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Strategies for Coordination of a Serosurvey in Parallel with an Immunization Coverage Survey.

Travassos MA, Beyene B, Adam Z, Campbell JD, Mulholland N, Diarra SS, Kassa T, Oot L, Sequeira J, Reymann M, Blackwelder WC, Pasetti MF, Sow SO, Steinglass R, Kebede A, Levine MM - Am. J. Trop. Med. Hyg. (2015)

Bottom Line: A community-based immunization coverage survey is the standard way to estimate effective vaccination delivery to a target population in a region.Accompanying serosurveys can provide objective measures of protective immunity against vaccine-preventable diseases but pose considerable challenges with respect to specimen collection and preservation and community compliance.Critical to the success of this effort were serosurvey equipment and supplies, team composition, and tight coordination with the coverage survey.

View Article: PubMed Central - PubMed

Affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Ethiopian Public Health Institute, Addis Ababa, Ethiopia; JSI Research and Training Institute Inc., Arlington, Virginia; Department of Social Work, The Women's Hospital, Melbourne, Australia; Centre pour le Développement des Vaccins, Bamako, Mali mtravass@medicine.umaryland.edu.

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Serum samples were centrifuged on-site within 4 hours using a portable centrifuge (Portafuge™) that plugged into the field vehicle.
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Figure 4: Serum samples were centrifuged on-site within 4 hours using a portable centrifuge (Portafuge™) that plugged into the field vehicle.

Mentions: Venous blood was drawn either directly into a serum separator tube (SST) or into a syringe that was then used to fill an SST. When centrifuged, the SST assures that serum is physically separated from the clot by a gel layer. This means that when the centrifuged tubes are put into a refrigerated transport box, should cold hemolysis of erythrocytes occur (as happens in a proportion of refrigerated clot specimens), the serum remains separated from the hemolyzed erythrocyte fragments. One member of the serosurvey team centrifuged the serum separator tubes on-site within 4 hours using a portable centrifuge (Portafuge™) that plugged into the field vehicle (Figure 4Figure 4.


Strategies for Coordination of a Serosurvey in Parallel with an Immunization Coverage Survey.

Travassos MA, Beyene B, Adam Z, Campbell JD, Mulholland N, Diarra SS, Kassa T, Oot L, Sequeira J, Reymann M, Blackwelder WC, Pasetti MF, Sow SO, Steinglass R, Kebede A, Levine MM - Am. J. Trop. Med. Hyg. (2015)

Serum samples were centrifuged on-site within 4 hours using a portable centrifuge (Portafuge™) that plugged into the field vehicle.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4530774&req=5

Figure 4: Serum samples were centrifuged on-site within 4 hours using a portable centrifuge (Portafuge™) that plugged into the field vehicle.
Mentions: Venous blood was drawn either directly into a serum separator tube (SST) or into a syringe that was then used to fill an SST. When centrifuged, the SST assures that serum is physically separated from the clot by a gel layer. This means that when the centrifuged tubes are put into a refrigerated transport box, should cold hemolysis of erythrocytes occur (as happens in a proportion of refrigerated clot specimens), the serum remains separated from the hemolyzed erythrocyte fragments. One member of the serosurvey team centrifuged the serum separator tubes on-site within 4 hours using a portable centrifuge (Portafuge™) that plugged into the field vehicle (Figure 4Figure 4.

Bottom Line: A community-based immunization coverage survey is the standard way to estimate effective vaccination delivery to a target population in a region.Accompanying serosurveys can provide objective measures of protective immunity against vaccine-preventable diseases but pose considerable challenges with respect to specimen collection and preservation and community compliance.Critical to the success of this effort were serosurvey equipment and supplies, team composition, and tight coordination with the coverage survey.

View Article: PubMed Central - PubMed

Affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Ethiopian Public Health Institute, Addis Ababa, Ethiopia; JSI Research and Training Institute Inc., Arlington, Virginia; Department of Social Work, The Women's Hospital, Melbourne, Australia; Centre pour le Développement des Vaccins, Bamako, Mali mtravass@medicine.umaryland.edu.

Show MeSH