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Zuogui Jiangtang Jieyu Formulation Prevents Hyperglycaemia and Depressive-Like Behaviour in Rats by Reducing the Glucocorticoid Level in Plasma and Hippocampus.

Wang Y, Yang H, Li W, Meng P, Han Y, Zhang X, Cao D, Tan Y - Evid Based Complement Alternat Med (2015)

Bottom Line: Results.We found that (1) the treatment with ZGJTJY (10.26 g/kg) increases the motor activities and improves cognition ability. (2) ZGJTJY (10.26 g/kg) significantly relieves the disorder in blood and the relative indexes. (3) ZGJTJY (10.26 g/kg) can reduce hippocampal corticosterone expression levels and further improve hippocampus pathological changes. (4) ZGJTJY increased the expression of GR accompanied with decreasing 11β-HSD1 in hippocampus.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Hunan University of Chinese Medicine, No. 300, Bachelor Road, Changsha, Hunan 410208, China.

ABSTRACT
Aim. To determine whether Zuogui Jiangtang Jieyu prescription (ZGJTJY) has hypoglycemic and antidepressant effects which are mediated by corticosterone through adjustment of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and glucocorticoid (GR) levels. Materials and Methods. The diabetes-related depression rats were randomly divided into four groups: the model group, metformin (1.8 mg/kg) combined with fluoxetine (10.8 mg/kg) group, and ZGJTJY high and low dose groups. Four weeks after modeling, blood glucose, behavior, and cognitive function of depression were detected. The expressions of 11β-HSD1 and GR in hippocampus were measured by western blotting and immunohistochemical experiments. Results. We found that (1) the treatment with ZGJTJY (10.26 g/kg) increases the motor activities and improves cognition ability. (2) ZGJTJY (10.26 g/kg) significantly relieves the disorder in blood and the relative indexes. (3) ZGJTJY (10.26 g/kg) can reduce hippocampal corticosterone expression levels and further improve hippocampus pathological changes. (4) ZGJTJY increased the expression of GR accompanied with decreasing 11β-HSD1 in hippocampus. Conclusions. ZGJTJY inhibits the expression of 11β-HSD1 and increases GR in hippocampus and subsequently modulates blood glucose levels, and therefore it is potential property that ZGJTJY could be of benefit for the treatment of behavior and cognitive function of diabetes-related depression.

No MeSH data available.


Related in: MedlinePlus

The expression of CORT was inhibited by treatment of ZGJTJY. The CORT expression in hippocampus was measured by ELISA kit following the manufacturer's protocol. *P < 0.05 compared with control; **P < 0.01 compared with control; #P < 0.05 compared with vehicle, ##P < 0.01 compared with vehicle; n = 8, .
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fig5: The expression of CORT was inhibited by treatment of ZGJTJY. The CORT expression in hippocampus was measured by ELISA kit following the manufacturer's protocol. *P < 0.05 compared with control; **P < 0.01 compared with control; #P < 0.05 compared with vehicle, ##P < 0.01 compared with vehicle; n = 8, .

Mentions: The content of CORT in hippocampus is presented in Figure 5.


Zuogui Jiangtang Jieyu Formulation Prevents Hyperglycaemia and Depressive-Like Behaviour in Rats by Reducing the Glucocorticoid Level in Plasma and Hippocampus.

Wang Y, Yang H, Li W, Meng P, Han Y, Zhang X, Cao D, Tan Y - Evid Based Complement Alternat Med (2015)

The expression of CORT was inhibited by treatment of ZGJTJY. The CORT expression in hippocampus was measured by ELISA kit following the manufacturer's protocol. *P < 0.05 compared with control; **P < 0.01 compared with control; #P < 0.05 compared with vehicle, ##P < 0.01 compared with vehicle; n = 8, .
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4530230&req=5

fig5: The expression of CORT was inhibited by treatment of ZGJTJY. The CORT expression in hippocampus was measured by ELISA kit following the manufacturer's protocol. *P < 0.05 compared with control; **P < 0.01 compared with control; #P < 0.05 compared with vehicle, ##P < 0.01 compared with vehicle; n = 8, .
Mentions: The content of CORT in hippocampus is presented in Figure 5.

Bottom Line: Results.We found that (1) the treatment with ZGJTJY (10.26 g/kg) increases the motor activities and improves cognition ability. (2) ZGJTJY (10.26 g/kg) significantly relieves the disorder in blood and the relative indexes. (3) ZGJTJY (10.26 g/kg) can reduce hippocampal corticosterone expression levels and further improve hippocampus pathological changes. (4) ZGJTJY increased the expression of GR accompanied with decreasing 11β-HSD1 in hippocampus.Conclusions.

View Article: PubMed Central - PubMed

Affiliation: Hunan University of Chinese Medicine, No. 300, Bachelor Road, Changsha, Hunan 410208, China.

ABSTRACT
Aim. To determine whether Zuogui Jiangtang Jieyu prescription (ZGJTJY) has hypoglycemic and antidepressant effects which are mediated by corticosterone through adjustment of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and glucocorticoid (GR) levels. Materials and Methods. The diabetes-related depression rats were randomly divided into four groups: the model group, metformin (1.8 mg/kg) combined with fluoxetine (10.8 mg/kg) group, and ZGJTJY high and low dose groups. Four weeks after modeling, blood glucose, behavior, and cognitive function of depression were detected. The expressions of 11β-HSD1 and GR in hippocampus were measured by western blotting and immunohistochemical experiments. Results. We found that (1) the treatment with ZGJTJY (10.26 g/kg) increases the motor activities and improves cognition ability. (2) ZGJTJY (10.26 g/kg) significantly relieves the disorder in blood and the relative indexes. (3) ZGJTJY (10.26 g/kg) can reduce hippocampal corticosterone expression levels and further improve hippocampus pathological changes. (4) ZGJTJY increased the expression of GR accompanied with decreasing 11β-HSD1 in hippocampus. Conclusions. ZGJTJY inhibits the expression of 11β-HSD1 and increases GR in hippocampus and subsequently modulates blood glucose levels, and therefore it is potential property that ZGJTJY could be of benefit for the treatment of behavior and cognitive function of diabetes-related depression.

No MeSH data available.


Related in: MedlinePlus