Limits...
Population Structure of and Conservation Strategies for Wild Pyrus ussuriensis Maxim. in China.

Wuyun T, Amo H, Xu J, Ma T, Uematsu C, Katayama H - PLoS ONE (2015)

Bottom Line: These values are relatively high when compared to those of other tree species.We determined 4 conservation units based on the clustering by nSSRs and cpSSRs, and geographic factor.This information is helpful in deciding the conservation strategies for wild Ussurian pear in China.

View Article: PubMed Central - PubMed

Affiliation: Paulownia Research and Development Center of China, Non-timber Forestry Research and Development of CAF, Weiwu Road, Zhengzhou City 450003, China.

ABSTRACT
Pyrus ussriensis Maxim. is native to the northern part of China, but whose habitats are currently being destroyed by environmental changes and human deforestation. An investigation of population structure and genetic diversity of wild Ussurian pear is a priority in order to acquire fundamental knowledge for conservation. A total of 153 individuals of wild Ussurian pear from the main habitats, Heilongjiang, Jilin, and Inner Mongolia in China, possessed low genetic diversity as a result of habitat fragmentation. The genetic diversity of the populations in Inner Mongolia and north east of Heilongjiang was especially low and there was the possibility of inbreeding. Wild Ussurian pears were divided into 5 groups based on the Bayesian clustering method using 20 nuclear SSRs (nSSRs) and 5 groups by haplotype distributions using 16 chloroplast SSRs (cpSSRs), and the populations in Inner Mongolia and north east of Heilongjiang represented unique genotypes. AMOVA indicated there was a 20.05% variation in nSSRs and a 44.40% variation in cpSSRs among populations. These values are relatively high when compared to those of other tree species. Haplotype E, positioned in the center of the cpSSR analysis network and showed the largest number of connections with other haplotypes, represented the most important haplotype. Inner Mongolia and the north east of Heilongjiang are two areas that need urgent conservation because of their genetic vulnerability and peculiarity. We determined 4 conservation units based on the clustering by nSSRs and cpSSRs, and geographic factor. This information is helpful in deciding the conservation strategies for wild Ussurian pear in China.

No MeSH data available.


Related in: MedlinePlus

Percentages of membership of genotypes to clusters (q value) inferred at K = 10 applying all samples including wild and cultivated pears to STRUCTURE analysis.Each genotype is represented by vertical line divided into colored segments. The length of the vertical lines indicates the proportions of genome attributed to the inferred clusters. * means cultivated pears.
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pone.0133686.g004: Percentages of membership of genotypes to clusters (q value) inferred at K = 10 applying all samples including wild and cultivated pears to STRUCTURE analysis.Each genotype is represented by vertical line divided into colored segments. The length of the vertical lines indicates the proportions of genome attributed to the inferred clusters. * means cultivated pears.

Mentions: Terakami et al [36] detected a total of 67 SSR regions (≧10 repeated motifs) by sequencing the entire chloroplast DNA of Pyrus pyrifolia. In this study, the software program, Primer3 (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi), was utilized to design primer pairs flanking SSRs. The major parameters for primer design were as follows: primer length, about 20 bp; PCR product size, between 100 to 250 bp; optimum annealing temperature, 60°C; GC content, about 50%. Synthesized primer pairs (Life Technologies Japan Ltd) were tested to detect polymorphisms within 95 individuals of wild Ussurian pear endemic in Japan, and 16 cpSSR primers were selected for the present study (Table 2 and S1 Table).


Population Structure of and Conservation Strategies for Wild Pyrus ussuriensis Maxim. in China.

Wuyun T, Amo H, Xu J, Ma T, Uematsu C, Katayama H - PLoS ONE (2015)

Percentages of membership of genotypes to clusters (q value) inferred at K = 10 applying all samples including wild and cultivated pears to STRUCTURE analysis.Each genotype is represented by vertical line divided into colored segments. The length of the vertical lines indicates the proportions of genome attributed to the inferred clusters. * means cultivated pears.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4529180&req=5

pone.0133686.g004: Percentages of membership of genotypes to clusters (q value) inferred at K = 10 applying all samples including wild and cultivated pears to STRUCTURE analysis.Each genotype is represented by vertical line divided into colored segments. The length of the vertical lines indicates the proportions of genome attributed to the inferred clusters. * means cultivated pears.
Mentions: Terakami et al [36] detected a total of 67 SSR regions (≧10 repeated motifs) by sequencing the entire chloroplast DNA of Pyrus pyrifolia. In this study, the software program, Primer3 (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi), was utilized to design primer pairs flanking SSRs. The major parameters for primer design were as follows: primer length, about 20 bp; PCR product size, between 100 to 250 bp; optimum annealing temperature, 60°C; GC content, about 50%. Synthesized primer pairs (Life Technologies Japan Ltd) were tested to detect polymorphisms within 95 individuals of wild Ussurian pear endemic in Japan, and 16 cpSSR primers were selected for the present study (Table 2 and S1 Table).

Bottom Line: These values are relatively high when compared to those of other tree species.We determined 4 conservation units based on the clustering by nSSRs and cpSSRs, and geographic factor.This information is helpful in deciding the conservation strategies for wild Ussurian pear in China.

View Article: PubMed Central - PubMed

Affiliation: Paulownia Research and Development Center of China, Non-timber Forestry Research and Development of CAF, Weiwu Road, Zhengzhou City 450003, China.

ABSTRACT
Pyrus ussriensis Maxim. is native to the northern part of China, but whose habitats are currently being destroyed by environmental changes and human deforestation. An investigation of population structure and genetic diversity of wild Ussurian pear is a priority in order to acquire fundamental knowledge for conservation. A total of 153 individuals of wild Ussurian pear from the main habitats, Heilongjiang, Jilin, and Inner Mongolia in China, possessed low genetic diversity as a result of habitat fragmentation. The genetic diversity of the populations in Inner Mongolia and north east of Heilongjiang was especially low and there was the possibility of inbreeding. Wild Ussurian pears were divided into 5 groups based on the Bayesian clustering method using 20 nuclear SSRs (nSSRs) and 5 groups by haplotype distributions using 16 chloroplast SSRs (cpSSRs), and the populations in Inner Mongolia and north east of Heilongjiang represented unique genotypes. AMOVA indicated there was a 20.05% variation in nSSRs and a 44.40% variation in cpSSRs among populations. These values are relatively high when compared to those of other tree species. Haplotype E, positioned in the center of the cpSSR analysis network and showed the largest number of connections with other haplotypes, represented the most important haplotype. Inner Mongolia and the north east of Heilongjiang are two areas that need urgent conservation because of their genetic vulnerability and peculiarity. We determined 4 conservation units based on the clustering by nSSRs and cpSSRs, and geographic factor. This information is helpful in deciding the conservation strategies for wild Ussurian pear in China.

No MeSH data available.


Related in: MedlinePlus