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Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent.

Machado BA, Barreto Gde A, Costa AS, Costa SS, Silva RP, da Silva DF, Brandão HN, da Rocha JL, Nunes SB, Umsza-Guez MA, Padilha FF - PLoS ONE (2015)

Bottom Line: The best results were identified at 50°C, 350 bar, 1% ethanol (co-solvent) and S/F of 110.These conditions, a content of 8.93±0.01 and 0.40±0.05 g/100 g of Artepillin C and p-coumaric acid, respectively, were identified indicating the efficiency of the extraction process.Despite of low yield of the process, the extracts obtained had high contents of relevant compounds, proving the viability of the process to obtain green propolis extracts with important biological applications due to the extracts composition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Research and Technology, Tiradentes University, Aracaju, Sergipe, Brazil; Faculty of Technology, SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil.

ABSTRACT
The aim of this study was to determine the best processing conditions to extract Brazilian green propolis using a supercritical extraction technology. For this purpose, the influence of different parameters was evaluated such as S/F (solvent mass in relation to solute mass), percentage of co-solvent (1 and 2% ethanol), temperature (40 and 50°C) and pressure (250, 350 and 400 bar) using supercritical carbon dioxide. The Global Yield Isotherms (GYIs) were obtained through the evaluation of the yield, and the chemical composition of the extracts was also obtained in relation to the total phenolic compounds, flavonoids, antioxidant activity and 3,5-diprenyl-4-hydroxicinnamic acid (Artepillin C) and acid 4-hydroxycinnamic (p-coumaric acid). The best results were identified at 50°C, 350 bar, 1% ethanol (co-solvent) and S/F of 110. These conditions, a content of 8.93±0.01 and 0.40±0.05 g/100 g of Artepillin C and p-coumaric acid, respectively, were identified indicating the efficiency of the extraction process. Despite of low yield of the process, the extracts obtained had high contents of relevant compounds, proving the viability of the process to obtain green propolis extracts with important biological applications due to the extracts composition.

No MeSH data available.


Isotherms for (A) concentration of p-coumaric acid; and (B) Artepillin C; on green propolis extracts obtained using CO2 as supercritical fluid, ethanol as co-solvent (1% m/m) at temperatures of 40 and 50°C and pressures of 250, 350 and 400 bar (CO2 flux of 6.0 g/min; time of extraction 2 h 30 min).
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pone.0134489.g008: Isotherms for (A) concentration of p-coumaric acid; and (B) Artepillin C; on green propolis extracts obtained using CO2 as supercritical fluid, ethanol as co-solvent (1% m/m) at temperatures of 40 and 50°C and pressures of 250, 350 and 400 bar (CO2 flux of 6.0 g/min; time of extraction 2 h 30 min).

Mentions: The influence of pressure and temperature for the extraction of p-coumaric acid and Artepilin C (μg/ml) are presented in Fig 8. On Table 3 are expressed the results obtained for the concentration of these two markers in μg/ml and g/100g for each extract, and also the results for the global yield (S6 Fig). These results identified these two markers of green propolis according to the isotherms presented on Figs 6 and 7.


Determination of Parameters for the Supercritical Extraction of Antioxidant Compounds from Green Propolis Using Carbon Dioxide and Ethanol as Co-Solvent.

Machado BA, Barreto Gde A, Costa AS, Costa SS, Silva RP, da Silva DF, Brandão HN, da Rocha JL, Nunes SB, Umsza-Guez MA, Padilha FF - PLoS ONE (2015)

Isotherms for (A) concentration of p-coumaric acid; and (B) Artepillin C; on green propolis extracts obtained using CO2 as supercritical fluid, ethanol as co-solvent (1% m/m) at temperatures of 40 and 50°C and pressures of 250, 350 and 400 bar (CO2 flux of 6.0 g/min; time of extraction 2 h 30 min).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4529176&req=5

pone.0134489.g008: Isotherms for (A) concentration of p-coumaric acid; and (B) Artepillin C; on green propolis extracts obtained using CO2 as supercritical fluid, ethanol as co-solvent (1% m/m) at temperatures of 40 and 50°C and pressures of 250, 350 and 400 bar (CO2 flux of 6.0 g/min; time of extraction 2 h 30 min).
Mentions: The influence of pressure and temperature for the extraction of p-coumaric acid and Artepilin C (μg/ml) are presented in Fig 8. On Table 3 are expressed the results obtained for the concentration of these two markers in μg/ml and g/100g for each extract, and also the results for the global yield (S6 Fig). These results identified these two markers of green propolis according to the isotherms presented on Figs 6 and 7.

Bottom Line: The best results were identified at 50°C, 350 bar, 1% ethanol (co-solvent) and S/F of 110.These conditions, a content of 8.93±0.01 and 0.40±0.05 g/100 g of Artepillin C and p-coumaric acid, respectively, were identified indicating the efficiency of the extraction process.Despite of low yield of the process, the extracts obtained had high contents of relevant compounds, proving the viability of the process to obtain green propolis extracts with important biological applications due to the extracts composition.

View Article: PubMed Central - PubMed

Affiliation: Institute of Research and Technology, Tiradentes University, Aracaju, Sergipe, Brazil; Faculty of Technology, SENAI/CIMATEC, National Service of Industrial Learning-SENAI, Salvador, Bahia, Brazil.

ABSTRACT
The aim of this study was to determine the best processing conditions to extract Brazilian green propolis using a supercritical extraction technology. For this purpose, the influence of different parameters was evaluated such as S/F (solvent mass in relation to solute mass), percentage of co-solvent (1 and 2% ethanol), temperature (40 and 50°C) and pressure (250, 350 and 400 bar) using supercritical carbon dioxide. The Global Yield Isotherms (GYIs) were obtained through the evaluation of the yield, and the chemical composition of the extracts was also obtained in relation to the total phenolic compounds, flavonoids, antioxidant activity and 3,5-diprenyl-4-hydroxicinnamic acid (Artepillin C) and acid 4-hydroxycinnamic (p-coumaric acid). The best results were identified at 50°C, 350 bar, 1% ethanol (co-solvent) and S/F of 110. These conditions, a content of 8.93±0.01 and 0.40±0.05 g/100 g of Artepillin C and p-coumaric acid, respectively, were identified indicating the efficiency of the extraction process. Despite of low yield of the process, the extracts obtained had high contents of relevant compounds, proving the viability of the process to obtain green propolis extracts with important biological applications due to the extracts composition.

No MeSH data available.