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STIL binding to Polo-box 3 of PLK4 regulates centriole duplication.

Arquint C, Gabryjonczyk AM, Imseng S, Böhm R, Sauer E, Hiller S, Nigg EA, Maier T - Elife (2015)

Bottom Line: STIL-CC is the first identified interaction partner of Polo-box 3 (PB3) of PLK4 and also uses a secondary interaction site in the PLK4 L1 region.In vivo analysis of structure-guided STIL mutants reveals distinct binding modes to PLK4-PB3 and L1, as well as interplay of STIL oligomerization with PLK4 binding.We suggest that the STIL-CC/PLK4 interaction mediates PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplication.

View Article: PubMed Central - PubMed

Affiliation: Biozentrum, University of Basel, Basel, Switzerland.

ABSTRACT
Polo-like kinases (PLK) are eukaryotic regulators of cell cycle progression, mitosis and cytokinesis; PLK4 is a master regulator of centriole duplication. Here, we demonstrate that the SCL/TAL1 interrupting locus (STIL) protein interacts via its coiled-coil region (STIL-CC) with PLK4 in vivo. STIL-CC is the first identified interaction partner of Polo-box 3 (PB3) of PLK4 and also uses a secondary interaction site in the PLK4 L1 region. Structure determination of free PLK4-PB3 and its STIL-CC complex via NMR and crystallography reveals a novel mode of Polo-box-peptide interaction mimicking coiled-coil formation. In vivo analysis of structure-guided STIL mutants reveals distinct binding modes to PLK4-PB3 and L1, as well as interplay of STIL oligomerization with PLK4 binding. We suggest that the STIL-CC/PLK4 interaction mediates PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplication.

No MeSH data available.


Related in: MedlinePlus

STIL–CC binding to PLK4-PB3 mimics coiled-coil interactions.Close-up views of the STIL-CC/PLK4-PB3 binding interface. Key contributing residues to the interaction between PLK4-PB3 (light blue) and STIL-CC (green) are indicated.DOI:http://dx.doi.org/10.7554/eLife.07888.017
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fig6s1: STIL–CC binding to PLK4-PB3 mimics coiled-coil interactions.Close-up views of the STIL-CC/PLK4-PB3 binding interface. Key contributing residues to the interaction between PLK4-PB3 (light blue) and STIL-CC (green) are indicated.DOI:http://dx.doi.org/10.7554/eLife.07888.017

Mentions: PLK4-PB3 and STIL-CC interact along the entire STIL-helix and form a substantial interface of 934 Å2 buried surface area (Krissinel and Henrick, 2007). Two regions on PLK4-PB3 contribute to the predominantly hydrophobic binding interface: First, the surface of the PLK4-PB3 β-sheet around residues V907, L917, V919, I926 and Y928 (Figure 6—figure supplement 1), and second the α1 helix (I948, L952, L955 and L959) and the linker (L944) leading into it (Figure 6A). Key interacting residues on STIL-CC are leucine and isoleucine residues (L733, L736, I740, L743, L744) pointing towards the hydrophobic surface of PLK4-PB3. Additional interactions are provided by backbone–backbone hydrogen bonds between PB3G922-STILQ739 and PB3K943-STILM750 (Figure 6—figure supplement 1), respectively. The orientation of the two helices and their hydrophobic interactions is mainly mediated by leucine residues and resembles a leucine zipper interaction, consistent with the predicted CC propensity of STIL-CC (Stevens et al., 2010).10.7554/eLife.07888.016Figure 6.Analysis of structure-based STIL-CC mutants.


STIL binding to Polo-box 3 of PLK4 regulates centriole duplication.

Arquint C, Gabryjonczyk AM, Imseng S, Böhm R, Sauer E, Hiller S, Nigg EA, Maier T - Elife (2015)

STIL–CC binding to PLK4-PB3 mimics coiled-coil interactions.Close-up views of the STIL-CC/PLK4-PB3 binding interface. Key contributing residues to the interaction between PLK4-PB3 (light blue) and STIL-CC (green) are indicated.DOI:http://dx.doi.org/10.7554/eLife.07888.017
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4530586&req=5

fig6s1: STIL–CC binding to PLK4-PB3 mimics coiled-coil interactions.Close-up views of the STIL-CC/PLK4-PB3 binding interface. Key contributing residues to the interaction between PLK4-PB3 (light blue) and STIL-CC (green) are indicated.DOI:http://dx.doi.org/10.7554/eLife.07888.017
Mentions: PLK4-PB3 and STIL-CC interact along the entire STIL-helix and form a substantial interface of 934 Å2 buried surface area (Krissinel and Henrick, 2007). Two regions on PLK4-PB3 contribute to the predominantly hydrophobic binding interface: First, the surface of the PLK4-PB3 β-sheet around residues V907, L917, V919, I926 and Y928 (Figure 6—figure supplement 1), and second the α1 helix (I948, L952, L955 and L959) and the linker (L944) leading into it (Figure 6A). Key interacting residues on STIL-CC are leucine and isoleucine residues (L733, L736, I740, L743, L744) pointing towards the hydrophobic surface of PLK4-PB3. Additional interactions are provided by backbone–backbone hydrogen bonds between PB3G922-STILQ739 and PB3K943-STILM750 (Figure 6—figure supplement 1), respectively. The orientation of the two helices and their hydrophobic interactions is mainly mediated by leucine residues and resembles a leucine zipper interaction, consistent with the predicted CC propensity of STIL-CC (Stevens et al., 2010).10.7554/eLife.07888.016Figure 6.Analysis of structure-based STIL-CC mutants.

Bottom Line: STIL-CC is the first identified interaction partner of Polo-box 3 (PB3) of PLK4 and also uses a secondary interaction site in the PLK4 L1 region.In vivo analysis of structure-guided STIL mutants reveals distinct binding modes to PLK4-PB3 and L1, as well as interplay of STIL oligomerization with PLK4 binding.We suggest that the STIL-CC/PLK4 interaction mediates PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplication.

View Article: PubMed Central - PubMed

Affiliation: Biozentrum, University of Basel, Basel, Switzerland.

ABSTRACT
Polo-like kinases (PLK) are eukaryotic regulators of cell cycle progression, mitosis and cytokinesis; PLK4 is a master regulator of centriole duplication. Here, we demonstrate that the SCL/TAL1 interrupting locus (STIL) protein interacts via its coiled-coil region (STIL-CC) with PLK4 in vivo. STIL-CC is the first identified interaction partner of Polo-box 3 (PB3) of PLK4 and also uses a secondary interaction site in the PLK4 L1 region. Structure determination of free PLK4-PB3 and its STIL-CC complex via NMR and crystallography reveals a novel mode of Polo-box-peptide interaction mimicking coiled-coil formation. In vivo analysis of structure-guided STIL mutants reveals distinct binding modes to PLK4-PB3 and L1, as well as interplay of STIL oligomerization with PLK4 binding. We suggest that the STIL-CC/PLK4 interaction mediates PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplication.

No MeSH data available.


Related in: MedlinePlus