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Swertianlarin, an Herbal Agent Derived from Swertia mussotii Franch, Attenuates Liver Injury, Inflammation, and Cholestasis in Common Bile Duct-Ligated Rats.

Zhang L, Cheng Y, Du X, Chen S, Feng X, Gao Y, Li S, Liu L, Yang M, Chen L, Peng Z, Yang Y, Luo W, Wang R, Chen W, Chai J - Evid Based Complement Alternat Med (2015)

Bottom Line: Swertianlarin is an herbal agent abundantly distributed in Swertia mussotii Franch, a Chinese traditional herb used for treatment of jaundice.Moreover, reductions in serum interleukins IL-1β and IL-6 levels were also observed in BDL rats treated with swertianlarin (P < 0.05).In addition, most of serum toxic bile salt concentrations (e.g., chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA)) in cholestatic rats were decreased by swertianlarin (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
Swertianlarin is an herbal agent abundantly distributed in Swertia mussotii Franch, a Chinese traditional herb used for treatment of jaundice. To study the therapeutic effect of swertianlarin on cholestasis, liver injury, serum proinflammatory cytokines, and bile salt concentrations were measured by comparing rats treated with swertianlarin 100 mg/kg/d or saline for 3, 7, or 14 days after bile duct ligation (BDL). Serum alanine aminotransferase (ATL) and aspartate aminotransferase (AST) levels were significantly decreased in BDL rats treated with swertianlarin for 14 days (P < 0.05). The reduced liver injury in BDL rats by swertianlarin treatment for 14 days was further confirmed by liver histopathology. Levels of serum tumor necrosis factor alpha (TNFα) were decreased by swertianlarin in BDL rats for 3 and 7 days (P < 0.05). Moreover, reductions in serum interleukins IL-1β and IL-6 levels were also observed in BDL rats treated with swertianlarin (P < 0.05). In addition, most of serum toxic bile salt concentrations (e.g., chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA)) in cholestatic rats were decreased by swertianlarin (P < 0.05). In conclusion, the data suggest that swertianlarin derived from Swertia mussotii Franch attenuates liver injury, inflammation, and cholestasis in bile duct-ligated rats.

No MeSH data available.


Related in: MedlinePlus

Alterations of serum bile salts by swertianlarin in BDL rats. (a) The concentrations of serum CDCA and TCDCA in sham operated rats and BDL rats with or without swertianlarin for 3, 7, and 14 d. (b) The changes in serum bile salts CA and TCA in sham operated, BDL with swertianlarin, and BDL without swertianlarin groups for 3, 7, and 14 d. (c) The determination of serum DCA and TDCA in sham operated rats and BDL rats treated with or without swertianlarin after 3, 7, and 14 d. (d) Serum TUDCA, (e) Tα/βMCA, and (f) αMCA and βMCA were measured in sham operated group, BDL with swertianlarin group, and BDL without swertianlarin group for 3, 7, and 14 d. Data were analyzed as described in Materials and Methods. *P < 0.05 versus sham group with saline; #P < 0.05, versus BDL group with saline. n = 7 per group. Saline, 1% Tween-20 saline; swertianlarin dissolved in 1% Tween-20 saline.
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fig3: Alterations of serum bile salts by swertianlarin in BDL rats. (a) The concentrations of serum CDCA and TCDCA in sham operated rats and BDL rats with or without swertianlarin for 3, 7, and 14 d. (b) The changes in serum bile salts CA and TCA in sham operated, BDL with swertianlarin, and BDL without swertianlarin groups for 3, 7, and 14 d. (c) The determination of serum DCA and TDCA in sham operated rats and BDL rats treated with or without swertianlarin after 3, 7, and 14 d. (d) Serum TUDCA, (e) Tα/βMCA, and (f) αMCA and βMCA were measured in sham operated group, BDL with swertianlarin group, and BDL without swertianlarin group for 3, 7, and 14 d. Data were analyzed as described in Materials and Methods. *P < 0.05 versus sham group with saline; #P < 0.05, versus BDL group with saline. n = 7 per group. Saline, 1% Tween-20 saline; swertianlarin dissolved in 1% Tween-20 saline.

Mentions: To investigate whether swertianlarin has anti-inflammatory effects, the concentration of serum proinflammatory cytokines TNFα, IL-1β, and IL-6 were measured by ELISA assay in the sham operated group and BDL groups with or without swertianlarin. The results showed that the serum TNFα levels for 3 and 7 d were dramatically increased while they were not significantly increased for 14 d in cholestatic rats without swertianlarin compared to sham operated rats (Figure 2(a)). However, the levels of serum TNFα in cholestatic rats with BDL for 3 and 7 d were significantly lower with swertianlarin treatment, 63% and 64% of saline BDL group, respectively (P < 0.05). However, they were not significantly different for 14 d, compared to the BDL group without swertianlarin (Figure 2(a)). Furthermore, serum IL-1β levels were significantly increased for 14 d while they were unchanged for 3 and 7 d in the BDL group with swertianlarin compared to those of the sham operated group (Figure 2(b)). However, the serum IL-1β levels for 3 and 14 d were noticeably lower in the swertianlarin BDL group (43% and 42% of BDL group without swertianlarin, resp.; P < 0.05). Moreover, the serum IL-1β levels were not altered by swertianlarin for 7 d (Figure 2(b)). Figure 2(c) shows that serum IL-6 levels were increased for 14 d but was decreased for 3 d (P < 0.05) and was not different for 7 d in BDL group without swertianlarin compared to the sham operated group (Figure 3(c)). Serum IL-6 in the swertianlarin BDL group for 14 d was 61% of saline BDL group (P < 0.05), whereas the serum IL-6 levels were not affected by swertianlarin for 3 and 7 d (Figure 3(c)). These results demonstrated that swertianlarin treatment reduced levels of serum proinflammatory cytokines TNFα, IL-1β, and IL-6 in this rat model.


Swertianlarin, an Herbal Agent Derived from Swertia mussotii Franch, Attenuates Liver Injury, Inflammation, and Cholestasis in Common Bile Duct-Ligated Rats.

Zhang L, Cheng Y, Du X, Chen S, Feng X, Gao Y, Li S, Liu L, Yang M, Chen L, Peng Z, Yang Y, Luo W, Wang R, Chen W, Chai J - Evid Based Complement Alternat Med (2015)

Alterations of serum bile salts by swertianlarin in BDL rats. (a) The concentrations of serum CDCA and TCDCA in sham operated rats and BDL rats with or without swertianlarin for 3, 7, and 14 d. (b) The changes in serum bile salts CA and TCA in sham operated, BDL with swertianlarin, and BDL without swertianlarin groups for 3, 7, and 14 d. (c) The determination of serum DCA and TDCA in sham operated rats and BDL rats treated with or without swertianlarin after 3, 7, and 14 d. (d) Serum TUDCA, (e) Tα/βMCA, and (f) αMCA and βMCA were measured in sham operated group, BDL with swertianlarin group, and BDL without swertianlarin group for 3, 7, and 14 d. Data were analyzed as described in Materials and Methods. *P < 0.05 versus sham group with saline; #P < 0.05, versus BDL group with saline. n = 7 per group. Saline, 1% Tween-20 saline; swertianlarin dissolved in 1% Tween-20 saline.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4530240&req=5

fig3: Alterations of serum bile salts by swertianlarin in BDL rats. (a) The concentrations of serum CDCA and TCDCA in sham operated rats and BDL rats with or without swertianlarin for 3, 7, and 14 d. (b) The changes in serum bile salts CA and TCA in sham operated, BDL with swertianlarin, and BDL without swertianlarin groups for 3, 7, and 14 d. (c) The determination of serum DCA and TDCA in sham operated rats and BDL rats treated with or without swertianlarin after 3, 7, and 14 d. (d) Serum TUDCA, (e) Tα/βMCA, and (f) αMCA and βMCA were measured in sham operated group, BDL with swertianlarin group, and BDL without swertianlarin group for 3, 7, and 14 d. Data were analyzed as described in Materials and Methods. *P < 0.05 versus sham group with saline; #P < 0.05, versus BDL group with saline. n = 7 per group. Saline, 1% Tween-20 saline; swertianlarin dissolved in 1% Tween-20 saline.
Mentions: To investigate whether swertianlarin has anti-inflammatory effects, the concentration of serum proinflammatory cytokines TNFα, IL-1β, and IL-6 were measured by ELISA assay in the sham operated group and BDL groups with or without swertianlarin. The results showed that the serum TNFα levels for 3 and 7 d were dramatically increased while they were not significantly increased for 14 d in cholestatic rats without swertianlarin compared to sham operated rats (Figure 2(a)). However, the levels of serum TNFα in cholestatic rats with BDL for 3 and 7 d were significantly lower with swertianlarin treatment, 63% and 64% of saline BDL group, respectively (P < 0.05). However, they were not significantly different for 14 d, compared to the BDL group without swertianlarin (Figure 2(a)). Furthermore, serum IL-1β levels were significantly increased for 14 d while they were unchanged for 3 and 7 d in the BDL group with swertianlarin compared to those of the sham operated group (Figure 2(b)). However, the serum IL-1β levels for 3 and 14 d were noticeably lower in the swertianlarin BDL group (43% and 42% of BDL group without swertianlarin, resp.; P < 0.05). Moreover, the serum IL-1β levels were not altered by swertianlarin for 7 d (Figure 2(b)). Figure 2(c) shows that serum IL-6 levels were increased for 14 d but was decreased for 3 d (P < 0.05) and was not different for 7 d in BDL group without swertianlarin compared to the sham operated group (Figure 3(c)). Serum IL-6 in the swertianlarin BDL group for 14 d was 61% of saline BDL group (P < 0.05), whereas the serum IL-6 levels were not affected by swertianlarin for 3 and 7 d (Figure 3(c)). These results demonstrated that swertianlarin treatment reduced levels of serum proinflammatory cytokines TNFα, IL-1β, and IL-6 in this rat model.

Bottom Line: Swertianlarin is an herbal agent abundantly distributed in Swertia mussotii Franch, a Chinese traditional herb used for treatment of jaundice.Moreover, reductions in serum interleukins IL-1β and IL-6 levels were also observed in BDL rats treated with swertianlarin (P < 0.05).In addition, most of serum toxic bile salt concentrations (e.g., chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA)) in cholestatic rats were decreased by swertianlarin (P < 0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
Swertianlarin is an herbal agent abundantly distributed in Swertia mussotii Franch, a Chinese traditional herb used for treatment of jaundice. To study the therapeutic effect of swertianlarin on cholestasis, liver injury, serum proinflammatory cytokines, and bile salt concentrations were measured by comparing rats treated with swertianlarin 100 mg/kg/d or saline for 3, 7, or 14 days after bile duct ligation (BDL). Serum alanine aminotransferase (ATL) and aspartate aminotransferase (AST) levels were significantly decreased in BDL rats treated with swertianlarin for 14 days (P < 0.05). The reduced liver injury in BDL rats by swertianlarin treatment for 14 days was further confirmed by liver histopathology. Levels of serum tumor necrosis factor alpha (TNFα) were decreased by swertianlarin in BDL rats for 3 and 7 days (P < 0.05). Moreover, reductions in serum interleukins IL-1β and IL-6 levels were also observed in BDL rats treated with swertianlarin (P < 0.05). In addition, most of serum toxic bile salt concentrations (e.g., chenodeoxycholic acid (CDCA) and deoxycholic acid (DCA)) in cholestatic rats were decreased by swertianlarin (P < 0.05). In conclusion, the data suggest that swertianlarin derived from Swertia mussotii Franch attenuates liver injury, inflammation, and cholestasis in bile duct-ligated rats.

No MeSH data available.


Related in: MedlinePlus