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Strategies for Coordination of a Serosurvey in Parallel with an Immunization Coverage Survey.

Travassos MA, Beyene B, Adam Z, Campbell JD, Mulholland N, Diarra SS, Kassa T, Oot L, Sequeira J, Reymann M, Blackwelder WC, Pasetti MF, Sow SO, Steinglass R, Kebede A, Levine MM - Am. J. Trop. Med. Hyg. (2015)

Bottom Line: A community-based immunization coverage survey is the standard way to estimate effective vaccination delivery to a target population in a region.Accompanying serosurveys can provide objective measures of protective immunity against vaccine-preventable diseases but pose considerable challenges with respect to specimen collection and preservation and community compliance.Critical to the success of this effort were serosurvey equipment and supplies, team composition, and tight coordination with the coverage survey.

View Article: PubMed Central - PubMed

Affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Ethiopian Public Health Institute, Addis Ababa, Ethiopia; JSI Research and Training Institute Inc., Arlington, Virginia; Department of Social Work, The Women's Hospital, Melbourne, Australia; Centre pour le Développement des Vaccins, Bamako, Mali mtravass@medicine.umaryland.edu.

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Hemoglobin measurement and filter paper collection. For each subject, 10 μL blood was used to fill a cuvette for point-of-care hemoglobin measurement with a HemoCue® device, seen at top. Additional drops of blood were used to blot filter paper, completely filling each filter paper circle, seen at bottom.
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Figure 3: Hemoglobin measurement and filter paper collection. For each subject, 10 μL blood was used to fill a cuvette for point-of-care hemoglobin measurement with a HemoCue® device, seen at top. Additional drops of blood were used to blot filter paper, completely filling each filter paper circle, seen at bottom.

Mentions: A venous blood sample (maximum volume 3.5 mL) was drawn from each participant. Venipuncture was performed by an Ethiopian Public Health Institute (EPHI) phlebotomist with pediatric experience. The local health worker and team leader assisted with each blood draw, securing the child and assisting in the sample collection and processing. While papooses were available, the only tenable means for drawing blood from a child was found to be a parent holding the child with assistance from the serosurvey team. When a venous blood draw was unsuccessful, a fingerprick was obtained. Ten microliters of blood were used to fill a cuvette for point-of-care hemoglobin measurement. Additional drops of blood were used to blot filter paper, completely filling each filter paper circle, with up to five circles filled for each subject (Figure 3Figure 3.


Strategies for Coordination of a Serosurvey in Parallel with an Immunization Coverage Survey.

Travassos MA, Beyene B, Adam Z, Campbell JD, Mulholland N, Diarra SS, Kassa T, Oot L, Sequeira J, Reymann M, Blackwelder WC, Pasetti MF, Sow SO, Steinglass R, Kebede A, Levine MM - Am. J. Trop. Med. Hyg. (2015)

Hemoglobin measurement and filter paper collection. For each subject, 10 μL blood was used to fill a cuvette for point-of-care hemoglobin measurement with a HemoCue® device, seen at top. Additional drops of blood were used to blot filter paper, completely filling each filter paper circle, seen at bottom.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4530774&req=5

Figure 3: Hemoglobin measurement and filter paper collection. For each subject, 10 μL blood was used to fill a cuvette for point-of-care hemoglobin measurement with a HemoCue® device, seen at top. Additional drops of blood were used to blot filter paper, completely filling each filter paper circle, seen at bottom.
Mentions: A venous blood sample (maximum volume 3.5 mL) was drawn from each participant. Venipuncture was performed by an Ethiopian Public Health Institute (EPHI) phlebotomist with pediatric experience. The local health worker and team leader assisted with each blood draw, securing the child and assisting in the sample collection and processing. While papooses were available, the only tenable means for drawing blood from a child was found to be a parent holding the child with assistance from the serosurvey team. When a venous blood draw was unsuccessful, a fingerprick was obtained. Ten microliters of blood were used to fill a cuvette for point-of-care hemoglobin measurement. Additional drops of blood were used to blot filter paper, completely filling each filter paper circle, with up to five circles filled for each subject (Figure 3Figure 3.

Bottom Line: A community-based immunization coverage survey is the standard way to estimate effective vaccination delivery to a target population in a region.Accompanying serosurveys can provide objective measures of protective immunity against vaccine-preventable diseases but pose considerable challenges with respect to specimen collection and preservation and community compliance.Critical to the success of this effort were serosurvey equipment and supplies, team composition, and tight coordination with the coverage survey.

View Article: PubMed Central - PubMed

Affiliation: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Ethiopian Public Health Institute, Addis Ababa, Ethiopia; JSI Research and Training Institute Inc., Arlington, Virginia; Department of Social Work, The Women's Hospital, Melbourne, Australia; Centre pour le Développement des Vaccins, Bamako, Mali mtravass@medicine.umaryland.edu.

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