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Intrauterine Candida albicans infection elicits severe inflammation in fetal sheep.

Payne MS, Kemp MW, Kallapur SG, Kannan PS, Saito M, Miura Y, Newnham JP, Stock S, Ireland DJ, Kramer BW, Jobe AH - Pediatr. Res. (2014)

Bottom Line: Colonization of the amniotic cavity by C. albicans resulted in a modest inflammatory response at 1 d and florid inflammation at 2 d, characterized by fetal thrombocytopenia, lymphopenia, and significant increases of inflammatory cytokines/chemokines in the fetal membranes skin, lung, and the amniotic fluid.Acute colonization of the amniotic cavity by C. albicans causes severe intrauterine inflammation and fetal injury.C. albicans is a potent fetal pathogen that can contribute to adverse pregnancy outcomes.

View Article: PubMed Central - PubMed

Affiliation: School of Women's and Infants' Health, The University of Western Australia, Perth, Australia.

ABSTRACT

Background: Preventing preterm birth and subsequent adverse neonatal sequelae is among the greatest clinical challenges of our time. Recent studies suggest a role for Candida spp. in preterm birth and fetal injury, as a result of their colonization of either the vagina and/or the amniotic cavity. We hypothesized that intraamniotic Candida albicans would cause a vigorous, acute fetal inflammatory response.

Methods: Sheep carrying singleton pregnancies received single intraamniotic injections of either saline (control) or 10(7) colony-forming units C. albicans 1 or 2 d prior to surgical delivery and euthanasia at 124 ± 2 d gestation.

Results: Colonization of the amniotic cavity by C. albicans resulted in a modest inflammatory response at 1 d and florid inflammation at 2 d, characterized by fetal thrombocytopenia, lymphopenia, and significant increases of inflammatory cytokines/chemokines in the fetal membranes skin, lung, and the amniotic fluid.

Conclusion: Acute colonization of the amniotic cavity by C. albicans causes severe intrauterine inflammation and fetal injury. C. albicans is a potent fetal pathogen that can contribute to adverse pregnancy outcomes.

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Related in: MedlinePlus

Concentration of fetal arterial plasma cortisol (Control n=13; 1 d Candida n=8; 2 d Candida n=8) is increased in response to 2 d C. albicans exposure, vs. control. * p<0.05 vs. control; † p<0.05 vs. 1 d C. albicans exposure.
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Figure 2: Concentration of fetal arterial plasma cortisol (Control n=13; 1 d Candida n=8; 2 d Candida n=8) is increased in response to 2 d C. albicans exposure, vs. control. * p<0.05 vs. control; † p<0.05 vs. 1 d C. albicans exposure.

Mentions: Concentrations of cortisol in fetal arterial plasma demonstrated variable, but significant increases in concentration at 2 d post-infection relative to 1 d post-infection (p=0.007) and control (p=0.002) (Figure 2).


Intrauterine Candida albicans infection elicits severe inflammation in fetal sheep.

Payne MS, Kemp MW, Kallapur SG, Kannan PS, Saito M, Miura Y, Newnham JP, Stock S, Ireland DJ, Kramer BW, Jobe AH - Pediatr. Res. (2014)

Concentration of fetal arterial plasma cortisol (Control n=13; 1 d Candida n=8; 2 d Candida n=8) is increased in response to 2 d C. albicans exposure, vs. control. * p<0.05 vs. control; † p<0.05 vs. 1 d C. albicans exposure.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4530618&req=5

Figure 2: Concentration of fetal arterial plasma cortisol (Control n=13; 1 d Candida n=8; 2 d Candida n=8) is increased in response to 2 d C. albicans exposure, vs. control. * p<0.05 vs. control; † p<0.05 vs. 1 d C. albicans exposure.
Mentions: Concentrations of cortisol in fetal arterial plasma demonstrated variable, but significant increases in concentration at 2 d post-infection relative to 1 d post-infection (p=0.007) and control (p=0.002) (Figure 2).

Bottom Line: Colonization of the amniotic cavity by C. albicans resulted in a modest inflammatory response at 1 d and florid inflammation at 2 d, characterized by fetal thrombocytopenia, lymphopenia, and significant increases of inflammatory cytokines/chemokines in the fetal membranes skin, lung, and the amniotic fluid.Acute colonization of the amniotic cavity by C. albicans causes severe intrauterine inflammation and fetal injury.C. albicans is a potent fetal pathogen that can contribute to adverse pregnancy outcomes.

View Article: PubMed Central - PubMed

Affiliation: School of Women's and Infants' Health, The University of Western Australia, Perth, Australia.

ABSTRACT

Background: Preventing preterm birth and subsequent adverse neonatal sequelae is among the greatest clinical challenges of our time. Recent studies suggest a role for Candida spp. in preterm birth and fetal injury, as a result of their colonization of either the vagina and/or the amniotic cavity. We hypothesized that intraamniotic Candida albicans would cause a vigorous, acute fetal inflammatory response.

Methods: Sheep carrying singleton pregnancies received single intraamniotic injections of either saline (control) or 10(7) colony-forming units C. albicans 1 or 2 d prior to surgical delivery and euthanasia at 124 ± 2 d gestation.

Results: Colonization of the amniotic cavity by C. albicans resulted in a modest inflammatory response at 1 d and florid inflammation at 2 d, characterized by fetal thrombocytopenia, lymphopenia, and significant increases of inflammatory cytokines/chemokines in the fetal membranes skin, lung, and the amniotic fluid.

Conclusion: Acute colonization of the amniotic cavity by C. albicans causes severe intrauterine inflammation and fetal injury. C. albicans is a potent fetal pathogen that can contribute to adverse pregnancy outcomes.

Show MeSH
Related in: MedlinePlus