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Prenatal genesis of layer II doublecortin expressing neurons in neonatal and young adult guinea pig cerebral cortex.

Yang Y, Xie MX, Li JM, Hu X, Patrylo PR, Luo XG, Cai Y, Li Z, Yan XX - Front Neuroanat (2015)

Bottom Line: In the piriform cortex, cells generated at E21 and E28 occurred with a greater density in layer II than III.Many cells were generated at later time points until birth, occurring in the cortex without a laminar preference.DCX+ cells in the neocortex and piriform cortex partially co-colocalized with BrdU (up to 7.5%) in the newborns after pulse-chasing from E21 to E49 and in the 2 month-old animals after pulse-chasing from E28 to E60/61, with higher rates seen among the E21-E35 groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Neurobiology, Central South University School of Basic Medicine Changsha, China ; Department of Nursing in Internal Medicine, Xiangtan Vocational and Technical College Xiangtan, China.

ABSTRACT
Cells expressing doublecortin (DCX+) occur at cortical layer II, predominantly over the paleocortex in mice/rats, but also across the neocortex among larger mammals. Here, we explored the time of origin of these cells in neonatal and 2-month-old guinea pigs following prenatal BrdU pulse-chasing. In the neocortex, BrdU+ cells birth-dated at embryonic day 21 (E21), E28, and E35 laminated over the cortical plate with an inside-out order. In the piriform cortex, cells generated at E21 and E28 occurred with a greater density in layer II than III. Many cells were generated at later time points until birth, occurring in the cortex without a laminar preference. DCX+ cells in the neocortex and piriform cortex partially co-colocalized with BrdU (up to 7.5%) in the newborns after pulse-chasing from E21 to E49 and in the 2 month-old animals after pulse-chasing from E28 to E60/61, with higher rates seen among the E21-E35 groups. Together, layer II DCX+ cells in neonatal and young adult guinea pigs may be produced over a wide prenatal time window, but mainly during the early phases of corticogenesis. Our data also show an earlier establishment of the basic lamination in the piriform relative to neocortical areas in guinea pigs.

No MeSH data available.


Confocal immunofluorescent images showing colocalization of BrdU in layer II doublecortin expressing (DCX+) cells in the neocortex and piriform cortex in representative time groups as indicated. Examples of double-labeled cells are indicated by arrows and enlarged inserts in the panels (A–X) as labeled. BrdU immunoreactivity may be densely packed or appear as granules in the nucleus of single and double-labeled cells. The morphology and labeling intensity of DCX+ cells also show a great extent of variability. Scale bar = 50 μm in (A) applying for panels (B–X).
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Figure 5: Confocal immunofluorescent images showing colocalization of BrdU in layer II doublecortin expressing (DCX+) cells in the neocortex and piriform cortex in representative time groups as indicated. Examples of double-labeled cells are indicated by arrows and enlarged inserts in the panels (A–X) as labeled. BrdU immunoreactivity may be densely packed or appear as granules in the nucleus of single and double-labeled cells. The morphology and labeling intensity of DCX+ cells also show a great extent of variability. Scale bar = 50 μm in (A) applying for panels (B–X).

Mentions: In examination of double immunofluorescent sections we observed a partial BrdU colocalization among layer II DCX+ cells across the cortical hemisphere in most groups of the P0 and P60 offspring (Figure 5, showing images from representative groups). In a given brain, double-labeled cells could be detected in the parietotemporal, entorhinal, and piriform cortical areas. Consistent with previous observations (Xiong et al., 2008; Cai et al., 2009), layer II DCX+ cells exhibited a great extent of variability in morphology and labeling intensity. Among the BrdU+ cells including those coexpressing DCX, BrdU labeling in the nucleus appeared densely packed, or granular and punctate (Figures 5C,D,G,H,K,L,O,P; enlarged inserts).


Prenatal genesis of layer II doublecortin expressing neurons in neonatal and young adult guinea pig cerebral cortex.

Yang Y, Xie MX, Li JM, Hu X, Patrylo PR, Luo XG, Cai Y, Li Z, Yan XX - Front Neuroanat (2015)

Confocal immunofluorescent images showing colocalization of BrdU in layer II doublecortin expressing (DCX+) cells in the neocortex and piriform cortex in representative time groups as indicated. Examples of double-labeled cells are indicated by arrows and enlarged inserts in the panels (A–X) as labeled. BrdU immunoreactivity may be densely packed or appear as granules in the nucleus of single and double-labeled cells. The morphology and labeling intensity of DCX+ cells also show a great extent of variability. Scale bar = 50 μm in (A) applying for panels (B–X).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4530311&req=5

Figure 5: Confocal immunofluorescent images showing colocalization of BrdU in layer II doublecortin expressing (DCX+) cells in the neocortex and piriform cortex in representative time groups as indicated. Examples of double-labeled cells are indicated by arrows and enlarged inserts in the panels (A–X) as labeled. BrdU immunoreactivity may be densely packed or appear as granules in the nucleus of single and double-labeled cells. The morphology and labeling intensity of DCX+ cells also show a great extent of variability. Scale bar = 50 μm in (A) applying for panels (B–X).
Mentions: In examination of double immunofluorescent sections we observed a partial BrdU colocalization among layer II DCX+ cells across the cortical hemisphere in most groups of the P0 and P60 offspring (Figure 5, showing images from representative groups). In a given brain, double-labeled cells could be detected in the parietotemporal, entorhinal, and piriform cortical areas. Consistent with previous observations (Xiong et al., 2008; Cai et al., 2009), layer II DCX+ cells exhibited a great extent of variability in morphology and labeling intensity. Among the BrdU+ cells including those coexpressing DCX, BrdU labeling in the nucleus appeared densely packed, or granular and punctate (Figures 5C,D,G,H,K,L,O,P; enlarged inserts).

Bottom Line: In the piriform cortex, cells generated at E21 and E28 occurred with a greater density in layer II than III.Many cells were generated at later time points until birth, occurring in the cortex without a laminar preference.DCX+ cells in the neocortex and piriform cortex partially co-colocalized with BrdU (up to 7.5%) in the newborns after pulse-chasing from E21 to E49 and in the 2 month-old animals after pulse-chasing from E28 to E60/61, with higher rates seen among the E21-E35 groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy and Neurobiology, Central South University School of Basic Medicine Changsha, China ; Department of Nursing in Internal Medicine, Xiangtan Vocational and Technical College Xiangtan, China.

ABSTRACT
Cells expressing doublecortin (DCX+) occur at cortical layer II, predominantly over the paleocortex in mice/rats, but also across the neocortex among larger mammals. Here, we explored the time of origin of these cells in neonatal and 2-month-old guinea pigs following prenatal BrdU pulse-chasing. In the neocortex, BrdU+ cells birth-dated at embryonic day 21 (E21), E28, and E35 laminated over the cortical plate with an inside-out order. In the piriform cortex, cells generated at E21 and E28 occurred with a greater density in layer II than III. Many cells were generated at later time points until birth, occurring in the cortex without a laminar preference. DCX+ cells in the neocortex and piriform cortex partially co-colocalized with BrdU (up to 7.5%) in the newborns after pulse-chasing from E21 to E49 and in the 2 month-old animals after pulse-chasing from E28 to E60/61, with higher rates seen among the E21-E35 groups. Together, layer II DCX+ cells in neonatal and young adult guinea pigs may be produced over a wide prenatal time window, but mainly during the early phases of corticogenesis. Our data also show an earlier establishment of the basic lamination in the piriform relative to neocortical areas in guinea pigs.

No MeSH data available.