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Serum Pharmacochemistry Analysis Using UPLC-Q-TOF/MS after Oral Administration to Rats of Shenfu Decoction.

He JL, Zhao JW, Ma ZC, Wang YG, Liang QD, Tan HL, Xiao CR, Tang XL, Gao Y - Evid Based Complement Alternat Med (2015)

Bottom Line: Diester diterpenoid alkaloids were not present in the serum samples.A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation.We suspect that the increased absorption of the monoester diterpenoid alkaloids from the mixture of Panax and Radix, compared to the Panax only extract, may be the reason for the combination of the two herbs in popular medicine formulas in China.

View Article: PubMed Central - PubMed

Affiliation: Graduate School, Anhui Medical University, Hefei, China ; Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.

ABSTRACT
The purpose of this study was to study the serum pharmacochemistry of SFD as well as the material basis through analyzing the constituents absorbed in blood. The SFD was orally administrated to Wistar rats at 20 g·kg(-1), and Ultra Performance Liquid Chromatography (UPLC) fingerprints of SFD were created. Serum samples were collected for analysis, and further data processing used MarkerLynx XS software. 19 ginsenosides and 16 alkaloids were detected in SFD. The absorption of alkaloids (mainly monoester diterpenoid alkaloids) increased when Aconitum carmichaeli Debx. was combined with Panax ginseng, while the ginsenosides remained stable. Diester diterpenoid alkaloids were not present in the serum samples. A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation. This may also be useful for toxicity reduction. We suspect that the increased absorption of the monoester diterpenoid alkaloids from the mixture of Panax and Radix, compared to the Panax only extract, may be the reason for the combination of the two herbs in popular medicine formulas in China.

No MeSH data available.


Related in: MedlinePlus

Relative content of alkaloids detected in rats serum of F-Group and M-Group. F-Group: Fuzi decoction group; M-Group: mixed decoction group. A: cammaconine; B: carmichaeline; C: fuziline; D: neoline; E: talatizamine; F: acetyltalatizamine; K: benzoylaconine; G: mesaconine; H: 10-OH-benzoylmesaconine; M: benzoylmesaconine; N: dehydrated benzoylmesaconine; X: dehydrated benzoylhypaconine.
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fig6: Relative content of alkaloids detected in rats serum of F-Group and M-Group. F-Group: Fuzi decoction group; M-Group: mixed decoction group. A: cammaconine; B: carmichaeline; C: fuziline; D: neoline; E: talatizamine; F: acetyltalatizamine; K: benzoylaconine; G: mesaconine; H: 10-OH-benzoylmesaconine; M: benzoylmesaconine; N: dehydrated benzoylmesaconine; X: dehydrated benzoylhypaconine.

Mentions: After detecting these 14 alkaloids, we further processed the data. Comparing C-Group with F-Group, the absorption of MDAs (benzoylaconine, mesaconine, 10-OH-benzoylmesaconine, and dehydrated benzoylmesaconine) decreased and the general alkaloids in aconitum plants (cammaconine, neoline, and talatizamine) increased. Most alkaloids (cammaconine, carmichaeline, talatizamine, acetyltalatizamine, and dehydrated benzoylhypaconine) absorption in M-Group was increased rather than F-Group. The increased or decreased absorption of alkaloids may contribute to the attenuation and synergistic effects. The decreased absorption of toxic alkaloids leads to attenuation and increased nontoxic alkaloids contribute to synergistic effects mainly because of compatibility of Renshen. The phenomenon was found in ancient year by the Chinese and we make it clear by analyzing the chemical compounds (see Figures 5 and 6).


Serum Pharmacochemistry Analysis Using UPLC-Q-TOF/MS after Oral Administration to Rats of Shenfu Decoction.

He JL, Zhao JW, Ma ZC, Wang YG, Liang QD, Tan HL, Xiao CR, Tang XL, Gao Y - Evid Based Complement Alternat Med (2015)

Relative content of alkaloids detected in rats serum of F-Group and M-Group. F-Group: Fuzi decoction group; M-Group: mixed decoction group. A: cammaconine; B: carmichaeline; C: fuziline; D: neoline; E: talatizamine; F: acetyltalatizamine; K: benzoylaconine; G: mesaconine; H: 10-OH-benzoylmesaconine; M: benzoylmesaconine; N: dehydrated benzoylmesaconine; X: dehydrated benzoylhypaconine.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4530229&req=5

fig6: Relative content of alkaloids detected in rats serum of F-Group and M-Group. F-Group: Fuzi decoction group; M-Group: mixed decoction group. A: cammaconine; B: carmichaeline; C: fuziline; D: neoline; E: talatizamine; F: acetyltalatizamine; K: benzoylaconine; G: mesaconine; H: 10-OH-benzoylmesaconine; M: benzoylmesaconine; N: dehydrated benzoylmesaconine; X: dehydrated benzoylhypaconine.
Mentions: After detecting these 14 alkaloids, we further processed the data. Comparing C-Group with F-Group, the absorption of MDAs (benzoylaconine, mesaconine, 10-OH-benzoylmesaconine, and dehydrated benzoylmesaconine) decreased and the general alkaloids in aconitum plants (cammaconine, neoline, and talatizamine) increased. Most alkaloids (cammaconine, carmichaeline, talatizamine, acetyltalatizamine, and dehydrated benzoylhypaconine) absorption in M-Group was increased rather than F-Group. The increased or decreased absorption of alkaloids may contribute to the attenuation and synergistic effects. The decreased absorption of toxic alkaloids leads to attenuation and increased nontoxic alkaloids contribute to synergistic effects mainly because of compatibility of Renshen. The phenomenon was found in ancient year by the Chinese and we make it clear by analyzing the chemical compounds (see Figures 5 and 6).

Bottom Line: Diester diterpenoid alkaloids were not present in the serum samples.A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation.We suspect that the increased absorption of the monoester diterpenoid alkaloids from the mixture of Panax and Radix, compared to the Panax only extract, may be the reason for the combination of the two herbs in popular medicine formulas in China.

View Article: PubMed Central - PubMed

Affiliation: Graduate School, Anhui Medical University, Hefei, China ; Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.

ABSTRACT
The purpose of this study was to study the serum pharmacochemistry of SFD as well as the material basis through analyzing the constituents absorbed in blood. The SFD was orally administrated to Wistar rats at 20 g·kg(-1), and Ultra Performance Liquid Chromatography (UPLC) fingerprints of SFD were created. Serum samples were collected for analysis, and further data processing used MarkerLynx XS software. 19 ginsenosides and 16 alkaloids were detected in SFD. The absorption of alkaloids (mainly monoester diterpenoid alkaloids) increased when Aconitum carmichaeli Debx. was combined with Panax ginseng, while the ginsenosides remained stable. Diester diterpenoid alkaloids were not present in the serum samples. A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation. This may also be useful for toxicity reduction. We suspect that the increased absorption of the monoester diterpenoid alkaloids from the mixture of Panax and Radix, compared to the Panax only extract, may be the reason for the combination of the two herbs in popular medicine formulas in China.

No MeSH data available.


Related in: MedlinePlus