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Serum Pharmacochemistry Analysis Using UPLC-Q-TOF/MS after Oral Administration to Rats of Shenfu Decoction.

He JL, Zhao JW, Ma ZC, Wang YG, Liang QD, Tan HL, Xiao CR, Tang XL, Gao Y - Evid Based Complement Alternat Med (2015)

Bottom Line: Diester diterpenoid alkaloids were not present in the serum samples.A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation.We suspect that the increased absorption of the monoester diterpenoid alkaloids from the mixture of Panax and Radix, compared to the Panax only extract, may be the reason for the combination of the two herbs in popular medicine formulas in China.

View Article: PubMed Central - PubMed

Affiliation: Graduate School, Anhui Medical University, Hefei, China ; Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.

ABSTRACT
The purpose of this study was to study the serum pharmacochemistry of SFD as well as the material basis through analyzing the constituents absorbed in blood. The SFD was orally administrated to Wistar rats at 20 g·kg(-1), and Ultra Performance Liquid Chromatography (UPLC) fingerprints of SFD were created. Serum samples were collected for analysis, and further data processing used MarkerLynx XS software. 19 ginsenosides and 16 alkaloids were detected in SFD. The absorption of alkaloids (mainly monoester diterpenoid alkaloids) increased when Aconitum carmichaeli Debx. was combined with Panax ginseng, while the ginsenosides remained stable. Diester diterpenoid alkaloids were not present in the serum samples. A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation. This may also be useful for toxicity reduction. We suspect that the increased absorption of the monoester diterpenoid alkaloids from the mixture of Panax and Radix, compared to the Panax only extract, may be the reason for the combination of the two herbs in popular medicine formulas in China.

No MeSH data available.


Related in: MedlinePlus

Relative content of alkaloids detected in rats serum of C-Group and M-Group. C-Group: codecoction group; M-Group: mixed decoction group. A: cammaconine; B: carmichaeline; C: fuziline; D: neoline; E: talatizamine; F: acetyltalatizamine; G: mesaconine; H: 10-OH-benzoylmesaconine; M: benzoylmesaconine; N: dehydrated benzoylmesaconine; X: dehydrated benzoylhypaconine.
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fig7: Relative content of alkaloids detected in rats serum of C-Group and M-Group. C-Group: codecoction group; M-Group: mixed decoction group. A: cammaconine; B: carmichaeline; C: fuziline; D: neoline; E: talatizamine; F: acetyltalatizamine; G: mesaconine; H: 10-OH-benzoylmesaconine; M: benzoylmesaconine; N: dehydrated benzoylmesaconine; X: dehydrated benzoylhypaconine.

Mentions: The M-Group data showed that all alkaloids were absorbed more than the C-Group. The relative intensity of the ion indicated that the toxic chemicals (mesaconine, 10-OH-benzoylmesaconine, dehydrated benzoylmesaconine, and dehydrated benzoylhypaconine) were less absorbed than most general alkaloids in Aconitum plants (cammaconine, carmichaeline, fuziline, talatizamine, acetyltalatizamine, and neoline). The content of toxic alkaloids was higher in mixed decoction than codecoction which was probably the attenuation basis in vitro, and the results in vivo showed a similar consequence which indicated that the Renshen attenuated the toxicity of Fuzi. And ester exchange or degradation reactions occurring during the processing of codecoction with Renshen were considered to be the key factor of attenuation [5] (see Figure 7).


Serum Pharmacochemistry Analysis Using UPLC-Q-TOF/MS after Oral Administration to Rats of Shenfu Decoction.

He JL, Zhao JW, Ma ZC, Wang YG, Liang QD, Tan HL, Xiao CR, Tang XL, Gao Y - Evid Based Complement Alternat Med (2015)

Relative content of alkaloids detected in rats serum of C-Group and M-Group. C-Group: codecoction group; M-Group: mixed decoction group. A: cammaconine; B: carmichaeline; C: fuziline; D: neoline; E: talatizamine; F: acetyltalatizamine; G: mesaconine; H: 10-OH-benzoylmesaconine; M: benzoylmesaconine; N: dehydrated benzoylmesaconine; X: dehydrated benzoylhypaconine.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4530229&req=5

fig7: Relative content of alkaloids detected in rats serum of C-Group and M-Group. C-Group: codecoction group; M-Group: mixed decoction group. A: cammaconine; B: carmichaeline; C: fuziline; D: neoline; E: talatizamine; F: acetyltalatizamine; G: mesaconine; H: 10-OH-benzoylmesaconine; M: benzoylmesaconine; N: dehydrated benzoylmesaconine; X: dehydrated benzoylhypaconine.
Mentions: The M-Group data showed that all alkaloids were absorbed more than the C-Group. The relative intensity of the ion indicated that the toxic chemicals (mesaconine, 10-OH-benzoylmesaconine, dehydrated benzoylmesaconine, and dehydrated benzoylhypaconine) were less absorbed than most general alkaloids in Aconitum plants (cammaconine, carmichaeline, fuziline, talatizamine, acetyltalatizamine, and neoline). The content of toxic alkaloids was higher in mixed decoction than codecoction which was probably the attenuation basis in vitro, and the results in vivo showed a similar consequence which indicated that the Renshen attenuated the toxicity of Fuzi. And ester exchange or degradation reactions occurring during the processing of codecoction with Renshen were considered to be the key factor of attenuation [5] (see Figure 7).

Bottom Line: Diester diterpenoid alkaloids were not present in the serum samples.A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation.We suspect that the increased absorption of the monoester diterpenoid alkaloids from the mixture of Panax and Radix, compared to the Panax only extract, may be the reason for the combination of the two herbs in popular medicine formulas in China.

View Article: PubMed Central - PubMed

Affiliation: Graduate School, Anhui Medical University, Hefei, China ; Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.

ABSTRACT
The purpose of this study was to study the serum pharmacochemistry of SFD as well as the material basis through analyzing the constituents absorbed in blood. The SFD was orally administrated to Wistar rats at 20 g·kg(-1), and Ultra Performance Liquid Chromatography (UPLC) fingerprints of SFD were created. Serum samples were collected for analysis, and further data processing used MarkerLynx XS software. 19 ginsenosides and 16 alkaloids were detected in SFD. The absorption of alkaloids (mainly monoester diterpenoid alkaloids) increased when Aconitum carmichaeli Debx. was combined with Panax ginseng, while the ginsenosides remained stable. Diester diterpenoid alkaloids were not present in the serum samples. A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation. This may also be useful for toxicity reduction. We suspect that the increased absorption of the monoester diterpenoid alkaloids from the mixture of Panax and Radix, compared to the Panax only extract, may be the reason for the combination of the two herbs in popular medicine formulas in China.

No MeSH data available.


Related in: MedlinePlus