes neutralized by two samples plasma from each patient are represented. Median of cross-reactive HIV-1-neutralizing activity by dual and single infected patient was compared. A statistical analysis was performed with the non-parametric a 2-tailed Mann-Whitney U test with a restrictive significance at the 95% using GraphPad Prism V 4.0 software.
Mentions: To investigate the consequences of DI in LTNP neutralization breadth, we compared 3 DI versus 5 single-infected LTNP-EC patients (Fig 2). DI and single-infected LTNP groups were matched according to years after HIV-1 diagnosis, viral loads and T CD4+ cell counts (Data shown in Table 1). No statistical differences in the clinical parameters were observed between both groups.

Bottom Line: Our results showed an improved neutralization breadth in DI LTNP-EC patients when compared with matched LTNP single-infected patients.We found a positive correlation between neutralization breadth and diversity within the viral quasispecies.This correlation could explain why a group of LTNP-EC patients developed a broad neutralizing response despite having undetectable levels of viremia.

View Article: PubMed Central - PubMed

Affiliation: Centro Nacional de Microbiología (CNM), Instituto de Salud Carlos III, Majadahonda, Madrid 28220, Spain.

ABSTRACT
This study evaluated the neutralization breadth in dually infected (DI) HIV-1 long-term non-progressor elite controller patients (LTNP-EC) using a representative minipanel of 6 viruses from 5 different subtypes. Our results showed an improved neutralization breadth in DI LTNP-EC patients when compared with matched LTNP single-infected patients. The role of viral diversity in neutralization was estimated with the Shannon Entropy and the p-distance in viral quasispecies. We found a positive correlation between neutralization breadth and diversity within the viral quasispecies. This correlation could explain why a group of LTNP-EC patients developed a broad neutralizing response despite having undetectable levels of viremia.

No MeSH data available.


Related in: MedlinePlus