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Serum Pharmacochemistry Analysis Using UPLC-Q-TOF/MS after Oral Administration to Rats of Shenfu Decoction.

He JL, Zhao JW, Ma ZC, Wang YG, Liang QD, Tan HL, Xiao CR, Tang XL, Gao Y - Evid Based Complement Alternat Med (2015)

Bottom Line: The purpose of this study was to study the serum pharmacochemistry of SFD as well as the material basis through analyzing the constituents absorbed in blood.Diester diterpenoid alkaloids were not present in the serum samples.A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation.

View Article: PubMed Central - PubMed

Affiliation: Graduate School, Anhui Medical University, Hefei, China ; Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.

ABSTRACT
The purpose of this study was to study the serum pharmacochemistry of SFD as well as the material basis through analyzing the constituents absorbed in blood. The SFD was orally administrated to Wistar rats at 20 g·kg(-1), and Ultra Performance Liquid Chromatography (UPLC) fingerprints of SFD were created. Serum samples were collected for analysis, and further data processing used MarkerLynx XS software. 19 ginsenosides and 16 alkaloids were detected in SFD. The absorption of alkaloids (mainly monoester diterpenoid alkaloids) increased when Aconitum carmichaeli Debx. was combined with Panax ginseng, while the ginsenosides remained stable. Diester diterpenoid alkaloids were not present in the serum samples. A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation. This may also be useful for toxicity reduction. We suspect that the increased absorption of the monoester diterpenoid alkaloids from the mixture of Panax and Radix, compared to the Panax only extract, may be the reason for the combination of the two herbs in popular medicine formulas in China.

No MeSH data available.


Related in: MedlinePlus

Total ion chromatogram of codecoction. (a) Positive ion mode detected alkaloids, (b) negative ion mode detected ginsenosides.
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fig2: Total ion chromatogram of codecoction. (a) Positive ion mode detected alkaloids, (b) negative ion mode detected ginsenosides.

Mentions: The ginsenosides and alkaloids in SFD were identified using UPLC combined with a TOF-MS detector. The total ion chromatogram of SFD in positive and negative ion modes is shown in Figure 2 and was processed with MassLynx V4.1 (Waters, USA). Comparing the retention time and mass data with reference compounds identified the target compounds. Both the MDAs and DDAs of alkaloids and 20(s)-protopanaxadiol type, 20(s)-protopanaxatriol type, and oleanolic acid saponin-type ginsenosides were detected in SFD. The chemical compositions are shown in Tables 1 and 2.


Serum Pharmacochemistry Analysis Using UPLC-Q-TOF/MS after Oral Administration to Rats of Shenfu Decoction.

He JL, Zhao JW, Ma ZC, Wang YG, Liang QD, Tan HL, Xiao CR, Tang XL, Gao Y - Evid Based Complement Alternat Med (2015)

Total ion chromatogram of codecoction. (a) Positive ion mode detected alkaloids, (b) negative ion mode detected ginsenosides.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4530229&req=5

fig2: Total ion chromatogram of codecoction. (a) Positive ion mode detected alkaloids, (b) negative ion mode detected ginsenosides.
Mentions: The ginsenosides and alkaloids in SFD were identified using UPLC combined with a TOF-MS detector. The total ion chromatogram of SFD in positive and negative ion modes is shown in Figure 2 and was processed with MassLynx V4.1 (Waters, USA). Comparing the retention time and mass data with reference compounds identified the target compounds. Both the MDAs and DDAs of alkaloids and 20(s)-protopanaxadiol type, 20(s)-protopanaxatriol type, and oleanolic acid saponin-type ginsenosides were detected in SFD. The chemical compositions are shown in Tables 1 and 2.

Bottom Line: The purpose of this study was to study the serum pharmacochemistry of SFD as well as the material basis through analyzing the constituents absorbed in blood.Diester diterpenoid alkaloids were not present in the serum samples.A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation.

View Article: PubMed Central - PubMed

Affiliation: Graduate School, Anhui Medical University, Hefei, China ; Beijing Institute of Radiation Medicine, Tai-Ping Road 27, Beijing 100850, China.

ABSTRACT
The purpose of this study was to study the serum pharmacochemistry of SFD as well as the material basis through analyzing the constituents absorbed in blood. The SFD was orally administrated to Wistar rats at 20 g·kg(-1), and Ultra Performance Liquid Chromatography (UPLC) fingerprints of SFD were created. Serum samples were collected for analysis, and further data processing used MarkerLynx XS software. 19 ginsenosides and 16 alkaloids were detected in SFD. The absorption of alkaloids (mainly monoester diterpenoid alkaloids) increased when Aconitum carmichaeli Debx. was combined with Panax ginseng, while the ginsenosides remained stable. Diester diterpenoid alkaloids were not present in the serum samples. A suitable serum pharmacochemistry method was successfully established to study pharmacological effects and potential improvements in formulation. This may also be useful for toxicity reduction. We suspect that the increased absorption of the monoester diterpenoid alkaloids from the mixture of Panax and Radix, compared to the Panax only extract, may be the reason for the combination of the two herbs in popular medicine formulas in China.

No MeSH data available.


Related in: MedlinePlus