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Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceumATCC 12472.

Baraúna RA, Santos AV, Graças DA, Santos DM, Ghilardi R, Pimenta AM, Carepo MS, Schneider MP, Silva A - Genet. Mol. Biol. (2015)

Bottom Line: Several studies of the physiological responses of different organisms exposed to extremely low-frequency electromagnetic fields (ELF-EMF) have been described.The enhanced expression of Dps possibly helped to prevent physical damage to DNA.Although small, the changes in protein expression observed here were probably beneficial in helping the bacteria to adapt to the stress generated by the electromagnetic field.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Polimorfismo de DNA, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.

ABSTRACT
Several studies of the physiological responses of different organisms exposed to extremely low-frequency electromagnetic fields (ELF-EMF) have been described. In this work, we report the minimal effects of in situ exposure to ELF-EMF on the global protein expression of Chromobacterium violaceum using a gel-based proteomic approach. The protein expression profile was only slightly altered, with five differentially expressed proteins detected in the exposed cultures; two of these proteins (DNA-binding stress protein, Dps, and alcohol dehydrogenase) were identified by MS/MS. The enhanced expression of Dps possibly helped to prevent physical damage to DNA. Although small, the changes in protein expression observed here were probably beneficial in helping the bacteria to adapt to the stress generated by the electromagnetic field.

No MeSH data available.


Related in: MedlinePlus

2D-DIGE gel of C. violaceum bacteria exposed to a low frequency electromagnetic field. The differential spots are indicated by their Match ID. Spot 325 was down-regulated while the others were up-regulated. The scale above the gel indicates the pH range used in the first dimension of 2D-DIGE.
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f01: 2D-DIGE gel of C. violaceum bacteria exposed to a low frequency electromagnetic field. The differential spots are indicated by their Match ID. Spot 325 was down-regulated while the others were up-regulated. The scale above the gel indicates the pH range used in the first dimension of 2D-DIGE.

Mentions: The proteomic analysis detected five spots that were differentially expressed in the cultures near the breaker. Of these, one spot was down-regulated and four were up-regulated (Figure 1 and Table 1); two of these five spots were identified by MS/MS as DNA-binding stress protein (Dps) and alcohol dehydrogenase (Table 1 and Figure S1). These results showed that the exposure of C. violaceum to ELF-EMF under the conditions described here caused minimal changes in the bacterial protein expression profile. The two proteins identified here were related to DNA protection and cellular metabolism.


Exposure to an extremely low-frequency electromagnetic field only slightly modifies the proteome of Chromobacterium violaceumATCC 12472.

Baraúna RA, Santos AV, Graças DA, Santos DM, Ghilardi R, Pimenta AM, Carepo MS, Schneider MP, Silva A - Genet. Mol. Biol. (2015)

2D-DIGE gel of C. violaceum bacteria exposed to a low frequency electromagnetic field. The differential spots are indicated by their Match ID. Spot 325 was down-regulated while the others were up-regulated. The scale above the gel indicates the pH range used in the first dimension of 2D-DIGE.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4530650&req=5

f01: 2D-DIGE gel of C. violaceum bacteria exposed to a low frequency electromagnetic field. The differential spots are indicated by their Match ID. Spot 325 was down-regulated while the others were up-regulated. The scale above the gel indicates the pH range used in the first dimension of 2D-DIGE.
Mentions: The proteomic analysis detected five spots that were differentially expressed in the cultures near the breaker. Of these, one spot was down-regulated and four were up-regulated (Figure 1 and Table 1); two of these five spots were identified by MS/MS as DNA-binding stress protein (Dps) and alcohol dehydrogenase (Table 1 and Figure S1). These results showed that the exposure of C. violaceum to ELF-EMF under the conditions described here caused minimal changes in the bacterial protein expression profile. The two proteins identified here were related to DNA protection and cellular metabolism.

Bottom Line: Several studies of the physiological responses of different organisms exposed to extremely low-frequency electromagnetic fields (ELF-EMF) have been described.The enhanced expression of Dps possibly helped to prevent physical damage to DNA.Although small, the changes in protein expression observed here were probably beneficial in helping the bacteria to adapt to the stress generated by the electromagnetic field.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Polimorfismo de DNA, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.

ABSTRACT
Several studies of the physiological responses of different organisms exposed to extremely low-frequency electromagnetic fields (ELF-EMF) have been described. In this work, we report the minimal effects of in situ exposure to ELF-EMF on the global protein expression of Chromobacterium violaceum using a gel-based proteomic approach. The protein expression profile was only slightly altered, with five differentially expressed proteins detected in the exposed cultures; two of these proteins (DNA-binding stress protein, Dps, and alcohol dehydrogenase) were identified by MS/MS. The enhanced expression of Dps possibly helped to prevent physical damage to DNA. Although small, the changes in protein expression observed here were probably beneficial in helping the bacteria to adapt to the stress generated by the electromagnetic field.

No MeSH data available.


Related in: MedlinePlus